A5036496290- Cancer, Lipids, and Metabolism
- Medicinal Plant Extracts Effects
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Drug Transport and Resistance Mechanisms
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Trace Elements in Health
- Bladder and Urothelial Cancer Treatments
- Potato Plant Research
- Metabolism, Diabetes, and Cancer
- 14-3-3 protein interactions
- Phytoestrogen effects and research
- Silymarin and Mushroom Poisoning
- RNA modifications and cancer
- Medicinal Plants and Bioactive Compounds
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Cancer Immunotherapy and Biomarkers
- Sarcoma Diagnosis and Treatment
- Estrogen and related hormone effects
- Angiogenesis and VEGF in Cancer
- Antioxidant Activity and Oxidative Stress
Harbin Medical University
2021-2025
Fourth Affiliated Hospital of Harbin Medical University
2023-2025
University of California, Irvine
2015-2024
University of California, Irvine Medical Center
2012-2024
VA Long Beach Healthcare System
2013-2024
Community Cancer Center
2022
Third Affiliated Hospital of Harbin Medical University
2021
UC Irvine Health
2013-2019
Southwest Medical University
2019
Montefiore Medical Center
2017
Background: Trastuzumab (Herceptin), an anti-HER2/neu receptor monoclonal antibody that inhibits growth of ErbB2-overexpressing breast cancer, is used to treat such cancers. Development resistance trastuzumab, however, common. We investigated whether insulin-like factor-I (IGF-I), which activates cell survival signals, interferes with the growth-inhibitory action trastuzumab. Methods: MCF-7/HER2-18 and SKBR3 human cancer models were assess proliferation, colony formation in soft agar, cycle...
Lysine specific demethylase 1 (LSD1), the first identified histone demethylase, plays an important role in epigenetic regulation of gene activation and repression. The up-regulated LSD1's expression has been reported several malignant tumors. In current study, we designed synthesized five series 1,2,3-triazole-dithiocarbamate hybrids screened their inhibitory activity toward LSD1. We found that some these compounds, especially compound 26, exhibited most robust inhibition Interestingly, 26...
Reduction in serum prostate-specific antigen (PSA) levels has been proposed as an endpoint biomarker for hormone-refractory human prostate cancer intervention. We examined whether a flavonoid antioxidant silibinin (an active constituent of milk thistle) decreases PSA carcinoma LNCaP cells and this effect biological relevance. Silibinin treatment grown resulted significant decrease both intracellular secreted forms concomitant with highly to complete inhibition cell growth via G 1 arrest...
Abstract The clinical usefulness of trastuzumab (Herceptin; Genentech, San Francisco, CA) in breast cancer treatment is limited by the rapid development resistance. We previously reported that IGF‐I signaling confers resistance to growth‐inhibitory actions a model system, but underlying molecular mechanism remains unknown. used SKBR3/neo cells (expressing few receptors) and SKBR3/IGF‐IR (overexpressing receptor) as our experimental model. antagonized trastuzumab‐induced increase level Cdk...
Abstract The ability of Frzb/secreted Frizzled-related protein 3 (sFRP3) to inhibit Wnt signaling and the localization Frzb/sFRP3 on chromosome 2q a region frequently deleted in cancers have led some investigators hypothesize that is tumor suppressor gene. Here, we examined biological effects an androgen-independent prostate cancer cell model. We showed expression PC-3 cells resulted decreased colony formation soft agar dramatic inhibition growth xenograft mouse When cellular morphology was...
Abstract Consumption of the traditional kava preparation was reported to correlate with low and uncustomary gender ratios (more cancer in women than men) incidences three kava-drinking countries: Fiji, Vanuatu, Western Samoa. We have identified flavokawain A, B, C but not major kavalactone, kawain, extracts as causing strong antiproliferative apoptotic effect human bladder cells. Flavokawain A results a significant loss mitochondrial membrane potential release cytochrome c into cytosol an...
Abstract Background Aberrations in the Wnt pathway have been reported to be involved metastasis of prostate cancer (PCa) bone. We investigated effect and underlying mechanism a naturally-occurring inhibitor, WIF1, on growth cellular invasiveness bone metastatic PCa cell line, PC3. Results The WIF1 gene promoter was hypermethylated its expression down-regulated majority (7 8) lines. Restoration PC-3 cells resulted decreased motility via up-regulation epithelial markers (E-cadherin, Keratin-8...
