A5065894125- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammasome and immune disorders
- Fatty Acid Research and Health
- Atherosclerosis and Cardiovascular Diseases
- COVID-19 Clinical Research Studies
- Sphingolipid Metabolism and Signaling
- Hepatitis C virus research
- Adipose Tissue and Metabolism
- Pediatric health and respiratory diseases
- Diabetes and associated disorders
- Systemic Lupus Erythematosus Research
- Reproductive System and Pregnancy
- Lipid metabolism and disorders
- Research on Leishmaniasis Studies
- SARS-CoV-2 and COVID-19 Research
- Exercise and Physiological Responses
- Synthesis and biological activity
- Cholesterol and Lipid Metabolism
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
Institute of Molecular and Translational Medicine
2019-2025
Palacký University Olomouc
2019-2025
University Hospital Olomouc
2023-2025
Central University of Venezuela
2015-2024
Regional Centre of Advanced Technologies and Materials
2022-2024
Cincinnati Children's Hospital Medical Center
2023
McGill University
2013-2020
McGill University Health Centre
2020
University of Luxembourg
2017
Arizona Oncology
2011-2015
We examined the effects of dietary n-3 polyunsaturated and saturated fatty acids on development atherogenic process in mice macrophage ability to secrete several effector molecules that may be involved process. The secretion inflammatory proteins such as tumor necrosis factor-alpha (TNF-alpha) interleukin-1 beta (IL-1 beta) production lipoprotein lipase (LPL), nitrogen oxide (NO2), prostaglandin E2 (PGE2) were evaluated peritoneal macrophages isolated from atherosclerosis-susceptible...
During pregnancy, women undergo several metabolic changes to guarantee an adequate supply of glucose the foetus. These modifications develop what is known as physiological insulin resistance. When this process altered, however, gestational diabetes mellitus (GDM) occurs. GDM a multifactorial disease, and genetic environmental factors play crucial role in its aetiopathogenesis. has been linked both macroscopic molecular alterations placental tissues that affect physiology. This review...
An inflammatory process may be involved in nitric oxide production skeletal muscle of type 2 diabetic patients. Nitric generation was assessed 14 non-complicated patients and 12 healthy subjects. In samples quadriceps femoris muscle, endothelial synthase (eNOS), inducible (iNOS), nitrite, nitrate nitrotyrosine were determined. The macrophage-specific antigen CD163, the T-cell membrane factor CD154 tumour necrosis factor-alpha (TNF-alpha) also assayed. six patients, ultrastructural analysis...
In the present study, we have investigated mechanisms underlying mouse resistance to endobronchial infection with Pseudomonas aeruginosa enmeshed in agar beads. This was done by monitoring macrophage activation-associated gene expression lung and alveolar cells harvested from resistant (BALB/c) susceptible (DBA/2, C57BL/6, A/J) strains of mice over course P. aeruginosa. Interleukin-1 alpha, interleukin-1 beta, inflammatory protein-1 JE, tumor necrosis factor alpha (TNF-alpha) mRNA levels...
SUMMARY In the present study, we evaluated NK cell cytotoxic activity in a group of HCV-infected individuals. Although number cells peripheral blood patients was comparable to non-infected individuals, spontaneous cytotoxicity four-fold lower (P < 0.001) than normal donors. This functional impairment not overcome by depletion adherent or B cells, and it partially restored short-term (18 h) stimulation with IL-2. However, long-term (72 this lymphokine induced activated killer (LAK)...
In chronic obstructive pulmonary disease (COPD) the presence of systemic inflammation has been associated with peripheral muscle abnormalities and weight loss. To study whether inflammatory factors are important in these processes, present compared skeletal levels nitrite, nitrate, nitrotyrosine, neuronal, endothelial inducible nitric oxide synthases (nNOS, eNOS, iNOS, respectively), markers (tumour necrosis factor (TNF)-α, CD154 CD163) 15 patients (forced expiratory volume one second...
Chronic and persistent lung infections cause the majority of morbidity mortality in patients with cystic fibrosis (CF). Galactosyl ceramide has been previously shown to be involved Pseudomonas internalization. Therefore, we assessed levels plasma CF compared them healthy volunteers using high-performance liquid chromatography followed by mass spectrometry. Our results demonstrate that display significantly lower several sphingolipid species, specifically C14:0, C20:1, C22:0, C22:1, C24:0...
The natural resistance associated macrophage protein 1 (Nramp1) gene determines the ability of murine macrophages to control infection with a group intracellular pathogens, including Salmonella typhimurium, Leishmania donovani, and Mycobacterium bovis bacillus Calmette-Guérin (BCG). expression resistant allele Nramp1 in is more efficient several activation-associated genes, class II MHC loci. In this study, we investigated molecular mechanisms involved IFN-gamma-induced three types...
Patients with cystic fibrosis (CF) and Cftr-knockout mice (CF mice) display an imbalance in fatty acids, high arachidonic acid (AA) low docosahexaenoic (DHA) concentrations. Our recent studies demonstrated defects another class of lipids, ceramides, patients CF mice. This study investigates the relationship between ceramide, AA, DHA, correction lipid imbalances after treatment fenretinide. Concentrations ceramide were assessed plasma from 58 adult 72 healthy control subjects. After 28 days...
In patients with systemic lupus erythematosus (SLE) metabolic alterations are often observed, which may be due to either the disease, genetic background or treatment. We studied serum levels of adipokines leptin, adiponectin, resistin, visfatin and ghrelin in SLE controls. Leptin were lower higher patients. No significant differences encountered for resistin. The values independent treatment, even after correction body mass index. Inverse correlations found among leptin visfatin. conclude...
A deficiency in cystic fibrosis transmembrane conductance regulator (CFTR) function CF leads to chronic lung disease. is associated with abnormalities fatty acids, ceramides, and cholesterol, their relationship pathology not completely understood. Therefore, we examined the impact of CFTR on lipid metabolism pro-inflammatory signaling airway epithelium using mass spectrometric, protein array. We observed a striking imbalance acid ceramide metabolism, oxidative stress under basal conditions...
The inflammatory response is thought to contribute secondary damage after spinal cord injury (SCI). Polyunsaturated fatty acids (PUFAs) play an important role in the onset and resolution of inflammation. Arachidonic acid (AA), ω-6 PUFA, contributes initiation responses, whereas docosahexaenoic (DHA), ω-3 has antiinflammatory effects. Therefore, decreasing AA increasing DHA levels SCI might be expected attenuate inflammation promote tissue protection functional recovery. We show here that...
Reactive oxygen species (ROS) are essential for sperm function. However, excessive ROS production can impair function and might be a factor contributing to male infertility. We investigated the levels of arachidonic acid (AA) docosahexaenoic (DHA) as well lipid peroxidation, represented by thiobarbituric reactive (TBARS), in blood seminal plasma 38 normozoospermic males from infertile couples (NSI-males), compared with that 17 fertile volunteers (FV-males). TBARS were higher NSI-males than...
Introduction Pulmonary exacerbations (PEs) cause significant morbidity and can severely impact disease progression in cystic fibrosis (CF) lung disease, especially patients who suffer from recurrent PEs. The assessments able to predict a future PE or are limited. We hypothesized that combining clinical, molecular patient reported data could identify at risk of PE. Methods prospectively followed cohort 53 adult CF for 24 months. Baseline values spirometry, clinical status using the Matouk...