This study was planned to evaluate the efficacy of silver nitrate and gentamicin in treatment burn wound infection compare it with phage therapy using an isolated well-characterized Klebsiella-specific phage, Kpn5. A full-thickness induced mice infected Klebsiella pneumoniae B5055 via topical route. Different concentrations or were applied topically daily after establishment infection. Phage Kpn5 mixed hydrogel also at m.o.i. 200 on site. The these antimicrobial agents assessed basis...

Phage therapy has been a centre of attraction for biomedical scientists to treat infections caused by drug resistant strains. However, ability phage act only on extracellular bacteria and probability interference anti-phage antibodies in vivo is considered as important limitation bacteriophage therapy. To overcome these hurdles, liposome were used delivery vehicle this study. Anti-phage raised mice pooled serum was evaluated its neutralize free entrapped phage. Further, liposome-entrapped...

Staphylococcus comprises up to two-thirds of all pathogens in orthopaedic implant infections with two species respectively aureus and epidermidis, being the predominate etiological agents isolated. Further, emergence methicillin-resistant S. (MRSA), treatment has become more difficult, thus representing a devastating complication. Use local delivery system consisting S.aureus specific phage along linezolid (incorporated biopolymer) allowing gradual release at site represents new, still...

Pseudomonas aeruginosa is an opportunistic pathogen that colonizes urinary catheters, forms biofilms, and responsible for causing persistent recurrent nosocomial catheter-associated tract infections (UTIs). These show increased morbidity mortality in immunocompromised patients. Quorum sensing P. plays a key role biofilm formation, virulence factor production antimicrobial resistance. Because of emerging resistance aeruginosa, there need to find alternate nonantibiotic agent the control...

The in vitro efficacy of lytic bacteriophage as a therapeutic agent against Klebsiella pneumoniae biofilm, alone or combination with ciprofloxacin, was studied. pathogenic potential the resistant variants formed during treatment evaluated. A ciprofloxacin used for K. B5055 biofilm. and frequency variant formation were estimated after respective treatments. characterized their virulence potential. Bacteriophage able to eradicate biofilm effectively no significant difference observed its...

Pseudomonas aeruginosa , an opportunistic pathogen, is the third most common pathogen associated with nosocomial urinary tract infections (UTIs). The virulence of this organism due to its ability produce quorum-sensing (QS) signal molecules and form biofilms. These biofilms are usually resistant conventional antibiotics host immune responses. Recently, beneficial effects macrolides, especially azithromycin (AZM), have been shown in patients suffering from chronic caused by P. aeruginosa....

Bacteria have evolved multiple mechanisms, such as biofilm formation, to thwart antibiotic action. Yet antibiotics remain the drug of choice against clinical infections. It has been documented that young Klebsiella pneumoniae could be eradicated significantly by ciprofloxacin treatment alone. Since age is a decisive factor in determining outcome treatment, present study K. pneumoniae, grown for extended periods was treated with and/or depolymerase producing lytic bacteriophage (KPO1K2). The...

This study examined the therapeutic and prophylactic potential of bacteriophages in a mouse model Klebsiella pneumoniae lobar pneumonia. Phages were administered intraperitoneally. Liposome-entrapped phages (LP) effective treating infection, even when therapy was delayed by 3 days after induction In contrast, nonliposomal provided protection 24 hours infection. Administration 6 prior to intranasal bacterial challenge resulted complete protection, compared with LP, which 48 Increased...

Infections of bone and joint tissues following arthroplasty surgeries remain a major challenge in orthopaedic settings. Methicillin resistant Staphylococcus aureus (MRSA) is recognised as an established pathogen such infections. Combination therapy using linezolid bacteriophage impregnated biopolymer was investigated the present study alternative strategy to prevent MRSA colonisation on implant surface.Coating stainless steel grade K-wires achieved hydroxypropylmethlycellulose (HPMC) mixed...

The quorum sensing (QS) circuitry of Pseudomonas aeruginosa represents an attractive target to attenuate bacterial virulence and antibiotic resistance. In this context, phytochemicals harboring anti-virulent properties have emerged as alternative medicine combat pseudomonal infections. Hence, study was undertaken investigate the synergistic effects quenching (QQ) potential cinnamaldehyde (CiNN) in combination with gentamicin (GeN) against P. . QQ activity novel evaluated using a QS reporter...

Present-day healthcare employs several types of invasive devices, including urinary catheters, to improve medical wellness, the clinical outcome disease, and quality patient life. Among Foley catheter is most commonly used in patients for bladder drainage collection urine. Although such devices are very useful who cannot empty their various reasons, they also expose catheter-associated tract infections (CAUTIs). Catheter provides an ideal surface bacterial colonization biofilm formation,...