Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) has been reported to play a role in the suppression of activated hepatic stellate cells (HSCs). Moreover, it demonstrated that hypermethylation PTEN promoter is responsible for loss expression during HSC activation. Methylation now established as fundamental regulator gene transcription. MicroRNAs (miRNAs), which can control by binding their target genes degradation and/or translational repression, were found be involved liver...

// Jie You 1, 2, * , Gui-Qi Zhu 3, 4, Linka Xie 5, Wen-Yue Liu 6 Liang Shi 7 Ou-Chen Wang 1 Zong-Hai Huang 2 Martin Braddock 8 Gui-Long Guo Ming-Hua Zheng 9 Department of Oncological Surgery, The First Affiliated Hospital Wenzhou Medical University, 325000, China General Zhujiang Hospital, Southern Guangzhou 510282, 3 Infection and Liver Diseases, Research Center, 4 School Clinical Sciences, 5 Cancer Center Union Tongji College, Huazhong University Science Technology, Wuhan 430022,...

5-Fluorouracil (5-FU) has been widely applied to treat various types of cancers, including hepatocellular carcinoma (HCC). However, primary or acquired 5-FU resistance prevents the clinical application this drug in cancer therapy. Herein, our study is first demonstrate that lower expression KRAL, a long non-coding RNA (lncRNA), mediates HCC via miR-141/Keap1 axis. Cell proliferation assays, western blot analysis, qRT-PCR, dual-luciferase reporter assay and immunoprecipitation were performed...

Nuclear factor-kappaB (NF-kappaB) plays a major role in host inflammatory responses and carcinogenesis as such is an important drug target for adjuvant therapy. In this study, we examined the effect of caffeic acid phenethyl ester (CAPE), NF-kappaB inhibitor, on Helicobacter pylori (H. pylori)-induced activation cell culture chronic gastritis Mongolian gerbils. AGS gastric cancer cells, CAPE significantly inhibited H. pylori-stimulated mRNA expression several factors dose-dependent manner,...

Abstract Background Spices have been used for thousands of years, and recent studies suggest that certain spices confer beneficial effects on gastric disorders. The purpose this study was to evaluate possible chemopreventive spice‐derived compounds H elicobacter pylori ( . )‐induced gastritis. Methods We examined the inhibitory curcumin, capsaicin, piperine in vitro by determining colony‐forming units real‐time RT ‐ PCR H. stimulated AGS cancer cells. For vivo analysis, 6‐week‐old SPF male M...

Abstract Background Helicobacter pylori ( H. ) infection and excessive salt intake are known as important risk factors for stomach cancer in humans. However, interactions of these two with gene expression profiles during gastric carcinogenesis remain unclear. In the present study, we investigated global associated prognosis human using a mouse model. Methods To find candidate genes involved carcinogenesis, firstly constructed carcinogen-induced tumor model combined high-salt diet. C57BL/6J...

Background Current definitions of overweight/obesity and central adiposity guidelines are based on Western populations, may not be appropriate for the Chinese population. More data among needed to address this issue. We aimed identify cut-offs body mass index (BMI) waist circumference that confer increased risk cardiovascular disease in a population Shanghai. Methods Results A representative, cross-sectional sample 13,817 adults aged >18 years was studied In men women, blood pressure...

Abstract Peritoneal adhesions are fibrous tissues formed after surgery. Both cytokines and transforming growth factors ( TGF s) involved in this process. The objective of study was to investigate the cross talk between these entities. drainage fluid surgery from patients rodent models examined by enzyme‐linked immunosorbent assay fluorescence‐activated cell sorter. Data showed that concentrations interferon IFN )‐γ interleukin IL )‐17 reached their peaks 6–12 hours surgery, whereas ‐β1 two...

Abstract Invariant NKT (iNKT) cells express markers of both T and NK may produce various cytokines to regulate liver immunity. However, the role iNKT in progression HBV‐relative cirrhosis (HBV‐LC) is incompletely understood. Here, we investigated impact peripheral on a cohort patients with HBV‐LC. The frequency, number, activation status, apoptosis proliferation ability were detected flow cytometry. hepatocyte cell line (MIHA) hepatic stellate (LX‐2) was cytometry PCR. In HBV‐LC patients,...

To evaluate the changes in body mass index (BMI) and waist circumference (WC) their associations with prevalence of hypertension type 2 diabetes mellitus (T2DM) Chinese adults.2 consecutive population-based cross-sectional surveys.A total 12 districts seven counties Shanghai, China.12 329 randomly selected participants survey 2002-2003, 7423 2009. All were residents Shanghai aged 35-74 years.Measured BMI WC. Previously diagnosed newly identified T2DM by measured blood pressure, fasting...

