A5101414515- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Cancer, Lipids, and Metabolism
- Genetic factors in colorectal cancer
- Metabolomics and Mass Spectrometry Studies
- DNA Repair Mechanisms
- Ginger and Zingiberaceae research
- Microfluidic and Capillary Electrophoresis Applications
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and disorders
- Glutathione Transferases and Polymorphisms
- Fungal Plant Pathogen Control
- Nutrition, Genetics, and Disease
- BRCA gene mutations in cancer
- Effects of Radiation Exposure
- Cholesterol and Lipid Metabolism
- Clusterin in disease pathology
- Lipoproteins and Cardiovascular Health
- Insect Pheromone Research and Control
- Advanced Chemical Sensor Technologies
- Coconut Research and Applications
- Endoplasmic Reticulum Stress and Disease
- Physiological and biochemical adaptations
- Food Chemistry and Fat Analysis
- Inflammatory Bowel Disease
Sanya University
2024
Hainan University
2021-2024
Gansu Provincial Hospital
2014-2024
Gansu Provincial Cancer Hospital Gansu Provincial Academic Instiute for Medical Research
2014-2023
Academy of Medical Sciences
2023
Wenzhou Medical University
2019
First Affiliated Hospital of Wenzhou Medical University
2019
Merck & Co., Inc., Rahway, NJ, USA (United States)
2012-2015
Northeastern University
2000
A new method is presented for quantifying proteomic and metabolomic profile data by liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization. This biotechnology provides differential expression measurements enables the discovery of biological markers (biomarkers). Work here uses human serum but applicable to any fluid or tissue. The approach relies on linearity signal versus molecular concentration reproducibility sample processing. There no use isotopic labeling...
Volatile accumulation during tomato ripening greatly affects the fruit flavor. In this study, four accessions from each of three subgroups (BIG, S. lycopersicum, CER, lycopersicumvar. Cerasiforme, and PIM, pimpinellifolium) were subjected to a sensory evaluation. The CER subgroup had highest fruit-flavor score. Using Headspace solid-phase microextraction/gas chromatography-mass spectrometer (HP-SPME/GC-MS), volatile database containing 94 volatiles was created. Pentanal accumulated in green...
Zingiberaceae plants are widely used in the food and pharmaceutical industries; however, research on chemical composition interspecific differences metabolome volatilome of is still limited. In this study, seven species were selected, including Curcuma longa L., Zingiber officinale Rosc., Alpinia officinarum Hance, tonkinensis Gagnep, Amomum tsaoko Crevost et Lemarie, hainanensis K. Schum. villosum Lour. Myristica fragrans Houtt. was also selected due to its flavor being similar that plant....
A modular subatmospheric electrospray interface with fiber optic UV detection close to the tip was developed for coupling of microcolumn separation techniques mass spectrometry. The based on a liquid junction removable microelectrospray tip. enclosed in chamber attached front sampling orifice spectrometer. inlet maintained at atmospheric pressure, and thus no pressure drop across column. flow rate electrosprayed from reservoir adjusted by chamber. In this approach, continuous stable could be...
C-reactive protein (CRP) is an established marker of inflammation with pattern-recognition receptor-like activities. Despite the close association serum level CRP risk and prognosis several types cancer, it remains elusive whether contributes directly to tumorigenesis or just represents a bystander marker. We have recently identified recurrent mutations at SNP position -286 (rs3091244) in promoter gene tumor types, instead suggesting that locally produced potential driver tumorigenesis....
Stable isotope tracer studies of apoprotein flux in rodent models present difficulties as they require working with small volumes plasma. We demonstrate the ability to measure by administering either <sup>2</sup>H- or <sup>18</sup>O-labeled water mice and then subjecting samples LC-MS/MS analyses; we were able simultaneously determine labeling several proteolytic peptides representing multiple apoproteins. Consistent relative differences reported literature regarding humans, found that...
LC/MS quantification of multiple plasma proteins that differ by several orders magnitude in concentration from a single sample is challenging. We present strategy allows the simultaneous determination and turnover kinetics higher lower abundant digestion mixture. Our attention was directed at cluster interact to affect absorption interorgan lipid trafficking. demonstrate apos involved TG metabolism such as apoC2, C3, E, A4 (micromolar concentration), apoB48 apoA5 (single-digit nanomolar...
The synthesis of various molecules can be estimated by measuring the incorporation a labeled precursor into product interest. Unfortunately, central problem in many studies has been an inability to estimate intracellular dilution and therein correctly calculate product; it is generally assumed that true labeling straightforward. We initiated study examine liver collagen identified apparent with assumptions regarding measurements labeling. Since well known production relatively slow, we...
Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS UHPLC-MS/MS in two (Hainan Tall, HT Green Dwarf, GD). Four metabolite classes of associated with good aroma including hydrocarbons, benzenoids, alcohols esters, these generally higher GD, especially 7 9 months growth. Pathway-based metabolomics revealed...
