A5034796819- Higher Education and Teaching Methods
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Kidney Stones and Urolithiasis Treatments
- Mesenchymal stem cell research
- Total Knee Arthroplasty Outcomes
- Cancer, Hypoxia, and Metabolism
- Orthopaedic implants and arthroplasty
- Cancer-related gene regulation
- Osteoarthritis Treatment and Mechanisms
- Educational Systems and Policies
- interferon and immune responses
- Genetic factors in colorectal cancer
- Circular RNAs in diseases
- Cancer, Lipids, and Metabolism
- Educational Research and Pedagogy
- Ideological and Political Education
- Cytokine Signaling Pathways and Interactions
- Educational Technology and Assessment
- Ferroptosis and cancer prognosis
- Pancreatitis Pathology and Treatment
- Abdominal Surgery and Complications
- TGF-β signaling in diseases
- Gout, Hyperuricemia, Uric Acid
- Ginger and Zingiberaceae research
Ganzhou People's Hospital
2019-2024
Nanchang University
2021-2024
Union Hospital
2017-2024
Huazhong University of Science and Technology
2008-2024
Affiliated Hospital of Jining Medical University
2019-2024
Union Hospital
2024
Wuyi University
2024
Guangzhou University of Chinese Medicine
2013-2023
Sichuan University of Science and Engineering
2022
Johns Hopkins Medicine
2019-2022
Circular RNA (circRNA) is related to many human diseases including osteoarthritis (OA). Our research purpose was show that functional circRNAs have a role in the pathogenesis of OA, while also identifying potential circRNA bind miRNA-27b-3p. Microarray analysis used evaluate expression CircRNA OA and normal cartilage. The mechanism Circ_0000423 up-regulation were detected verified vitro vivo. transfection, qRT-PCR, Western blot analysis, immunofluorescence, dual-luciferase assays investigate...
There is no report about the direct relationship between m6A modification and androgen receptor (AR)-related genes in prostate cancer (PC). We aimed to study mechanisms of methylation regulating pathogenesis PC from perspective AR-related genes.qRT-PCR was applied detect expression m6A-related cell with or without AR inhibitor. The effects YTHDF1 knockdown on viability, apoptosis, migration invasion were investigated using flow cytometry, wound healing transwell assays, respectively....
This study was performed to screen miRNAs and mRNAs that are differentially expressed during trypsinogen activation in acute pancreatitis verify their role the process of activation. The function enrichment analysis showed functions miR-352 its regulatory targets lysosome-associated membrane protein 2 (LAMP2) cathepsin L1 (CTSL1) were lysosome related. results verification experiment TLC-S-treated AR42J (pancreatic cell line) cells, expression increased, levels LAMP2 CTSL1 significantly...
// Yulin Liu 1, * , Fan Xiang Yongming Huang 1 Liang Shi 2 Chaojie Hu Yiming Yang Di Wang Nan He Kaixiong Tao Ke Wu Guobin Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Wuhan 430022, China Clinical Laboratory, These authors have contributed equally to this work Correspondence to: Wang, email: guobinwanguh@163.com Wu, wuke_2015@yeah.net Keywords: interleukin-22, colon cancer, aerobic glycolysis, proliferation,...
Abstract Background Colitis-associated colon cancer (CAC) patients have a younger age of onset, more multiple lesions and invasive tumors than sporadic patients. Early detection CAC using endoscopy is challenging, the incidence septal remains high. Therefore, identifying biomarkers that can predict tumorigenesis in urgent need. Results A total 275 DEGs were identified CAC. IGF1 , BMP4 SPP1 APOB CCND1 CD44 PTGS2 CFTR BMP2 KLF4 TLR2 as hub DEGs, which significantly enriched PI3K-Akt pathway,...
Previous studies have reported that TIFA plays different roles in various tumor types. However, the function of colorectal cancer (CRC) remains unclear. Here, we showed expression was markedly increased CRC versus normal tissue, and positively correlated with TNM stages. In agreement, found cell lines show levels control. The knockdown inhibited proliferation but had no effect on apoptosis vitro or vivo. Moreover, ectopic enhanced ability contrast, mutant (T9A, oligomerization site mutation;...
Background . As a key step in enhancing cancer cell invasion and metastasis, epithelial-mesenchymal transition (EMT) plays an important role colorectal progression. EMT is triggered by variety of signaling pathways, among which the transforming growth factor β (TGF- ) pathway has been implicated as primary inducer. Accumulating evidence demonstrates that MnTE-2-PyP (chemical name: manganese(III) meso -tetrakis-(N-ethylpyridinium-2-yl), superoxide dismutase (SOD) mimetic, inhibits TGF-...
Liver cirrhosis is recognized as being the consequence of immune-mediated hepatocyte damage and repair processes. However, regulation these immune responses underlying liver has not been elucidated. In this study, we used GEO datasets bioinformatics methods to established coding non-coding gene regulatory networks including transcription factor-/lncRNA-microRNA-mRNA, competing endogenous RNA interaction networks. Our results identified 2224 mRNAs, 70 lncRNAs 46 microRNAs were differentially...