Glycosylated hemoglobin A1c (HbA1c) has been applied to identify type 2 diabetes (T2DM) in the U.S. and European countries. It not used China mainly due lack of a standardized approach measure HbA1c, short knowledge about racial-specific standard deficiency an optimal cut-off point.To evaluate combination HbA1c fasting plasma glucose (FPG) diagnosing T2DM Chinese adults, multistage sampling cross-sectional study was conducted Shanghai, China, 2009. The FPG measurement, assay, oral tolerance...

Purpose . Pancreatitis can lead to systemic inflammatory response, but the relationship between lymphocyte changes and patients with pancreatitis remains unclear. In this study, we evaluated feedback function of in peripheral subsets on condition pancreatitis. Materials Methods 131 acute (AP) 11 chronic (CP) constituted patients’ group; 20 healthy individuals were enrolled as controls (HC). Serum concentration C-reactive protein (CRP), amylase, lipase frequency absolute number many types...

Long non-coding RNAs (lncRNAs), as one common type of RNAs, play a critical role in the tumorigenesis and development hepatocellular carcinoma (HCC). In current study, we aimed to assess correlation between lncRNAs expression levels prognosis HCC patients. A lncRNA-based signature was also developed predict this work. The profiles tissues tumor para-carcinoma were obtained from Cancer Genome Atlas (TCGA) database. prognostic model established by least absolute shrinkage selection operator...

Brain natriuretic peptide (BNP) is used as a marker of cardiac dysfunction to predict heart failure mortality.The significance the prognostic ability BNP for liver cirrhosis remains unknown, although levels seen in are high.We aimed determine whether level related stage and could serve (predict 1-year all-cause mortality).We recruited 92 patients at different stages 81 controls matched by age gender this study.At admission, physical examination measurements were performed.Upon discharge, 89...

Abstract Peritoneal adhesion occurs frequently after gastrointestinal/gynecological surgery. Tissue repair and regeneration are very important during this process. IL‐22 is an cytokine that secreted from immune cells but functions on mesenchymal cells, such as mesothelial cells. The objective of study was to investigate the roles its regulators formation. Postsurgical peritoneal drainage fluid patients rodent models examined by enzyme‐linked immunosorbent assay fluorescence‐activated cell...

Abstract Background Many studies showed that the prognosis of hepatocellular carcinoma (HCC) was significantly associated with expressions TP53 and LRP1B. However, potential influence two genes on malignant progression HCC is still to be expounded. Methods According correlation analysis between immune cells expression levels LRP1B, we filtered perform unsupervised clustering analysis. Integration multi‐omic data identified genetic alteration epigenetic alteration. In addition, pathway used...

The uncertainty resulting from missing genotypes in low-coverage whole-genome sequencing (LCWGS) data complicates genotype imputation. aim of this study is to find out an optimal strategy for accurately imputing LCWGS and assess its effectiveness genomic prediction (GP) genome-wide association (GWAS) on economically important traits Large White pigs. 1 423 pigs were imputed using three different strategies: (1) the high-coverage (HCWGS) 30 key progenitors as reference panel (Ref_LG); (2)...

In this study, the NUCC-3 choriocarcinoma cell line was used as an in vitro placental model to investigate effects of follistatin on basal and GnRH-stimulated hCG secretion and/or its subunit mRNA levels. Follistatin (1.5-100 ng/ml; 48 h) alone did not affect or GnRH increased beta-subunit levels a dose-dependent manner, with maximal (2.37- 2.4-fold increases, respectively) at dose 10(-8) M after 24 h culture (P < 0.001). The time-course study showed that increase induced by occurred between...

Abstract Background Peri-implantitis (PI） is a common complication of dental implants that can result in implant failure. The study aims to identify genes related PI and Ferroptosis, analyze relevant molecules pathways, perform immune infiltration analysis, with the goal investigating classification creating diagnostic model using its genes. Methods first identified Ferroptosis-related (FRGs) by downloading datasets GSE33774 GSE57631 intersecting them FRGs set. Enrichment including Gene Set...

This study was to investigate the clinical features of chronic obstructive pulmonary disease (COPD) patients with concurrent acute myocardial infarction (AMI) and analyze occurrence in-hospital adverse events. Patients AMI who were admitted Beijing Chaoyang Hospital from January 2019 August 2023 retrospectively collected. All divided into COPD group simple according whether they COPD. received interventional treatment for AMI. The events compared between two groups. A total 183 aged...