A5101421165- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Innovative Microfluidic and Catalytic Techniques Innovation
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Ferroptosis and cancer prognosis
- Autophagy in Disease and Therapy
- Semiconductor Lasers and Optical Devices
- Advanced biosensing and bioanalysis techniques
- Advanced Algorithms and Applications
- Cancer Cells and Metastasis
- Radio Frequency Integrated Circuit Design
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
- Circular RNAs in diseases
- Slime Mold and Myxomycetes Research
- Virus-based gene therapy research
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Geoscience and Mining Technology
- Photonic and Optical Devices
- Epigenetics and DNA Methylation
Affiliated Hospital of Hebei University
2022-2023
First Affiliated Hospital of Wenzhou Medical University
2018-2023
Wenzhou Medical University
2018-2023
Xi'an Jiaotong University
2010-2023
Beijing University of Technology
2023
Hebei University
2023
Zhengzhou University
2022
Second Affiliated Hospital of Zhejiang University
2022
Qingdao University
2019
Affiliated Hospital of Qingdao University
2019
Inefficient intracellular gene release and transfection limit nonviral delivery applications in cancer therapy. Reactive oxygen species (ROS) responsive is the most widely explored strategy for such applications, yet development of fast safe ROS nanocarriers proves to be a challenge because chemical equilibrium high glutathione levels. Here, we report an ultrasound-enhanced charge-reversal polymeric nanocarrier (BTIL) efficient pancreatic delivery. The BTIL composed B-PDEAEA/DNA...
Abstract Kidney cancer (KC) seriously impacts public health. We detected the function and mechanism of circular RNA C3P1 (circC3P1) in KC cells. CCK‐8, flow cytometry, migration, invasion assay were respectively used to investigate efficacies circC3P1 microRNA (miR)‐21 on cell viability, apoptosis, invasion. Phosphatase tensin homologue deleted chromosome 10 (PTEN), circC3P1, miR‐21 expression changed by transfection quantitative reverse‐transcription polymerase chain reaction. Moreover,...
Abstract Background Temozolomide (TMZ) treatment efficacy in glioblastoma (GBM) has been limited by resistance. The level of O-6-methylguanine-DNA methyltransferase (MGMT) and intrinsic DNA damage repair factors are important for the TMZ response patients. Here, we reported a novel compound, called EPIC-0307, that increased sensitivity inhibiting specific proteins MGMT expression. Methods EPIC-0307 was derived molecular docking screening. RNA immunoprecipitation (RIP), chromatin (ChIRP)...
Abstract Background Temozolomide (TMZ) treatment efficacy in glioblastoma is determined by various mechanisms such as TMZ efflux, autophagy, base excision repair (BER) pathway, and the level of O6-methylguanine-DNA methyltransferase (MGMT). Here, we reported a novel small-molecular inhibitor (SMI) EPIC-1042 (C20H28N6) with potential to decrease efflux promote PARP1 degradation via autolysosomes early stage. Methods was obtained from receptor-based virtual screening. Co-immunoprecipitation...
Glioblastoma (GBM) is the most common and lethal type of primary malignant central nervous system (CNS) tumor with an extremely poor prognosis, mesenchymal subtype GBM has worst prognosis. Here, we found that lncRNA PRADX was overexpressed in transcriptionally regulated by RUNX1-CBFβ complex, suppressed BLCAP expression via interacting EZH2 catalyzing trimethylation lysine 27 on histone H3 (H3K27me3). Moreover, showed interacted STAT3 reduced phosphorylation, activated promoted ACSL1...
The RNA contained in exosomes plays a crucial role information transfer between cells various life activities. accurate detection of low-abundance exosome (exRNA) is great significance for cell function studies and the early diagnosis diseases. However, their intrinsic properties, such as short length high sequence homology, represent challenges exRNA detection. In this paper, we developed dual-signal isothermal amplification method based on rolling circle (RCA) coupled with DNAzyme...
Growing evidences suggest that autophagy plays a momentous part in the tumorigenesis and development of hepatocellular carcinoma (HCC). However, there are not many researches to predict prognosis HCC using autophagy-related genes. Therefore, based on clinical information RNA-Seq data The Cancer Genome Atlas portal (TCGA), 13 autophagy‑related gene pairs were screened build signature by least absolute shrinkage selection operator (LASSO) regression analysis. Besides, International Consortium...
Long non-coding RNAs (lncRNAs), as one common type of RNAs, play a critical role in the tumorigenesis and development hepatocellular carcinoma (HCC). In current study, we aimed to assess correlation between lncRNAs expression levels prognosis HCC patients. A lncRNA-based signature was also developed predict this work. The profiles tissues tumor para-carcinoma were obtained from Cancer Genome Atlas (TCGA) database. prognostic model established by least absolute shrinkage selection operator...
Abstract Background Many studies showed that the prognosis of hepatocellular carcinoma (HCC) was significantly associated with expressions TP53 and LRP1B. However, potential influence two genes on malignant progression HCC is still to be expounded. Methods According correlation analysis between immune cells expression levels LRP1B, we filtered perform unsupervised clustering analysis. Integration multi‐omic data identified genetic alteration epigenetic alteration. In addition, pathway used...
N6-Methyladenosine (m6A) stands out as the predominant internal modification in mammalian RNA, exerting crucial regulatory functions metabolism of mRNA. Currently available methods have been limited by an inability to quantify m6A at precise sites. In this work, we screened a Bst 2.0 warm start DNA polymerase with capability discriminating from adenosine (A) and developed robust RNA detection method that enables isothermal ultrasensitive quantification single-base resolution. The limit assay...
Prostate cancer (PCa) is the most common male reproductive tract malignant tumor, accurate evaluation of PCa characterization and prognostic prediction at diagnosis are vital for effective administration disease, especially molecular level. In this study, 48 CpG sites with differential methylation associated overall survival (OS) were screened out between normal adjacent tissues. 16 selected by least absolute shrinkage selection operator (LASSO) risk score formula methylated-based classifier...
Backgroud: The prognosis of glioblastoma (GBM) is poor, and mesenchymal GBM has the worst prognosis. Here we aimed to elucidate transcription mechanism lncRNA PRADX its role in STAT3 signaling pathway energy metabolism activation GBM. Methods: TCGA CGGA databases were used analyze correlation between Western blotting, qRT-PCR, immunoprecipitation, dual-luciferase reporter assays performed determine molecular PRADX. Immunofluorescence (IF) co-immunoprecipitation (co-IP) conducted explore...