A5100775196- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Vibrio bacteria research studies
- Circular RNAs in diseases
- Microbial infections and disease research
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer-related Molecular Pathways
- Genomics, phytochemicals, and oxidative stress
- Animal Virus Infections Studies
- RNA modifications and cancer
- interferon and immune responses
- Eicosanoids and Hypertension Pharmacology
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Traditional Chinese Medicine Analysis
- Advanced Glycation End Products research
- Hippo pathway signaling and YAP/TAZ
- Parasitic Infections and Diagnostics
- Mycobacterium research and diagnosis
- Cancer, Lipids, and Metabolism
- Polymer Science and PVC
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Beetle Biology and Toxicology Studies
Eighth Affiliated Hospital of Sun Yat-sen University
2025
Sun Yat-sen University
2020-2025
Hebei Medical University
2021-2023
Fourth Hospital of Hebei Medical University
2021-2023
Shanghai Jiao Tong University
2020-2022
Guangxi Medical University
2010-2021
First Affiliated Hospital of GuangXi Medical University
2010-2021
Beijing University of Chinese Medicine
2020-2021
Third Affiliated Hospital of Sun Yat-sen University
2020
Wenzhou Medical University
2017-2019
Many chemotherapeutic agents have been successfully used to treat hepatocellular carcinoma (HCC); however, the development of chemoresistance in liver cancer cells usually results a relapse and worsening prognosis. It has demonstrated that DNA methylation histone modification play crucial roles chemotherapy resistance. Currently, extensive research shown there is another potential mechanism gene expression control, which mediated through function short noncoding RNAs, especially for...
5-Fluorouracil (5-FU) has been widely applied to treat various types of cancers, including hepatocellular carcinoma (HCC). However, primary or acquired 5-FU resistance prevents the clinical application this drug in cancer therapy. Herein, our study is first demonstrate that lower expression KRAL, a long non-coding RNA (lncRNA), mediates HCC via miR-141/Keap1 axis. Cell proliferation assays, western blot analysis, qRT-PCR, dual-luciferase reporter assay and immunoprecipitation were performed...
Bacterial Rhs proteins containing toxic domains are often secreted by type VI secretion systems (T6SSs) through unclear mechanisms. Here, we show that the T6SS Rhs-family effector TseI of Aeromonas dhakensis is subject to self-cleavage at both N- and C-terminus, releasing middle core two VgrG-interacting (which name VIRN VIRC). VIRC an endonuclease, immunity protein TsiI protects against toxicity direct interaction. Proteolytic release mediated, respectively, internal aspartic protease...
The occurrence rate of liver cancer is increasing in recent years. significance lactylation tumor cells should not be neglected. This study aimed to discover a gene signature related that can used for diagnostic applications. Data were downloaded from Cancer Genome Atlas (TCGA), GSE14520, GSE84402, and GSE62232 datasets. Differential analysis random forest performed hepatocellular carcinoma (HCC). Receiver operating characteristic (ROC) curve was evaluate efficiency, logistic regression...
Homeobox B7 (HOXB7) has been identified associated with poor prognosis of hepatocellular carcinoma (HCC). However, the specific mechanism by which HOXB7 promotes malignant progression HCC remains to be determined.Immunohistochemistry (IHC) was used detect expression level in 77-paired tissue samples, and correlation between assessed. The location confirmed immunofluorescence. Cell Titer-Blue assay assess proliferation hepatoma cells. stem-like properties cells were analysed sphere formation...
We recently establish that aspafilioside B, a steroidal saponin extracted from Asparagus filicinus, is an active cytotoxic component. However, its antitumor activity till unknown. In this study, the anticancer effect of B against HCC cells and underlying mechanisms were investigated. Our results showed inhibited growth proliferation cell lines. Further study revealed could significantly induce G2 phase cycle arrest apoptosis, accompanying accumulation reactive oxygen species (ROS), but...
