A5053962086- Colorectal Cancer Surgical Treatments
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Anorectal Disease Treatments and Outcomes
- Gastrointestinal disorders and treatments
- Intestinal and Peritoneal Adhesions
- Cytokine Signaling Pathways and Interactions
- Gastric Cancer Management and Outcomes
- Hernia repair and management
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Enhanced Recovery After Surgery
- Cerebrovascular and genetic disorders
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Gastrointestinal Bleeding Diagnosis and Treatment
- Pelvic floor disorders treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastrointestinal Tumor Research and Treatment
- Colorectal and Anal Carcinomas
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Medical Device Sterilization and Disinfection
Renmin Hospital of Wuhan University
2013-2023
Wuhan University
2013-2023
Xiang Yang No.1 People's Hospital
2013
AIM: To examine the potential anti-tumor activity of paeoniflorin in human gastric carcinoma cell line MGC-803. METHODS:Cell viability and cytotoxic effects MGC-803 cells were analyzed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide lactate dehydrogenase assay, respectively.Cell apoptosis was measured flow cytometry, DAPI staining assay caspase-3 assay.Quantitative reverse transcription-polymerase chain reaction (RT-PCR) used to measure expression microRNA-124 (miR-124)...
Colorectal cancer (CRC) ranks the third most common type of worldwide. However, detailed molecular mechanisms underlying these processes are poorly understood. Recent studies have shown that lncRNAs play important roles in carcinogenesis and progression CRC. The lncRNA growth arrest special 5 (GAS5), was previously identified to be down-regulated functions as a tumor suppressor gene many kinds cancers. In current two-stage, case-control study, we systematically evaluated potential role GAS5...
Colorectal cancer (CRC) has become the third most common worldwide and leads to a high mortality rate. Although colorectal been studied widely, underlying molecular mechanism remains unclear. Increasing evidence shows that abnormal expression of microRNAs (miRNAs) is involved in tumorigenesis. Previous studies have reported miRNA-103 (miR-103) dysregulated CRC; however, expression, function miR-103 CRC are not well known. The present study showed miR‑103 was overexpressed primary tumor...
// Yongbin Zheng 1 , Chao Yang Shilun Tong Yu Ding Wenhong Deng Dan Song Kuang Xiao Department of Gastrointestinal Surgery, Renmin Hospital Wuhan University, Wuhan, Hubei, PR China Correspondence to: Zheng, email: zhengyongbinhb@163.com Keywords: colorectal cancer, variation, genetic, TINCR, lncRNA Received: January 05, 2017 Accepted: February 13, Published: March 24, ABSTRACT Colorectal cancer (CRC) accounts for the leading causes cancer-related morbidity and mortality. However, a large...
Sexual dysfunctions seriously affect the quality of life patients. The aim this study was to identify risk factors for sexual dysfunction after rectal cancer surgery.A total 948 consecutive patients undergoing radical resection were included between January 2012 and August 2019. functions evaluated by 5-item version International Index Erectile Function (IIEF-5) in men Female (IFSF) women at 12 months postoperatively.Postoperative observed 228 with (24.05%), which 150 cases male (25.0%) 78...
Focal adhesion kinase (FAK) is suggested to be intimately involved in the progression of malignancies. Our previous research has demonstrated that activation cholecystokinin-2 receptor (CCK2R) by gastrin stimulates a rapid FAK pathway human colon cancer cells. The purpose this study determine role CCK2R and cancer. In study, matched tissue samples primary adjacent normal mucosa from same patient were collected 45 patients with undergoing surgical resection. expression was detected using...
Metastasis and invasion are the main causes of mortality in gastric cancer. To improve treatment cancer, development effective innovative antitumor agents toward proliferation is needed. Alpha-lipoic acid (ALA), a naturally occurring thiol antioxidant, showed antiproliferative cytotoxic effects on several cancers. So it feasible to explore whether ALA can be used inhibit human The expression MUC4 cancer tissues was assayed by immunohistochemistry. Then, we performed vitro cell analysis...
