A5100681768- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Virus-based gene therapy research
- Research in Cotton Cultivation
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Natural product bioactivities and synthesis
- Plant tissue culture and regeneration
- Circular RNAs in diseases
- Parasites and Host Interactions
- Molecular Biology Techniques and Applications
- Trace Elements in Health
- CRISPR and Genetic Engineering
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Insect Resistance and Genetics
- MicroRNA in disease regulation
Dalian Medical University
2025
Chongqing Medical University
2025
Second Affiliated Hospital of Chongqing Medical University
2025
Jilin University
2017-2024
The First Affiliated Hospital, Sun Yat-sen University
2021-2024
Sun Yat-sen University
2002-2024
Key Laboratory of Guangdong Province
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2000-2024
Chinese Academy of Fishery Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Ferroptosis is a novel cell death mechanism that mediated by iron-dependent lipid peroxidation. It may be involved in atherosclerosis development. Products of phospholipid oxidation play key role atherosclerosis. 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC) product present atherosclerotic lesions. remains unclear whether PGPC causes inducing endothelial ferroptosis. In this study, human umbilical vein cells (HUVECs) were treated with PGPC. Intracellular levels ferrous iron,...
The genes encoding β-actin (ACTB in human or Actb mouse) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH Gapdh are the two most commonly used references for sample normalization determination of mRNA level interested by reverse transcription (RT) ensuing polymerase chain reactions (PCR). In this study, bioinformatic analyses revealed that ACTB, Actb, GAPDH had 64, 69, 67 197 pseudogenes (PGs), respectively, corresponding genome. Most these PGs intronless similar size to authentic mRNA....
Abstract Although the routes of de novo pyridoxal 5′-phosphate (PLP) biosynthesis have been well described, studies engineering an intracellular PLP supply are limited and effects cellular levels on PLP-dependent enzyme-based whole-cell biocatalyst activity not described. To investigate cofactor availability biocatalysis, ribose 5-phosphate (R5P)-dependent pathway genes pdxS pdxT Bacillus subtilis were introduced into lysine decarboxylase (CadA)-overexpressing Escherichia coli strain...
Genetically identical female honeybee larvae with different diets develop into sterile workers or fertile queens. It remains unknown whether the reversible RNA N6-methyladenosine (m6A) mark functionally impact this "caste differentiation." Here, we profile transcriptome-wide m6A methylome of queen and worker at three instar stages discover that methylation dynamics are altered by differential feeding. Multiple comparisons show an obvious increase in marks during larval development reveal a...
New Delhi metallo-β-lactamase 1 (NDM-1), which is associated with resistance to carbapenem, was first reported in 2008. A sensitive and rapid molecular assay detect the plasmid bla(NDM-1) clinical isolates needed control its spread. We describe a loop-mediated isothermal amplification (LAMP) for detection of from pure culture sputum, urine, fecal samples. Eight sets primers were designed recognize six or eight distinct sequences on target bla(NDM-1), one set selected as most appropriate...
Shigella species possess a type III secretion system (T3SS), which is required for human infection and that delivers effector proteins into target host cells. Here, we show the effector, IpaH4.5 dampens pro-inflammatory cytokine response. In both Sereny test murine lung model, ΔipaH4.5 mutant strain caused more severe inflammatory responses significantly induced higher levels (MIP-2 TNF-α) in homogenates of wild type-infected mice. Moreover, there was threefold decrease bacterial...
N6-methyladenosine (m6A) is a ubiquitous reversible epigenetic RNA modification that plays an important role in the regulation of post-transcriptional protein coding gene expression. Liver vital organ and major metabolism with numerous functions. Information concerning dynamic patterns mRNA m6A methylation during postnatal development liver has been long overdue elucidation this information will benefit for further deciphering multitude functional outcomes methylation. Here, we profile...
The clinical application of advanced next-generation sequencing technologies is increasingly uncovering novel classes mutations that may serve as potential targets for precision medicine therapeutics. Here, we show a deep intronic splice defect in the COL6A1 gene, originally discovered by applying muscle RNA patients with findings collagen VI–related dystrophy (COL6-RD), inserts an in-frame pseudoexon into mRNA, encodes mutant α1(VI) protein exerts dominant-negative effect on VI matrix...
