A5023994674- RNA Research and Splicing
- Retinal Development and Disorders
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Circular RNAs in diseases
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Kruppel-like factors research
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Glaucoma and retinal disorders
- Retinopathy of Prematurity Studies
- Axon Guidance and Neuronal Signaling
- Developmental Biology and Gene Regulation
Universidade Federal do Rio de Janeiro
2018-2025
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2024
TU Dresden
2019-2024
Berlin-Brandenburger Centrum für Regenerative Therapien
2022
Corticogenesis consists of a series synchronised events, including fate transition cortical progenitors, neuronal migration, specification and connectivity. NeuroD1, basic helix-loop-helix (bHLH) transcription factor (TF), contributes to all these but how it coordinates independently is still unknown. Here, we demonstrate that NeuroD1 expression accompanied by gain active chromatin at large number genomic loci. Interestingly, transcriptional activation loci relied on high local density...
Retinal ganglion cells (RGCs) are the projection neurons of retina, and their death promotes an irreversible blindness. Several factors were described to control genesis during retinal development which include Atoh7 as a major orchestrator for RGC program downstream targets, including Pou4f factors, in turn regulate key aspects terminal differentiation. The absence POU4F family genes results defects differentiation, aberrant axonal elaboration ultimately death, confirming requirement...
ABSTRACT Retinal ganglion cell (RGC) degeneration is a hallmark of glaucoma, the most prevalent cause irreversible blindness. Thus, therapeutic strategies are needed to protect and replace these projection neurons. One innovative approach promote de novo genesis RGCs via manipulation endogenous sources. Here, we demonstrate that pluripotency regulator gene Krüppel-like factor 4 (Klf4) sufficient change potency lineage-restricted retinal progenitor cells generate in vivo. Transcriptome...
MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over recent years, many studies have extensively characterized involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog cortical miRNAs expressed cell-specific manner progenitor types developing mammalian cortex is still missing. Overcoming this limitation, here we exploited double reporter mouse line...
ABSTRACT Retinal ganglion cells (RGCs) are the projection neurons of retina. In early retinal progenitor (RPCs), Atoh7 orchestrates developmental RGC program and regulates expression critical downstream targets, including Pou4f factors. The absence Pou4f2 or more POU4F family genes results in defects differentiation, aberrant axonal elaboration ultimately death, confirming requirement factors for development survival, with a role regulating axon outgrowth pathfinding. Here, we investigated...
Regulation of gene expression is critical for fate commitment stem and progenitor cells during tissue formation. In the context mammalian brain development, a plethora studies have described how changes in individual genes characterize cell types across ontogeny phylogeny. However, little attention has been paid to fact that different transcripts can arise from any given through alternative splicing (AS). Considered key mechanism expanding transcriptome diversity evolution, assessing full...
Smad anchor for receptor activation (SARA, zfyve9) has been classically observed in early endosomes of different cells types where it regulates vesicular transport proteins and membrane components. Very few other members the zinc finger FYVE domain-containing family (zfyve) have functions than controlling trafficking. By analyzing SARA localization throughout mouse embryonic brain development, we detected that besides endosomal also targets neuronal nuclei, specifically cortical layers V/VI....
ABSTRACT Regulation of gene expression is critical for fate commitment stem and progenitor cells during tissue formation. In the context mammalian brain development, a plethora studies have described how changes in individual genes characterize cell types across ontogeny phylogeny. However, little attention was paid to fact that different transcripts can arise from any given through alternative splicing (AS). Considered key mechanism expanding transcriptome diversity evolution, assessing...
Abstract Retinal ganglion cell (RGC) degeneration is a hallmark of glaucoma, the most prevalent cause irreversible blindness. Thus, innovative therapeutic strategies are needed to protect and replace these projection neurons. It has been shown that endogenous glial cells retina, Müller cells, can be directly reprogrammed into late-born retinal interneurons. However, since RGCs first neurons born during development, replacement damaged requires reprograming an early neurogenic state. Here, we...
ABSTRACT MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over past years, many studies have extensively characterized involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog cortical miRNAs cell-specifically expressed progenitor types developing mammalian cortex is still missing. Overcoming this limitation, here we exploited double reporter mouse...