Chemotherapeutic agent-induced nausea and vomiting are the severe adverse effects that induced by their stimulations on peripheral and/or central emetic nerve pathways. Even though ginger has been widely used as an herbal medicine to treat emesis, mechanisms underlying its neuronal actions still less clear. The present study aimed determine chemotherapeutic agent vincristine-induced effect gastroesophageal vagal afferent endings potential inhibitory role of constituent 6-shogaol such...
Purpose: CD24 is overexpressed in hepatocellular carcinoma (HCC) tumor tissues and the highly metastatic HCC cell lines. However, plasma level patients correlation of with clinicopathological factors prognosis still remain unclear. Materials Methods: Enzyme-linked immunosorbent assay was used to detect 86 patients, 35 healthy subjects, 26 liver cirrhosis 23 chronic hepatitis. The relationship between characteristics assessed using Mann–Whitney U test. Patient survival groups evaluated by...
To the Editor: Hyperuricemia, second most common metabolic disease after diabetes mellitus in China, is characterized by elevated serum uric concentration which due to overactive acid production or underexcretion.[1] Recent study supported notion that hyperuricemia implicated renal tubular injury, subsequent tubulointerstitial fibrosis, and finally gouty nephropathy.[2] However, lack of a suitable stable mouse model gout nephropathy delays limits development mechanism research new drugs....
Abstract Traumatic spinal cord injury (TSCI) is a prevalent central nervous system condition that imposes significant burden on both families and society, affecting more than 2 million people worldwide. Recently, there has been increasing interest in bone marrow mesenchymal stem cell (BMSC) transplantation as promising treatment for (SCI) due to their accessibility low immunogenicity. However, the mere of BMSCs limited capacity directly participate repair host nerve function. MiR-28-5p,...
Abstract Background Ginger has been used as an herbal medicine worldwide to relieve nausea/vomiting and gastrointestinal discomfort, but the cellular molecular mechanisms of its neuronal action remain unclear. The present study aimed determine effects ginger constituent 6‐shogaol on gastroesophageal vagal nodose C‐fibers. Methods Extracellular single‐unit recording two‐photon neuron imaging were performed, respectively, in ex vivo gastroesophageal‐vagal preparations from wild type...
Abstract Peritoneal adhesion occurs frequently after gastrointestinal/gynecological surgery. Tissue repair and regeneration are very important during this process. IL‐22 is an cytokine that secreted from immune cells but functions on mesenchymal cells, such as mesothelial cells. The objective of study was to investigate the roles its regulators formation. Postsurgical peritoneal drainage fluid patients rodent models examined by enzyme‐linked immunosorbent assay fluorescence‐activated cell...
This study aimed to demonstrate that dual-mobility cup total hip arthroplasty (DMC-THA) can significantly improve the quality of life (QOL) elderly femoral neck fracture patients with severe neuromuscular disease in unilateral lower extremities due stroke hemiplegia compared internal fixation (IF).Fifty-eight cases muscle strength < grade 3/5 were retrospectively examined From January 2015 December 2020. Then, divided into DMC and IF groups. The QOL was using EQ-5D SF-36 outcome measures....
Abstract Rationale: Less-Invasive Stabilization System (LISS) plate is an internal fixation commonly used for the periprosthetic distal femur fractures. Failure associated with LISS has been rarely reported, and reasons failure are multitudinous. Various advantages have but failures continue. Patient concerns: We present 3 cases illustrating of plating The shaft screws broke in 2 cases, placement was incorrect 1 case. Early weight bearing, obesity, osteoporosis, lateral collateral ligament...
Objectives In this study, we identified the protein kinases that play most distinct roles in occurrence of acute pancreatitis (AP). Methods Gene expression profile data were downloaded from Expression Omnibus database (GSE3644). The sample was caerulein-induced AP mice. intersection differentially expressed genes mice taken a kinase obtained for screening encoded expressed. Database annotation, visualization, and integrated discovery used functional enrichment analysis. Kinase inhibitors...
Fistula-in-ano (FIA, anal fistula) treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are new frontier in the of FIA. In this study, we established FIA model Japanese rabbit and evaluated effect four treatments on fistula healing: ADSC transplantation, acellular small intestinal submucosa (ASIS), noncutting seton, PBS as negative control. High-throughput RNA sequencing was also performed to investigate tissue (normal group) expression...
Abstract Background To test the reliability and safety of a newly invented technique for minimally invasive percutaneous nephrolithotomy, intelligent pressure-controlled nephrolithotomy (IPC-MPCNL). Methods Eighteen kidneys nine female pigs were randomly divided into three groups. Those in Groups A B underwent IPC-MPCNL through new system composed pressure-measuring MPCNL suctioning sheath an irrigation platform with pressure feedback control. The infusion flow rate was 500 ml/min Group 750...