A5050698342- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Neuroscience and Neuropharmacology Research
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- Genomic variations and chromosomal abnormalities
- Ion channel regulation and function
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Health, Environment, Cognitive Aging
- Neuroscience and Neural Engineering
- RNA Research and Splicing
- Metabolism and Genetic Disorders
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Marine Toxins and Detection Methods
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Down syndrome and intellectual disability research
- Apelin-related biomedical research
- Renin-Angiotensin System Studies
University of Otago
2023-2024
University of Auckland
2013-2024
Maurice Wilkins Centre
2017-2024
National Institute on Aging
1997
National Institutes of Health
1981-1997
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1984-1994
McGill University
1989
University of California, Los Angeles
1982
Pancreatic neuroendocrine tumors (pNETs) are uncommon cancers arising from pancreatic islet cells. Here we report the analysis of gene mutation, copy number, and RNA expression 57 sporadic well-differentiated pNETs. pNET genomes dominated by aneuploidy, leading to concordant changes in at level whole chromosomes chromosome segments. We observed two distinct patterns somatic aneuploidy that associated with tumor pathology patient prognosis. Approximately 26% patients this series had pNETs...
Parallel electrophysiological and neurochemical studies of development are reported for mouse spinal cord cell cultures. The time course electrical activity the stage-dependent effects tetrodotoxin on levels neuronal enzyme choline acetyltransferase were compared to establish presence spontaneous at a when adversely affects development. extracellular patch electrode makes it possible examine ongoing small cells present in young A rapid increase during first 2 weeks culture was found...
The effects of chronic phenobarbital treatment on neuronal development were assessed in cell cultures fetal rodent spinal cord. activity the principal enzyme involved acetylcholine synthesis, choline acetyltransferase (CAT), and counts large cord neurons used as indicators Phenobarbital (30 to 120 micrograms/ml) added for two- or six-week periods produced dose-dependent decreases both CAT counts. Barbituric acid at doses equimolar those which these had no effect.
Background The nucleic acid-binding protein YB-1, a member of the cold-shock domain family, has been implicated in progression breast cancer and is associated with poor patient survival. YB-1 sequence similarity to LIN28, another family member, which role regulation small noncoding RNAs (sncRNAs) including microRNAs (miRNAs). Therefore, investigate whether there an association between sncRNAs cancer, we investigated were bound by two cell lines (luminal A-like basal cell-like), abundance...
Chemotherapy with taxanes such as paclitaxel (PTX) is a key component of triple negative breast cancer (TNBC) treatment. PTX used in combination other drugs both the adjuvant setting and advanced cancer. Because proportion patients respond poorly to or relapse after its use, greater understanding mechanisms conferring resistance required. One protein shown be involved drug Y-box binding 1 (YB-1). High levels YB-1 have previously been associated TNBCs. In this study, we aimed determine by...
The alpha 1-adrenergic receptor has been shown to mediate the release of arachidonic acid in FRTL5 thyroid cells and MDCK kidney cells. In primary cultures spinal cord cells, norepinephrine stimulated (from neurons only) turnover inositol phospholipids glia) via receptors. These two responses were dissociated by treatment with phorbol ester pertussis toxin, which inhibited production phosphates no appreciable effect on acid. Extracellular calcium was required for acid, but not phosphates....
Dissociated cultures from spinal cord-dorsal root ganglion were used as a model system to study the interaction between electrical activity and cyclic nucleotides on neuronal survival. Neuronal death produced by blockade with tetrodotoxin (TTX) was attenuated addition of 8-Bromo AMP (8-Br cAMP). [125I]Tetanus toxin fixation, choline acetyltransferase cell counts quantitate effects 8-Br cAMP cultures. A dose-dependent increase in both tetanus fixation (neuronal surface marker) observed...