Thapsigargin (TG) is a natural product that exists in most parts of the plant Thapsia garganica L. and possesses potential anticancer activities against variety tumor cell lines. TG induces endoplasmic reticulum (ER) stress apoptosis by inhibiting cancer growth. However, antineoplastic effect human adrenocortical carcinoma (ACC) cells still unknown.In this study, two ACC lines including SW-13 NCI-H295R were employed to explore role ACC. A mouse xenograft model was established verify vivo....
Delivery of cargo proteins via protein secretion systems has been shown as a promising tool in various applications. However, are often used by pathogens to cause disease.
Gametocyte proteins of Eimeria (E.) spp. are important components the oocyst wall and some have been used to develop transmission-blocking vaccines against avian coccidiosis.Total RNA isolated from E. necatrix gametocytes was utilized as templates for RT-PCR amplification sequencing cDNA encoding a gametocyte protein using gene-specific primers. The cloned into bacterial expression vector pET28a(+) expressed in coli BL21 cells. antigenicity recombinant its localization different life-cycle...
The association between long non-coding RNA zinc finger antisense 1 (ZFAS1) and acute myeloid leukemia (AML) has not yet been investigated. present study aimed to assess the potential role of ZFAS1 in AML cell proliferation apoptosis. expression mRNA various lines (HL-60, KG-1, ML-1 SKNO-1) was measured by reverse transcription-quantitative polymerase chain reaction. results showed that increased all four human compared with control (T lymphocytic or Burkitt's lymphoma). Transfection small...
The unique geographical environment at high altitudes may cause a series of diseases, such as acute altitude reaction, cerebral edema, and pulmonary edema. Gymnadenia conopsea (L.) R. Br. has been reported to have an effect on high-altitude hypoxia. However, the molecular mechanism, especially expression long noncoding RNAs (lncRNAs), is not yet clear.The profiles lncRNAs in hypoxia-induced brain injury mice treated with by using microarray method.A total 226 differentially expressed...
Adrenocortical carcinoma (ACC) is an invasive tumor that occurs in the endocrine system. Increasing evidence has shown endoplasmic reticulum (ER) stress and autophagy play important role formation. Tauroursodeoxycholic acid (TUDCA) ER chemical chaperone can alleviate stress. In present study, TUDCA promoted proliferation, migration invasion of ACC SW‑13 NCI‑H295R cells. Reverse transcription‑quantitative PCR western blot analysis showed expression glucose‑regulated protein 78, a promoter...
Background: Neural stem cells (NSCs) are undifferentiated precursor that have the ability to self-renew and proliferate capacity become either glia (oligodendrocytes astrocytes) or neurons. NSCs can act as beneficial adjuncts for many neurological disorders, such cerebral infarction, spinal cord injuries, Alzheimer's disease, Parkinson's disease. Long noncoding RNAs (lncRNAs) play essential roles during cell differentiation, proliferation, metabolism. This study aimed explore role played by...
To study the effect of long non-coding RNAs (lncRNA) on aspirin-treated colon cancer cells.To changes lncRNA in cells by microarray analysis, real-time quantitative PCR (qPCR) was used to verify expression selected and mRNA. The effects aspirin proliferation metastasis siRNA transfected control were detected Cell Counting Kit-8 (CCK-8) Transwell experiments. Bioinformatics tools employed analyze potential function lncRNA.Compared with group, inhibited cells. Microarray analysis showed that a...
Abstract Pseudomonas aeruginosa is an important human pathogen that can cause severe wound and lung infections. It employs the type VI secretion system (H1-T6SS) as a molecular weapon to carry out unique dueling response deliver toxic effectors neighboring sister cells or other microbes after sensing external attack. However, underlying mechanism for such not fully understood. Here, we examined role of all H1-T6SS VgrG proteins in assembly signal by ectopic expression, combinatorial deletion...
The protein-kinase-R- (PKR-) like endoplasmic reticulum kinase (PERK) signaling pathway is a well-known promoter of cell apoptosis. In this study, we aimed to determine whether salubrinal (Sal), selective activator eukaryotic translation initiation factor 2 (eIF2α), can induce apoptosis human adrenocortical carcinoma (ACC) via activating the PERK/eIF2α/ATF4 pathway, and potential mechanisms action were explored. ACC lines, including SW-13 NCI–H295 R, used....