A5029044945- Glioma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Sarcoma Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- CNS Lymphoma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- 3D Printing in Biomedical Research
- Genetics and Neurodevelopmental Disorders
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- Neuroblastoma Research and Treatments
University Medical Center Hamburg-Eppendorf
2022-2025
Universität Hamburg
2022-2025
Kinderkrebs-Zentrum Hamburg
2022-2024
Tel Aviv University
2020
Heidelberg University
2020
Abstract Background Molecular brain tumor diagnosis is usually dependent on tissue biopsies or resections. This can pose several risks associated with anesthesia neurosurgery, especially for lesions in the stem other difficult-to-reach anatomical sites. Apart from initial diagnosis, progression, recurrence, acquisition of novel genetic alterations only be proven by re-biopsies. Methods We employed Nanopore sequencing cell-free DNA (cfDNA) cerebrospinal fluid (CSF) and analyzed copy number...
Abstract Although the intraoperative molecular diagnosis of approximately 100 known brain tumor entities described to date has been a goal neuropathology for past decade, achieving this within clinically relevant timeframe under 1 h after biopsy collection remains elusive. Advances in third-generation sequencing have brought closer, but established machine learning techniques rely on computationally intensive methods, making them impractical live diagnostic workflows clinical applications....
Pediatric high-grade gliomas of the subclass MYCN (HGG-MYCN) are highly aggressive tumors frequently carrying amplifications, TP53 mutations, or both alterations. Due to their rarity, such have only recently been identified as a distinct entity, and biological well clinical characteristics not addressed specifically. To gain insights into tumorigenesis molecular profiles these tumors, ultimately suggest alternative treatment options, we generated genetically engineered mouse model by...
Abstract For exact molecular diagnosis of CNS tumors, tumor tissue obtained through surgery is usually needed. However, knowledge on the type beforehand might influence surgical strategies and reduce surgery-associated risks. Molecular analyses liquid biopsies have therefore gained increasing interest in order to secure before or replace rare instances. Moreover, minimally invasive nature enables a tight longitudinal monitoring with potentially higher sensitivity than imaging approaches. In...
Abstract Pediatric glioma of the subclass MYCN are highly aggressive tumors with a median age at diagnosis 8 years and survival only 14 months. Molecularly, they frequently carry amplifications, TP53 mutations or both these alterations. Due to their rarity, such have recently been identified as distinct entity, biological well clinical details not fully addressed. In order further investigate biology treatment options tumors, preclinical models urgently needed. Therefore, we generated...
Abstract Multiple recent publications have described a highly aggressive subgroup of pediatric glioblastoma, which is clearly separable from other and adult glioblastoma based on its DNA methylation profile (GBM MYCN). These tumors almost exclusively occur in children median overall survival only 14 months. Many this group are driven by MYCN amplifications harbor TP53 mutations. Otherwise, information about these still sparse treatment ineffective causes severe side effects many cases. In...