A5029444162- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Colorectal Cancer Treatments and Studies
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Renal Diseases and Glomerulopathies
- Inflammatory Biomarkers in Disease Prognosis
- Mesenchymal stem cell research
- Cancer, Lipids, and Metabolism
- Antifungal resistance and susceptibility
- CAR-T cell therapy research
- Wound Healing and Treatments
- Intraperitoneal and Appendiceal Malignancies
- Cytokine Signaling Pathways and Interactions
Brigham and Women's Hospital
2008-2024
Harvard University
2005-2024
University of Würzburg
2012-2022
Universitätsklinikum Würzburg
2011-2022
Clinical Trial Investigators
2022
Woman's Hospital
2020-2021
Molecular Oncology (United States)
2013-2021
Massachusetts General Hospital
2008
American Red Cross
2006
European Neuroendocrine Tumor Society
2006
Abstract Enhanced drug efflux mediated by ABCB1 P-glycoprotein and related ATP-binding cassette transporters is one of several mechanisms multidrug resistance thought to impair chemotherapeutic success in human cancers. In malignant melanoma, its potential contribution chemoresistance uncertain. Here, we show that ABCB5, which functions as a determinant membrane regulator cell fusion physiologic skin progenitor cells, expressed clinical melanoma tumors preferentially marks subset...
Interactions between chemokines and chemokine receptors have been proposed recently to be of importance in the development progression cancer. Human breast cancer cells express CXCL10 (IP-10) also its receptor CXCR3. In this study, we investigated role Ras activation regulation splice variant CXCR3-B two human cell lines MDA-MB-435 MCF-7. cotransfection assays, using a full-length promoter-luciferase construct, found that activated form Ras, Ha-Ras(12V), promoted transcriptional activation....
The drug efflux transporter ABCB5 identifies cancer stem-like cells (CSC) in diverse human malignancies, where its expression is associated with clinical disease progression and tumor recurrence. confers therapeutic resistance, but other functions tumorigenesis independent of have not been described that might help explain why it so broadly overexpressed cancer. Here we show melanoma-initiating cells, controls IL1β secretion, which serves to maintain slow cycling, chemoresistant through an...
There is evidence that inflammatory processes are involved in at least the early phase of complex regional pain syndrome (CRPS). We compared a panel pro- and antiinflammatory cytokines skin blister fluids serum from patients with CRPS upper-limb other origin (non-CRPS) stage (< 1 year) after 6 months treatment. Blister fluid was collected affected contralateral nonaffected side. used multiplex-10 bead array cytokine assay Luminex technology to measure protein concentrations interleukin-1...
Background Regulatory T cells (Treg) expressing the transcription factor forkhead-box protein P3 (Foxp3) have been identified to counteract anti-tumor immune responses during tumor progression. Besides, Foxp3 presentation by cancer itself may also allow them evade from effector T-cell responses, resulting in a survival benefit of tumor. For colorectal (CRC) clinical relevance has not evaluated detail. Therefore aim this study was its impact (CRC). Methods and Findings Gene analysis tissues...
Chronic inflammation as an important epigenetic and environmental factor for putative tumorigenesis tumor progression may be associated with specific activation of Toll-like receptors (TLR). Recently, carcinogenesis has been suggested to dependent on TLR7 signaling. In the present study, we determined role both TLR8 expression signaling in cell proliferation chemoresistance pancreatic cancer. Expression TLR7/TLR8 UICC stage I-IV cancer, chronic pancreatitis, normal tissue human (PANC1)...
The cytoprotective enzyme heme oxygenase-1 (HO-1) is often overexpressed in different types of cancers and promotes cancer progression. We have recently shown that the Ras-Raf-ERK pathway induces HO-1 to promote survival renal cells. Here, we examined possible mechanisms underlying HO-1-mediated cell survival. Considering growing evidence about significance apoptosis autophagy cancer, tried investigate how controls these events regulate Rapamycin (RAPA) sorafenib, two commonly used drugs for...
Abstract Background While rapid healing of diabetic foot ulcers (DFUs) is highly desirable to avoid infections, amputations and life-threatening complications, DFUs often respond poorly standard treatment. GMP-manufactured skin-derived ABCB5 + mesenchymal stem cells (MSCs) might provide a new adjunctive DFU treatment, based on their remarkable skin wound homing engraftment potential, ability adaptively inflammatory signals, healing-promoting efficacy in mouse models human chronic venous...
