Many Oriental people possess a liver mitochondrial aldehyde dehydrogenase where glutamate at position 487 has been replaced by lysine, and they have very low levels of activity. To investigate the cause lack activity this dehydrogenase, we mutated residue rat human to lysine expressed mutant native enzyme forms in Escherichia coli. Both recombinant dehydrogenases showed same molecular kinetic properties as isolated from mitochondria. The E487K mutants were found be active but possessed...

Objective The aim of this study was to investigate whether remedial hydration (RH) reduces the incidence contrast-induced nephropathy (CIN) and short-term adverse events in ST-elevation myocardial infarction (STEMI) patients undergoing primary percutaneous coronary intervention (PCI). Methods A total 216 consecutive STEMI were prospectively randomly assigned into two groups: 108 RH group no (control) group. serum creatinine (SCr) clearance (CCr) levels measured on admission at 24, 48 72...

Fosl2, a member of the AP-1 family, has been widely studied in fields tumorigenesis and immune response, but its role folliculogenesis remains unclear. In this investigation, we presented comprehensive vitro vivo evidence to precisely define biological functions Fosl2 folliculogenesis. both mouse sheep, demonstrated that knockdown effectively inhibited cell proliferation promoted apoptosis primary GCs cultured gonadotropin (GTH)-dependent follicles. To explore in-vivo function generated an...

ALI/ARDS is a severe lung injury leading to refractory respiratory failure, accounting for high morbidity and mortality. However, therapeutic approaches are rather limited. Targeting long non-coding RNA MALAT1 microRNA miR-181a-5p might be potential option intervention. We aimed investigate the role of MALAT in pathogenesis ALI/ARDS, test effects targeting intervention vitro. levels were measured plasma from patients. In vitro human pulmonary microvascular endothelial cell (HPMEC) was...

To investigate the role of miRNA-15b in cardiomyocyte apoptosis after ischemia reperfusion injury acute myocardial infarction (AMI), we conducted AMI rat model by using left anterior descending ligation and performed hypoxia/reoxygenation experiments H9c2 cells. MiRNA-15b was measured quantitative reverse transcription PCR (qRT-PCR). Cardiomyocyte determined terminal deoxynucleotide transferase dUTP nick end labeling staining. Synthesized mimic inhibitor were transfected into cells...

Introduction: SK channels have functional importance in the cardiac atrium of many species, including humans. Pharmacological blockage has been reported to be antiarrhythmic animal models atrial fibrillation; however, exact mechanism channel inhibition remains unclear. Objectives: We speculated that together with a direct repolarizing current, previously observed depolarization resting membrane potential (RMP) after reduces sodium availability, thereby prolonging effective refractory period...

Although particulate matter, with diameters < 2.5 µm (PM2.5) and 10 (PM10), other pollutants have been associated cardiovascular morbidity mortality, the effect of on acute myocardial infarctions (AMIs) has rarely investigated in Asia, especially Shanghai, China.Between 1 November 2013 27 April 2014, 972 patients from Pudong District, Shanghai City, were assessed by Emergency Medical Service. A case-crossover design was used to analyze exposure air pollution AMI risk. Exposures PM2.5, PM10,...

The current study aimed to investigate the relevance of gap junction protein connexin Cx43 in coronary artery smooth muscle cell (SMC) heterogeneity and restenosis. SMCs were isolated from 3‑month‑old pigs using enzymatic digestion. Two distinct SMC populations isolated: Rhomboid (R) spindle‑shaped (S) cells. S‑SMCs exhibited relatively lower rates proliferation, exhibiting a classic ''hills‑and valleys'' growth pattern; R‑SMCs displayed increased proliferation rates, growing as mono‑ or...

microRNA-3568 (miR-3568) has been reported to be associated with atherosclerosis. Only few data describe the expression and underlying mechanism of miR-3568 in regulating cardiac ischemia-reperfusion (I/R) injury such as apoptosis. In this study, we therefore sought investigate potential function simulated I/R-induced apoptosis H9C2 cardiomyocytes related signaling pathways involved. Flow cytometry was performed examine cell The miR-3568, Survivin, Bcl-2, ERK, JNK, p38, AKT, STAT3 measured...

In some circumstances, a high degree of sedation that results in child being unconscious at the time parental separation is desirable. We set out to investigate efficacy and safety rectal premedication regimen designed produce this increased level sedation. Sixty-seven children aged two 24 months were randomised into groups. Group MK received 4 mg.kg -1 ketamine, 0.5 midazolam 0.02 atropine group MKK 8 per rectum. The score 30 minutes after drug administration response intravenous...

In a previous study, we found that titrating clopidogrel maintenance doses (MDs) according to vasodilator-stimulated phosphoprotein (VASP) monitoring minimised the rate of major adverse cardiovascular and cerebral events (MACCE) after percutaneous coronary intervention (PCI) without increasing bleeding in patients with high on-treatment platelet reaction clopidogrel. This study aimed investigate whether VASP-guided MD could reduce thromboembolism atrial fibrillation (AF) requiring...

The aim of the present study was to explore infection route ventilator-associated pneumonia (VAP) and assess effectiveness a combined nursing strategy prevent VAP in intensive care units. Bacteria from gastric juice drainage hypolarynx lower respiratory tracts patients with were analyzed using genome macrorestriction-pulsed-field gel electrophoresis (GM-PFGE). A total 124 tracheal intubation placed intervention group treated strategy, comprising mosapride (gastric motility stimulant)...

Objective To observe the effects of different posture on plasma ANP and AngⅡ during laparoscopy.Methods Forty patients,ASA class I or Ⅱ,scheduled for elective laparoscopy surgery under general anesthesia were included in this study.The patients randomly divided into two groups according to their laparoscopy(20 cases each group).The group A arranged a head-down tilt position B head-up requirement operation.Blood samples taken from central venous at four time points before...

Objective To observe the incidence of cardiac arrhythmia in patients with acute ischemic stroke.Methods Dynamic electrocardiogram recordings were performed 84 stroke and to analyze relationship between rate types stroke.Results Total detection various was 78.5%.Conclusions Incidence very high should be highly attached importance to.Therapy primary disease、dynamic recordings,electrocardiographic monitoring conventionally,strengthening diagnosis therapy simultaneous. Key words: Ischemic...

Objective To observe the effects of different exposure times under pulsed radiofrequency (PRF) on thermal withdrawal latency (TWL) and mechanical threshold (MWT) in rat model with chronic constriction injury (CCI). Methods Seventy-two adult male SD rats were randomly divided into 4 groups: sham group, PRF-120 s PRF-480 group PRF-720 18 each group. The only placed RF needle without pulse intervention, while other groups treated PRF intervention (2 Hz, 55 V) after CCI was successfully...

Objective To explore the characteristics of autonomic nervous activity in patients with vasovagal syncope detected by head-up tilt test. Methods 47 were selected from our hospital June 2018 to August 2019. The basic test and nitroglycerin-induced conducted divided into positive group negative group. 12 lead electrocardiogram dynamic recorded during tilting process. Blood pressure was measured every 5 minutes. Frequency domain indices heart rate variability (LFnorm, HFnorm, LF/HF)...

Abstract Background Long non-coding RNA metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) plays an important role in the pathophysiological process of inflammation. We aimed to investigate MALAT1 and its function modulating miRNA-181a-3p Bcl-2 lipopolysaccharide (LPS)-induced acute injury (ALI). Methods analysed ALI patients, as well alveolar epithelial cell models LPS-induced injury. The expression were evaluated by qRT-PCR, was measured western blot. Inflammatory factors...