Accumulating studies have shown that the dysregulation of microRNAs is related to carcinogenesis and development gastric cancer (GC), role miR-635 in GC remains largely unknown. Kinesin Family Member C1 (KIFC1) mRNA expression tissues paracancerous cells were detected by quantitative real-time PCR. KIFC1 protein normal was immunohistochemistry western blot. Cell proliferation monitored Counting Kit-8 assay 5-bromo-2′-deoxyuridine assay. Transwell employed detect migration invasion cells. The...
Background . As a key step in enhancing cancer cell invasion and metastasis, epithelial-mesenchymal transition (EMT) plays an important role colorectal progression. EMT is triggered by variety of signaling pathways, among which the transforming growth factor β (TGF- ) pathway has been implicated as primary inducer. Accumulating evidence demonstrates that MnTE-2-PyP (chemical name: manganese(III) meso -tetrakis-(N-ethylpyridinium-2-yl), superoxide dismutase (SOD) mimetic, inhibits TGF-...
In patients with gastric cancer (GC), peritoneal metastasis is an indication of the end stage and often indicates a poor outcome. The diagnosis metastasis, especially occult (OPM), remains challenge for surgeons. This study was designed to explore relationship between OPM clinicopathological characteristics preoperative hematological parameters in GC develop nomogram predict probability before surgery.A total 672 from our center were included, including 583 OPM-negative 89 OPM-positive...
Angiogenesis depends on interaction between a variety of promoting and inhibiting factors, is known to involve vascular endothelial growth factor (VEGF) A the Notch signaling pathway. The present study investigated expression ligand delta-like (DLL) 4 (Drosophila), VEGFA in colon cancer colorectal adenoma tissue, association with tumour angiogenesis.Protein level DLL4, CD34 molecule (CD34) was detected immunohistochemically tissue sections from patients adenoma.Out 80 cases (35 cancer, 45...
Urinary and sexual dysfunctions are among the most common complications in rectal cancer surgery. This study aimed to investigate protective effect of laparoscopic functional total mesorectum excision (TME) on urinary functions male patients.A 248 patients with mid-low were recruited this between February 2017 July 2020. To overcome selection bias, we performed a 1:1 match using six variables, including age, BMI, ASA score, tumor distance, clinical T stage, size. The function was assessed by...
Station 253 node dissection with high ligation of the inferior mesenteric artery (IMA) is difficult to perform without damage surrounding autonomic nerve plexuses. This study aimed investigate significance plane for plexus (IMP) preservation in laparoscopic rectal cancer surgery.A total 56 consecutive patients underwent en bloc station IMA. nodes were divided into extra- and intra-nerve further H&E staining immunohistochemical analysis. Based on IMP plane-based evidence histopathological...
Rectal cancer is one of the malignant diseases with high morbidity and mortality in world. Currently, surgical resection main treatment method, preoperative chemoradiotherapy (CRT) widely used clinical application to increase resectability decrease local recurrence rate. However, CRT increases risk colon anastomotic leak, currently, there are no FDA approved treatments against this side effect. It essential develop new drugs reduce postoperative leak after CRT. 90 rats underwent standard...
To explore HtrA1 gene expression and its regulation in human gastric cancers. The mRNA levels were examined by QPCR analysis confirmed with Northern blot analysis. protein all six epithelial cell lines investigated Western Gene copy number was accessed then sequenced the coding region from each lines. promoter DNA methylation status detected using bisulfite sequencing Effect of decitabine TSA on HTRA1 cancer line determined RTPCR. HIC indicated that highly expressed normal epithelium, but...
Objective: Using previous total mesorectal excision with pelvic autonomic nerve preservation (PANP+TME) and simple (TME) without emphasis on retained nerves as control, we explore the advantages of plane-oriented laparoscopic (NPO+LTME) urinary sexual function. Methods: A retrospective cohort study was carried out. Case inclusion criteria: (1) male patients pathologically confirmed middle low rectal adenocarcinoma (4 to 11 cm from anus); (2) stage T1-2tumor; (3) normal life before operation....