Signal transduction and activator of transcription 3 (STAT3) is a key factor implicated in the pathogenesis kidney fibrosis. Although Stat3 deletion tubular epithelial cells known to protect mice from fibrosis, vFoxd1 remains unclear. Using Foxd1-mediated knockout mice, CRISPR, inhibitors STAT3, we investigate its function. STAT3 phosphorylated acute injury, whereas it expanded interstitial fibrosis humans. protects folic-acid- aristolochic-acid-induced Mechanistically, upregulates...
The chemokine receptor CXCR3 may play a critical role in the growth and metastasis of tumor cells, including renal tumors. It has been shown that two splice variants with completely opposite functions; CXCR3-A promotes cell proliferation, whereas CXCR3-B inhibits growth. We recently demonstrated expression growth-promoting is up-regulated, growth-inhibitory markedly down-regulated human cancer tissues; overexpression cells can significantly inhibit proliferation. However, signal(s) through...
Cell-based strategies represent a new frontier in the treatment of immune-mediated disorders. However, paucity markers for isolation molecularly defined immunomodulatory cell populations poses barrier to this field. Here, we show that ATP-binding cassette member B5 (ABCB5) identifies dermal immunoregulatory cells (DIRCs) capable exerting therapeutic functions through engagement programmed death 1 (PD-1). Purified Abcb5+ DIRCs suppressed T proliferation, evaded immune rejection, homed...
The antidepressant fluoxetine has been under discussion because of its potential influence on cancer risk. It was found to inhibit the development carcinogen-induced preneoplastic lesions in colon tissue, but mechanisms action are not well understood. Therefore, we investigated anti-proliferative effects, and used HT29 tumor cells vitro, as C57BL/6 mice exposed intra-rectal treatment with carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) models. Fluoxetine increased percentage G0/G1...
The stress-inducible cytoprotective enzyme heme oxygenase-1 (HO-1) may play a critical role in the growth and metastasis of tumors. We demonstrated that overexpressed HO-1 promotes survival renal cancer cells by inhibiting cellular apoptosis; we also showed proto-oncogene H-Ras becomes activated these under stress following treatment with immunosuppressive agents. However, it is not known if there an association between Ras activation overexpression. Here, examined pathway could induce HO-1,...
Prostate specific antigen is not reliable in diagnosing prostate cancer (PCa), making the identification of novel, precise diagnostic biomarkers important. Since chemokines are associated with more aggressive disease and poor prognosis diverse malignancies, we aimed to investigate relevance PCa.Preoperative early postoperative serum samples were obtained from 39 consecutive PCa patients undergoing radical prostatectomy. Serum 15 healthy volunteers served as controls. Concentrations CXCL12,...
Toll like receptor (TLR) signaling has been suggested to play an important role in the inflammatory microenvironment of solid tumors and through this inflammation-mediated tumor growth. Here, we studied cells their process self-maintaining TLR expression independent cytokine milieu for autoregulative growth pancreatic cancer. We analyzed TLR2, -4, -9 primary human cancers impact on via induced activation several established cancers. TLR-stimulated cancer were specifically investigated...
Tumor models based on cancer cell lines cultured two-dimensionally (2D) plastic lack histological complexity and functionality compared to the native microenvironment. Xenogenic mouse tumor display higher but often do not predict human drug responses accurately due species-specific differences. We present here a three-dimensional (3D) in vitro colon model biological scaffold derived from decellularized porcine jejunum (small intestine submucosa+mucosa, SISmuc). Two different were used...
Platelet-derived growth factor (PDGF) and signaling via its receptors plays a crucial role in tumor cell proliferation thus may represent an attractive target besides VEGF/EGFR-based antibody therapies. In this study we analyzed the influence of PDGF colorectal cancer. was expressed intensively early even more late stage primary CRCs. Like VEGF, enhanced human colon cancer proliferation, increased oxidative glycolytic activity, activated HIF1α c-Myc vitro. PI3K/Akt/mTOR pathway while leaving...
Cardiovascular fibrosis is a major contributor to cardiovascular disease, the primary cause of death in patients with chronic kidney disease (CKD). We previously reported expression endogenous Klotho human arteries, and that CKD state deficiency, resulting vascular calcification, but myocardial poorly understood. This study aimed further clarify Klotho's functional roles cardiac underlying CKD.Human atrial appendage specimens were collected during surgery from individuals or without CKD....