Hematopoietic stem cells (HSCs) are susceptible to replication stress, which is a major contributor HSC defects in Fanconi anemia (FA). Here, we report that HSCs relax the global chromatin by downregulating expression of architectural protein, DEK, response stress. DEK abnormally accumulated bone marrow (BM) CD34+ from patients with FA and Fancd2-deficient HSCs. haploinsufficiency promotes relaxation, stress relief, function recovery Furthermore, inhibition restores proliferation vitro...
Amplification of 19q is a frequent genetic alteration in many solid tumors, and SEI1 candidate oncogene within the amplified region. Our previous study found that oncogenic function was associated with chromosome instability. In this study, we report novel mechanism genomic instability involving SEI1-SET-NM23H1 pathway. Overexpression observed 57 100 esophageal squamous cell carcinoma cases. Functional showed had strong tumorigenic ability, overexpression could induce by increasing...
Coal gangue is a byproduct of coal mining and processing, according to incomplete statistics, China has amassed substantial stockpile exceeding 2600 large mountains, which poses serious threat the ecological environment. Utilizing as coarse aggregate produce concrete (GC) presents promising avenue for addressing disposal gangue; however, several challenges that need be tackled, such low strength poor resistance repeated loads. In this study, polypropylene fibers (PPFs) were incorporated into...
Diabetes mellitus of type 2 (T2D), also known as noninsulin-dependent diabetes (NIDDM) or adult-onset diabetes, is a common disease. It estimated that more than 300 million people worldwide suffer from T2D. In this study, we investigated the T2D, pre-diabetic and healthy human (no diabetes) bloodstream samples using genomic, genealogical, phonemic information. We identified differentially expressed genes pathways. The study has provided deeper insights into development useful information for...
Human lysozyme is a natural non-specific immune factor in human milk that plays an important role the defense of breastfed infants against pathogen infection. Although abundant milk, there only trace quantities pig milk. Here, we successfully generated transgenic cloned pigs with expression vector pBAC-hLF-hLZ-Neo and their first generation hybrids (F1). The highest concentration recombinant (rhLZ) vitro bioactivity was 2759.6 ± 265.0 mg/L F0 sows. Compared wild-type rhLZ inhibited growth...
Human RPE65 mutations cause a spectrum of retinal dystrophies that result in blindness. While have been almost invariably recessively inherited, c.1430A>G (p.(D477G)) mutation has reported to autosomal dominant retinitis pigmentosa (adRP). To study the pathogenesis this human mutation, we replicated knock-in (KI) mouse model using CRISPR/Cas9-mediated genome editing. Significantly, contrast patients, heterozygous KI mice do not exhibit any phenotypes visual function tests. When raised...
Abstract The potential pathogenicity of two homoplasmic mtDNA point mutations, 9035T>C and 4452T>C, found in a family afflicted with maternally transmitted cognitive developmental delay, learning disability, progressive ataxia was evaluated using transmitochondrial cybrids. We confirmed that the 4452T>C transition tRNA Met represented polymorphism; however, conversion ATP6 gene responsible for defective F 0 ‐ATPase. Accordingly, mutant cybrids had reduced oligomycin‐sensitive ATP...
Abstract Objectives Targeted delivery of mitoxantrone (MTO, an anthraquinone drug with high antitumour effect) may be achieved using a novel nanoparticulate system via binding the oestrogen receptor (ER, highly expressed in variety human tumours). Methods A liposomal nanoparticle (NP) was developed conjugate derived from 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (DSPE-PEG2000-NH2) and oestrone (ES, is known to bind ER) produce ES-targeted PEGylated...
Livin is a novel member of the inhibitor apoptosis protein (IAP) family that has been reported to be overexpressed in variety human malignancies, including osteosarcoma. However, potential roles tumorigenesis have not elucidated. In present study, we employed RNA interference (RNAi) technology suppress endogenous expression osteosarcoma cells and successfully generated U2-OS cell line with stably knockdown Livin. Functional analysis showed significantly reduced proliferation, colony...