Abstract It has been reported that the progression of osteosarcoma was closely associated with aberrant activation canonical Wnt signaling. inhibitory factor-1 (WIF-1) is a secreted inhibitor whose role in human remains unknown. In this study, WIF-1 expression NHOst and cell lines determined by real-time reverse transcription-PCR, methylation-specific PCR, Western blotting analysis. addition, tissue array from patient samples examined for immunohistochemistry. Compared normal osteoblasts,...
Mediator kinases CDK19 and CDK8, pleiotropic regulators of transcriptional reprogramming, are differentially regulated by androgen signaling but both upregulated in castration-resistant prostate cancer (CRPC). Genetic or pharmacological inhibition CDK8 reverses the phenotype restores sensitivity CRPC xenografts to deprivation vivo. Prolonged CDK8/19 inhibitor treatment combined with castration not only suppresses growth also induces tumor regression cures. Transcriptomic analysis revealed...
Abstract We previously reported the Wnt receptor low‐density lipoprotein receptor‐related protein 5 (LRP5) was frequently expressed in osteosarcoma (OS) tissue and correlated with metastasis a lower disease‐free survival. Subsequent vitro analysis revealed that dominant‐negative, soluble LRP5 (sLRP5) can reduce cellular invasion. In current study, we examined molecular mechanisms of blocking canonical signaling by sLRP5 Saos‐2 cells. Transfection caused marked up‐regulation E‐cadherin this...
The incidence of human urinary bladder cancer increases markedly with age, suggesting a mechanistic connection between aging and carcinogenesis potential use anti-aging agents in chemoprevention. Rhodiola rosea, growing high altitude or cold regions the world, has been reported to have effects Drosophila. We demonstrated that R. rosea extract one its bioactive components, salidroside, inhibited growth cell lines minimal effect on nonmalignant epithelial cells TEU-2. Interestingly,...
Abstract Epigenetic silencing of secreted wingless-type (Wnt) antagonists through hypermethylation is associated with tobacco smoking and invasive bladder cancer. The Wnt inhibitory factor-1 (WIF1) has shown consistent growth-inhibitory effect on various cancer cell lines. Therefore, we assessed the mechanisms action WIF1 by either restoring expression in lines (T24 TSU-PR1) or using a recombinant protein containing functional domain. Both ectopic treatment domain resulted growth inhibition...
Limited success has been achieved in extending the survival of patients with metastatic and hormone-refractory prostate cancer (HRPC). There is a strong need for novel agents treatment prevention HRPC. We have shown that flavokawain B (FKB), kava chalcone, about 4- to 12-fold more effective reducing cell viabilities androgen receptor (AR)-negative, HRPC lines DU145 PC-3 than AR-positive, hormone-sensitive LAPC4 LNCaP, minimal effect on normal prostatic epithelial stromal cells. FKB induces...
Docetaxel is currently the most effective drug for treatment of castration-resistant prostate cancer (CRPC), but it only extends life by an average 2 months. Lycopene, antioxidant phytochemical, has antitumor activity against (PCa) in several models and generally safe. We present data on interaction between docetaxel lycopene CRPC models. The growth-inhibitory effect PCa cell lines was positively associated with insulin-like growth factor I receptor (IGF-IR) levels. In addition, enhanced...
Osteosarcoma (OS) is the most common primary bone malignancy with a high propensity for local invasion and distant metastasis. Limited by severe toxicity of conventional agents, therapeutic bottleneck osteosarcoma still remains unconquered. Flavokawain B (FKB), kava extract, has been reported to have significant anti-tumor effects on several carcinoma cell lines both in vitro vivo. Its efficacy low profile make FKB promising agent use as novel chemotherapeutic agent.In current study, we...
Purpose Colon cancer (CC) is a serious disease burden. The prognosis of patients with CC different, so looking for effective biomarkers to predict vitally important. Ferroptosis promising therapeutic and diagnosis strategy in CC. However, the role ferroptosis has not been studied. aim study build model related ferroptosis, provide clues further therapy Methods RNA-seq data were from TCGA (training group) GEO (testing group). R language Perl used process analyze data. LASSO regression...
Abstract Osteosarcoma(OS) is a highly aggressive bone cancer for which treatment has remained essentially unchanged decades. Although OS characterized by extensive genomic heterogeneity and instability, RB1 TP53 have been shown to be the most commonly inactivated tumor suppressors in OS. We previously generated mouse model with double knockout (DKO) of Rb1 Trp53 within cells osteoblastic lineage, largely recapitulates human nearly complete penetrance. SKP2 repression target pRb serves as...