A5070751489- Bryophyte Studies and Records
- Botany and Plant Ecology Studies
- Lichen and fungal ecology
- Peatlands and Wetlands Ecology
- Plant and animal studies
- Genetic diversity and population structure
- Microbial Community Ecology and Physiology
- Chromosomal and Genetic Variations
- Geology and Paleoclimatology Research
- Plant Pathogens and Resistance
- Protist diversity and phylogeny
- Plant Diversity and Evolution
- Yeasts and Rust Fungi Studies
- Ecology and Vegetation Dynamics Studies
- Insect and Arachnid Ecology and Behavior
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Taxonomy and Phylogenetics
- Marine and coastal plant biology
- Light effects on plants
- Evolution and Genetic Dynamics
- Climate change and permafrost
- Hibiscus Plant Research Studies
- Plant Parasitism and Resistance
University of Florida
2016-2025
University of Bonn
2023
University of Colorado System
2020
University of Colorado Boulder
2020
Cooperative Institute for Research in Environmental Sciences
2020
Northern Arizona University
2020
Washington University in St. Louis
2006-2017
Florida Museum of Natural History
2015
National Oceanic and Atmospheric Administration
2014
Duke University
2000-2008
The draft genome of the moss model, Physcomitrella patens, comprised approximately 2000 unordered scaffolds. In order to enable analyses structure and evolution we generated a chromosome-scale assembly using genetic linkage as well (end) sequencing long DNA fragments. We find that 57% comprises transposable elements (TEs), some which may be actively transposing during life cycle. Unlike in flowering plant genomes, gene- TE-rich regions show an overall even distribution along chromosomes....
Abstract Premise Bryophytes form a major component of terrestrial plant biomass, structuring ecological communities in all biomes. Our understanding the evolutionary history hornworts, liverworts, and mosses has been significantly reshaped by inferences from molecular data, which have highlighted extensive homoplasy various traits repeated bursts diversification. However, timing key events phylogeny, patterns, processes diversification across bryophytes remain unclear. Methods Using GoFlag...
We report the construction of a linkage map for moss Ceratodon purpureus (n = 13), based on cross between geographically distant populations, and provide first experimental confirmation maternal chloroplast inheritance in bryophytes. From mapping population 288 recombinant haploid gametophytes, genotyped at 121 polymorphic AFLP loci, three gene-based nuclear one marker, sex, we resolved 15 groups resulting length approximately 730 cM. estimate that covers more than three-quarters C. genome....
The widespread adoption of RAD-Seq data in phylogeography means genealogical relationships previously evaluated using relatively few genetic markers can now be addressed with thousands loci. One challenge, however, is that generates complete genotypes for only a small subset loci or individuals. Simulations indicate missing produce biased estimates key population parameters, although the influence such biases empirical studies not well understood. Here we compare microsatellite (8 loci) and...
Summary Mosses are critical components of boreal ecosystems where they typically account for a large proportion net primary productivity and harbour diverse bacterial communities that can be the major source biologically‐fixed nitrogen in these ecosystems. Despite their ecological importance, we have limited understanding how microbial vary across moss species extent to which local site conditions may influence composition communities. We used marker gene sequencing analyze associated with...
Moss sex chromosomes retain thousands of broadly expressed genes despite millions years suppressed recombination.
PREMISE New sequencing technologies facilitate the generation of large‐scale molecular data sets for constructing plant tree life. We describe a new probe set target enrichment to generate nuclear sequence build phylogenetic trees with any flagellate land plants, including hornworts, liverworts, mosses, lycophytes, ferns, and all gymnosperms. METHODS leveraged existing transcriptome genome design GoFlag 451 probes, 56,989 probes exons that are found in 248 single‐copy or low‐copy genes...
INTRODUCTION The moss Physcomitrella patens has been used as an experimental organism for more than 80 years. Within the last 15 years, its use a model to explore plant functions increased enormously. ability gene targeting and RNA interference methods study function, availability of many tools comparative functional genomics (including sequenced assembled genome, physical genetic maps, 250,000 expressed sequence tags [ESTs]), dominant haploid phase that allows direct forward analysis have...
INTRODUCTION This article includes a series of methods for culturing the moss Physcomitrella patens at all stages its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage gametophytes, cultures maintained medium 10°C very short day. Cryopreservation may also be used. Finally, sporophytes generated by self-fertilization sexual crossing.
The origin and maintenance of separate sexes (dioecy) is an enduring evolutionary puzzle. Although both hermaphroditism dioecy occur in many diverse clades, we know little about the long-term consequences changing sexual system. Here find evidence for at least 133 transitions between systems mosses, representing almost unparalleled lability evolution their systems. Furthermore, contrast to predictions, transition rate from was approximately twice as high reverse transition. Our results also...
Mosses in high-latitude ecosystems harbor diverse bacterial taxa, including N2-fixers which are key contributors to nitrogen dynamics these systems. Yet the relative importance of moss host species, and environmental factors, structuring microbial communities their N2-fixing potential remains unclear. We studied 26 boreal tundra species across 24 sites Alaska, USA, from 61 69° N. used cultivation-independent approaches characterize variation moss-associated as a function identity site...