Accurate assessment of chemotherapy response provides the means to terminate ineffective treatment, trial alternative drug regimens or schedules and reduce dose minimize toxicity. Here, we have compared circulating tumor DNA (ctDNA) with carcinoembryonic antigen (CEA) for cycle by in 30 patients metastatic colorectal cancer. CtDNA (quantified using individualized digital droplet PCR (ddPCR) assays) CEA levels were determined immediately prior each over time periods ranging from 42-548 days...
Circulating tumour DNA (ctDNA) analysis promises to improve the clinical care of people with cancer, address health inequities and guide translational research. This observational cohort study used ctDNA follow 29 patients advanced-stage cutaneous melanoma through multiple cycles immunotherapy.A melanoma-specific next-generation sequencing (NGS) panel, droplet digital polymerase chain reaction (ddPCR) mass spectrometry were identify mutations in longitudinal blood plasma samples from...
1. Calcium currents and transmitter release were studied in cocultures of fetal mouse neurons from the ventral half spinal cord (VH neurons) dorsal root ganglion (DRG neurons). The effects BayK 8644 omega-conotoxin on calcium compared. 2. presence low voltage-activated (LVA) current both VH DRG is variable. Some cells exhibit only high (HVA) currents, whereas others show HVA LVA currents. 3. did not affect but strongly augmented steady transient components conductance. 4. omega-Conotoxin...
TP53, the most commonly-mutated gene in cancer, undergoes complex alternative splicing. Different TP53 transcripts play different biological roles, both normal function and progression of diseases such as cancer. The study TP53’s RNA splice forms their use clinical biomarkers has been hampered by limited specificity quantitative accuracy current methods. variants differ at 5’ 3’ ends, but because they have a common central region 618 bp, individual are impossible to specifically detect...
Trisomy 21 (Down syndrome) is associated with a high incidence of Alzheimer disease and deficits in cholinergic function humans. We used the trisomy 16 (Ts16) mouse model for Down syndrome to identify cellular basis dysfunction. Cholinergic neurons cerebral cortical astroglia, obtained separately from Ts16 fetuses their euploid littermates, were cultured various combinations. Choline acetyltransferase activity neuron number both depressed cultures which glia derived fetuses. normal was...
Background: Liquid biopsies offer a minimally invasive approach to patient disease diagnosis and monitoring. However, these are highly affected by preprocessing variables with many protocols designed for downstream analysis of single molecular biomarker. Here we investigate whether specialized blood tubes could be repurposed the an increasingly valuable biomarker, extracellular vesicles (EVs). Methods: Blood was collected from three donors into K3-EDTA, Roche, or Streck cell-free DNA (cfDNA)...
Tumor evolution underlies many challenges facing precision oncology, and improving our understanding has the potential to improve clinical care. This study represents a rare opportunity tumor heterogeneity in patient with an understudied cancer type. A pulmonary atypical carcinoid, neuroendocrine tumor, metastatic 90 sites, requested consented donate tissues for research. 42 samples collected at rapid autopsy from 14 anatomically distinct sites were analyzed through DNA whole-exome...
We report the results of a pilot project for clinical DNA sequencing in New Zealand. This aimed to estimate diagnostic yield next generation Zealand environment. Trio whole exome (WES) was performed on germline 40 individuals from 12 families with presumptive Mendelian disorders. In addition, both WES and deep targeted (DTS) tumours, metastases corresponding normal blood leukocytes two cancer patients. For rare disorder cohort, 6/12, including previously recognised pathogenic mutations novel...
Abstract Background & Methods: In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR Illumina DNA methylation array. Results: The RE cg05575921 appeared concordant BIS (r 2 =0.94, P<0.0001) when array, had significantly better smoking status classification performance current versus never...
In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR Illumina DNA methylation array. The RE cg05575921 appeared concordant BIS (r2 = 0.94, P < 0.0001) and, when array, had significantly better smoking status classification performance current versus never smoked (AUC 0.96 0.93, 0.04) ex-smoker...