The pro-oncogenic anterior gradient 2 (AGR2) is involved in tumor growth and drug resistance of breast cancer. Mechanisms that regulate expression AGR2 still need to be elucidated. In this study, levels miR-135b-5p were analyzed different cancer cell lines as well clinical tissues. vitro vivo functional effect also investigated. A luciferase reporter assay was applied confirm the interaction between mRNA. We identified a target miR-135b-5p. Expression up-regulated doxorubicin-resistant...

RUNX3 (runt-related transcription factor-3) is a known tumor suppressor gene which exhibits potent antitumor activity in several carcinomas. However, little about the role of human renal cell carcinoma (RCC). To investigate clinical relevance RCC patients, immunohistochemistry was performed to detect 75 tissues and paired non-cancerous by using tissue microarray (TMA). We also investigated migration, invasion angiogenesis. The expression decreased dramatically tissue. significantly...

Arsenic trioxide (As₂O₃), a traditional remedy in Chinese medicine, has been used acute promyelocytic leukemia (APL) research and clinical treatment. Previous studies have shown that As₂O₃ exerts its potent antitumor effects solid tumors by regulating cell proliferation survival. The aim of this study was to investigate whether inhibited gastric cancer migration angiogenesis FOXO3a expression. We found reduced viability dose-dependent manner also vitro. Western blotting immunofluorescence...

Previous studies have demonstrated that QX-314, an intracellular sodium channel blocker, can enter into nociceptors through capsaicin-activated TRPV1 or permeation of the membrane by chemical enhancers to produce a sensory-selective blockade. However, obvious side effects these combinations limit application QX-314. A new strategy for targeting delivery QX-314 needs further investigation. The aim this study is test whether acidic when dissolves in solution directly, acid-activated and block...

Currently, arsenic has been clinically investigated as a therapeutic agent for variety of solid malignancies, including breast cancer. However, the exact underlying molecular mechanisms through which trioxide (As2O3) induces cell growth arrest and apoptosis in tumors have not clearly understood. The aim our study was to gain an insight into effect As2O3 on human cancer MCF-7 line investigate inhibition, apoptosis, mechanism after treatment cells. Expression FOXO3a, nuclear-FOXO3a, caspase-3,...

MAP4K1 has been identified as a cancer immunotherapy target. Whether and how cell-intrinsic contributes to glioblastoma multiforme (GBM) progression remains unclear. We found that was highly expressed in the glioma cells of human GBM specimens. High levels mRNA were prevalent IDH-WT 1p/19q non-codeletion gliomas correlated with poor prognosis patients. silencing inhibited cell proliferation growth. Transcriptome analysis patient samples showed modulated cytokine‒cytokine receptor...

To investigate the role of RAD001 in reversing drug resistance SGC7901/DDP, we cultured SGC7901/DDP cells with different groups drugs (RAD001, cisplatin (DDP) alone, or combination and DDP); after that, detected sensitivity, cell apoptosis, levels P-gp, MRP1, survivin by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphe-nyltetrazolium bromide) assay, flow cytometry, immunohistochemical analysis, Western blot analysis. There was no significant difference between DDP 2.5-mg/L group negative...

The role of Runt-related transcription factor 3 ( RUNX3) gene in breast cancer remains not fully understood. We studied the correlation between RUNX3 promoter methylation and estrogen receptor (ER) expression status cancer. Three cell lines 113 formalin-fixed, paraffin-embedded tissue samples were analyzed for expression. Methylation-specific polymerase chain reaction was used to analyze on samples. Migration invasion ability evaluated MCF7 line (RUNX3 methylated) treated with inhibitor...

The incidence of thyroid disorders, which are common endocrine diseases, has rapidly increased in recent years. However, the etiology and pathogenesis these disorders remain unclear. Phosphatase tension homolog (PTEN) is a dual‑specific phosphatase that associated with multiple disorders; however, role PTEN remains unknown. In present study, human follicular epithelial cell line Nthy‑Ori 3‑1 was used to determine disorders. expression knocked down using PTEN‑specific short hairpin RNA....

Proper spindle formation and accurate chromosome segregation are essential for ensuring mitotic fidelity. Phosphatase tensin homolog (PTEN) is a multifunctional protein, which able to maintain the stability of genome chromosomes. The present study described an role PTEN in regulating prevent gross genomic instability via regulation arrest deficient 2 (MAD2). knockdown induced cell cycle abnormal segregation, manifested as anaphase bridges, lagging chromosomes premature chromatid separation....

A new isoflavone, 7,6’-dihydroxy-3’-methoxyisoflavone, has been isolated from the roots of Smilax glabra. The structure was determined by 2D-NMR techniques.

Abstract Arsenic trioxide (ATO) has been shown to have antitumor effect in different tumors, although the underlying mechanisms are not fully understood. Autophagy plays a critical role tumorigenesis and cancer therapy found be activated by ATO cells. However, of autophagy yet elucidated. In this study, we investigated antiangiogenic human umbilical vein endothelial cells (HUVECs) vitro its mechanism. Our data showed that suppresses angiogenesis induces HUVECs through upregulation forkhead...

Abstract Background CYP4 subfamily V member 2 ( CYP4V2 ) polymorphisms are related to venous thromboembolism. However, the influence of on susceptibility ischemic stroke (IS) remains undetermined. Methods We selected and genotyped five in 575 cases controls test whether variants were associated with risk for IS a Chinese Han population. Genotyping was performed using Agena MassARRAY platform. Logistic regression analysis used assess association between by calculating odds ratios (ORs) 95%...

Thorough excavation and artful utilization of various kinds concrete invisible learning resources contribute to the cultivation excellent postgraduates.In postgraduate education anesthesiology Xuzhou Medical College utilizes time,network,technique platform,research outcome,self-potentiality clinical patients resources,which produces an active effect has important instructional significance. Key words: Anesthesiology;  Postgraduate;  Resource