Many bryophyte species have distributions that span multiple continents. The hypotheses historically advanced to explain such rely on either long-distance spore dispersal or slow rates of morphological evolution following ancient continental vicariance events. We use phylogenetic analyses DNA sequence variation at three chloroplast loci (atpB-rbcL spacer, rps4 gene, and trnL intron 3′ spacer) examine these two in the trans-Antarctic moss Pyrrhobryum mnioides. find: (1) reciprocal monophyly...
Abstract The moss Ceratodon purpureus has long been used as a model system in plant development and physiology. However, the molecular population genetics of species remains virtually unexplored. In this study, we genetic analyses DNA sequence data from three unlinked loci ( atpB‐rbcL spacer, adk , phy2 ) to examine biogeographical patterns global sample species. differed significantly mutation frequency spectra implied structure. Pairs haplotypes single populations were frequently more...
• The sequencing of the Physcomitrella patens genome, combined with high frequency gene targeting in this species, makes it ideal for reverse genetic studies. For forward studies, experimental crosses and analysis progeny are essential. Since P. is monoicous, producing both male female gametes on same gametophore, undergoing self-fertilization at a frequency, identification crossed sporophytes difficult. Usually spores from many mixed culture must be tested production recombinant progeny....
The moss Physcomitrella patens (Hedw.) Bruch & Schimp. is an important experimental model system for evolutionary-developmental studies. In order to shed light on the evolutionary history of and related species within Funariaceae, we analyzed natural genetic diversity Physcomitrium-Physcomitrella complex.Molecular analysis nuclear single copy gene BRK1 reveals that three Physcomitrium feature larger genome sizes than encode two expressed homeologs (polyploidization-derived paralogs),...
Introduction Half a century since the creation of International Association Bryologists, we carried out review to identify outstanding challenges and future perspectives in bryology. Specifically, have identified 50 fundamental questions that are critical advancing discipline.Methods We adapted deep-rooted methodology horizon scanning key research foci. An initial pool 258 was prepared by multidisciplinary international working group 32 bryologists. A series online surveys completed broader...
Abstract Background and Aims Resolving the phylogeny of hornworts is critical in understanding evolution key morphological characters that are unique to group, including pyrenoid. Extensive phylogenomic analyses have revealed unexpected complexities placement Leiosporoceros, previously identified sister taxon other hornworts. We explore role incomplete lineage sorting (ILS) ancient reticulation resolving interrelationships comprehending diversification evolutionary processes within Methods...
Many bryophyte species have distributions that span multiple continents. The hypotheses historically advanced to explain such rely on either long‐distance spore dispersal or slow rates of morphological evolution following ancient continental vicariance events. We use phylogenetic analyses DNA sequence variation at three chloroplast loci (atpB‐rbcL spacer, rps4 gene, and trnL intron 3′spacer) examine these two in the trans‐Antarctic moss Pyrrhobryum mnioides. find: (1) reciprocal monophyly...
A central problem in evolutionary biology is identifying factors that promote the evolution of reproductive isolation. Among mosses, biogeographic evidence indicates potential for migration great, suggesting biological other than geographic isolation may be critical speciation this group. The moss Physcomitrella patens (Funariaceae) has long been used as a model interspecies hybridization and recently emerged an important system comparative genomics. We report genealogical analyses six loci...
The haplolepideous mosses (Dicranidae) form the second largest group of and are morphologically ecologically highly diverse. This review summarizes current state addresses most urgent remaining problems in unravelling systematic relationships mosses. main results early molecular phylogenetic reconstructions based on few chloroplast markers compared with recent approaches from different genomes as well a new phylogeny novel combination non-coding plastid (rps4-trnF region atpB-rbcL spacer)....
In diploid organisms, selfing reduces the efficiency of selection in removing deleterious mutations from a population. This need not be case for all organisms. Some plants, example, undergo an extreme form known as intragametophytic selfing, which immediately exposes recessive parental genome to selective purging. Here, we ask how effectively are removed such plants. Specifically, study extent accumulate predominantly and outcrossing pair moss species, using genome-wide transcriptome data....
Abstract The bryophytes are a morphologically and ecologically diverse group of plants that have recently emerged as major model systems for variety biological processes. In particular, the genome sequence moss, P hyscomitrella patens , has significantly enhanced our understanding evolution developmental processes in land plants. However, to fully explore diversity within bryophytes, we need additional genomic resources. Here, describe analyses transcriptomes male female isolate C eratodon...
Abstract Each year new exotic species are transported across the world through global commerce, causing considerable economic and ecological damage. An important component of managing invasion pathways is to identify source populations. Some most widespread haplodiploid ambrosia beetles. The ability mate with siblings (inbreed) their transportable food (symbiotic fungus) have enabled them colonize become pests plant nurseries, lumber, forests. One fastest spreading beetles Xylosandrus...