Klebsiella pneumoniae causes infections in humans especially immunocompromised patients. About 80 % of nosocomial caused by K. are due to multidrug-resistant strains. The emergence antibiotic-resistant bacterial strains necessitates the exploration alternative antibacterial therapies, which led our group study ability viruses (known as bacteriophages or simply phages) treat mice challenged with pneumoniae. Phage SS specific for B5055 was isolated and characterized, its potential a...

Staphylococcus aureus remains the predominant pathogen in diabetic foot infections and prevalence of methicillin resistant S.aureus (MRSA) strains further complicates situation. The incidence MRSA infected ulcers is 15-30% there an alarming trend for its increase many countries. Diabetes acts as immunosuppressive state decreasing overall immune functioning body to worsen situation, wounds inflicted with drug represent a morbid combination patients. Foot caused by are associated increased...

This study was planned to evaluate the efficacy of silver nitrate and gentamicin in treatment burn wound infection compare it with phage therapy using an isolated well-characterized Klebsiella-specific phage, Kpn5. A full-thickness induced mice infected Klebsiella pneumoniae B5055 via topical route. Different concentrations or were applied topically daily after establishment infection. Phage Kpn5 mixed hydrogel also at m.o.i. 200 on site. The these antimicrobial agents assessed basis...

Diabetic populations are more prone to developing wound infections which results in poor and delayed healing. Infection with drug resistant organisms further worsen the situation, driving searches for alternative treatment approaches such as phage therapy. Major drawback of therapy, however, is low persistence situ, suggesting refinement approach. In present work we address this issue by employing liposomes delivery vehicles. A liposome entrapped cocktail was evaluated its ability resolve a...

Phage therapy has been a centre of attraction for biomedical scientists to treat infections caused by drug resistant strains. However, ability phage act only on extracellular bacteria and probability interference anti-phage antibodies in vivo is considered as important limitation bacteriophage therapy. To overcome these hurdles, liposome were used delivery vehicle this study. Anti-phage raised mice pooled serum was evaluated its neutralize free entrapped phage. Further, liposome-entrapped...

Staphylococcus comprises up to two-thirds of all pathogens in orthopaedic implant infections with two species respectively aureus and epidermidis, being the predominate etiological agents isolated. Further, emergence methicillin-resistant S. (MRSA), treatment has become more difficult, thus representing a devastating complication. Use local delivery system consisting S.aureus specific phage along linezolid (incorporated biopolymer) allowing gradual release at site represents new, still...

The in vitro efficacy of lytic bacteriophage as a therapeutic agent against Klebsiella pneumoniae biofilm, alone or combination with ciprofloxacin, was studied. pathogenic potential the resistant variants formed during treatment evaluated. A ciprofloxacin used for K. B5055 biofilm. and frequency variant formation were estimated after respective treatments. characterized their virulence potential. Bacteriophage able to eradicate biofilm effectively no significant difference observed its...

Bacteria have evolved multiple mechanisms, such as biofilm formation, to thwart antibiotic action. Yet antibiotics remain the drug of choice against clinical infections. It has been documented that young Klebsiella pneumoniae could be eradicated significantly by ciprofloxacin treatment alone. Since age is a decisive factor in determining outcome treatment, present study K. pneumoniae, grown for extended periods was treated with and/or depolymerase producing lytic bacteriophage (KPO1K2). The...

Success of biofilm dwelling bacteria in causing persistent and chronic infections is attributed to their resistance towards antibiotics immune defences. Free iron critical for the growth associated bacteria. Therefore present study, effect limiting levels by addition divalent Co[II] ions combination with a bacteriophage was used preventing/disrupting Klebsiella pneumoniae biofilms. A significantly higher reduction (p < 0.005) bacterial numbers younger as well older biofilms treated...

Acne, a common skin disease in teenagers, is caused by Propionibacterium acnes (P. acnes). Isotretinoin (ITR) though reported to have immense antiacne potential, yet there are hardly any reports vouching its antimicrobial activity. The present study, therefore, was undertaken study the activity of ITR and evaluate effect encasement nanocarriers on minimum inhibitory concentration (MIC). were also evaluated for transport characteristics. MICs pure drug entrapped nanolipid carriers (ITR-NLCs)...

This study examined the therapeutic and prophylactic potential of bacteriophages in a mouse model Klebsiella pneumoniae lobar pneumonia. Phages were administered intraperitoneally. Liposome-entrapped phages (LP) effective treating infection, even when therapy was delayed by 3 days after induction In contrast, nonliposomal provided protection 24 hours infection. Administration 6 prior to intranasal bacterial challenge resulted complete protection, compared with LP, which 48 Increased...