RATIONALE Human genetics studies in African Americans have shown a strong correlation between polymorphisms the ApoL1 gene and chronic kidney disease (CKD). To gain further insight into etiology of ApoL1‐associated diseases, determination circulating levels both wild type as well variants could be significant use. date, antibodies that discriminate all three ApoL1variant forms (wild type, G1 G2) are not available. We aimed to develop rapid method for detecting quantifying total plasma....
Ovarian cancer is one of the most lethal malignancies, while targeting apoptosis critical for improving ovarian patients' lives. Survivin regarded as robust anti-apoptosis proteins, and its overexpression in associated with resistance poor survival. Piperlongumine (PL) a biologically active alkaloid/amide from peppers exhibits broad spectrum antitumor effects. Here, we demonstrate that PL rapidly reduces survivin levels cells via reactive oxygen species (ROS)-mediated proteasome-dependent...
Genetic variations in key DNA repair genes may influence capacity, damage and breast carcinogenesis. The current study aimed to estimate the association of APEX1 OGG1 polymorphisms with risk cancer development. A total 518 patients histopathologically confirmed 921 region- age-matched cancer-free controls were genotyped for rs3136817 rs1130409 rs1052133 rs2072668 using a QuantStudio™ 12 K Flex Real-Time PCR System. heterozygous TC genotype along dominant model (TC + CC) was strongly...
Circulating miRNAs presenting in plasma a stable manner have been demonstrated their potential role as promising biomarkers many human diseases, such Alzheimer's disease, melanoma and ovarian carcinoma. However, few circulating could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p biomarker detecting cancer. We detected levels the of 39 sporadic patients 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed level were...
Xanthohumol (XN) is the major prenylated chalcone of female inflorescences (cone) hop plant (Humulus lupulus). It also a constituent beer, dietary source flavonoids. has shown strong antitumorigenic activity towards various types cancer cells. In present study, we show impact on human hepatocarcinoma cell line HepG2 and potential adverse effects rat primary hepatocytes. Cell growth/viability assay (MTT) demonstrated that cells are highly sensitive to XN at concentration range 25-100 μM. The...
Intracellular proteins are in a state of flux, continually being degraded into amino acids and resynthesized new proteins. The rate this biochemical recycling process varies across is emerging as an important consideration drug discovery development. Here, we developed triple-stage quadrupole mass spectrometry assay based on product ion measurements at unit resolution H(2)(18)O stable tracer incorporation to measure relative protein synthesis rates. As proof concept, selected the vivo...
: C-reactive protein (CRP) functions as a nonspecific marker in various inflammatory disorders, particularly evaluating the efficacy of pharmacological treatments patients with ulcerative colitis. The existing body evidence does not offer adequate support for direct implication CRP modulating advancement
Abstract Background The XRCC2 gene plays an important role in the homologous recombination repair of DNA double strand breaks. Functional single nucleotide polymorphisms (SNPs) may alter capacity and affect cancer susceptibility. This study aims to evaluate association between five tagSNPs breast susceptibility Han women northwest China. Methods A total 514 histopathologically confirmed patients 1028 region- age-matched cancer-free controls provided blood samples for analysis SNP genotypes...
Background: The association of genetically elevated levels circulating C-reactive protein (CRP) with cancer risk has been extensively investigated in European populations; however, there are conflicting conclusions. tri-allelic rs3091244 is a functionally validated genetic variant, and its allelic frequencies differ significantly between Asian populations. Here, we examined the Chinese population. Methods: was genotyped by Sanger sequencing 4971 cases 2485 controls. rs1205 rs2794521 gene...
A modular subatmospheric electrospray interface with fiber optic UV detection close to the tip was developed for coupling of microcolumn separation techniques mass spectrometry. The based on a liquid junction removable microelectrospray tip. enclosed in chamber attached front sampling orifice spectrometer. inlet maintained at atmospheric pressure, and thus no pressure drop across column. flow rate electrosprayed from reservoir adjusted by chamber. In this approach, continuous stable could be...
Investigations surrounding dyslipidemia often examine lipid (e.g. triglyceride) and protein apoB) kinetics in separate studies which is unfortunate since the pathways are related. We recently demonstrated ability to quantify flux vivo using H 2 18 O; hydrolysis of pre‐existing proteins O generates labeled amino acids, thus synthesis estimated from incorporation those acids into newly made proteins. now demonstrate a similar logic for studying synthesis, i.e. degradation lipids labels fatty...
We recently demonstrated the ability to simultaneously quantify lipid and protein flux in vivo using oral D 2 O dosing. The uniqueness of is rapid equilibration with total body water, use not intravenous dosing, ease measure true precursor enrichment (either plasma, urine, or saliva). To examine whether dietary modulation de novo fatty acid cholesterol synthesis would lead parallel changes apoB and/or A1 production, non‐human primates were either fed a standard carbohydrate‐based diet...