A5003268078- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Animal Virus Infections Studies
- Bacterial Identification and Susceptibility Testing
- COVID-19 epidemiological studies
- Microbial Metabolism and Applications
- Immune responses and vaccinations
- Infectious Diseases and Tuberculosis
- Food Security and Health in Diverse Populations
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
Makerere University
2020-2025
Abstract Background Infection with the COVID-19-causing pathogen SARS-CoV-2 is associated disruption in human gut microbiome. The microbiome enables protection against diverse pathogens and exhibits dysbiosis during infectious autoimmune disease. Studies based United States China have found that severe COVID-19 cases altered composition when compared to mild cases. We present first study investigate of a population from Sub-Saharan Africa. Given impact geography cultural traditions on...
<ns3:p><ns3:bold>Background:</ns3:bold> In January 2020, a previously unknown coronavirus strain was identified as the cause of severe acute respiratory syndrome (SARS-CoV-2). The first viral whole-genome sequenced using high-throughput sequencing from sample collected in Wuhan, China. Whole-genome (WGS) is imperative investigating disease outbreak transmission dynamics and guiding decision-making public health.</ns3:p><ns3:p> </ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> We retrieved...
Abstract SARS-CoV-2 undergoes frequent mutations, affecting COVID-19 diagnostics, transmission and vaccine efficacy. Here, we describe the genetic diversity of 49 samples from Uganda, collected during waves 2020/2021. Overall, were similar to previously reported Uganda Democratic Republic Congo (DRC). The main lineages AY.46 A.23, which are considered be Delta variants. Further, a total 268 unique single nucleotide variants 1456 mutations found, with more than seventy percent in ORF1ab S...
<ns4:p><ns4:bold>Background: </ns4:bold>The emergence and spread of antiretroviral drug resistant HIV-1 variants is one the major factors associated with therapeutic failure in persons living HIV (PLWH) as it jeopardizes efforts to reduce progression AIDS. Whereas Sanger sequencing most appropriate conventional method for resistance testing, has limited capacity detect low-abundance variants. This study assessed suitability next generation (NGS) reveal mutations amongst patients experiencing...
Tackling antimicrobial resistance (AMR) is particularly challenging in low-resource settings such as Fort Portal Regional Referral Hospital (FPRRH) Western Uganda. Specific knowledge of local AMR epidemiology required to inform evidence-based improvement antibiotic stewardship measures the hospital. To address this, we combined existing susceptibility testing (AST) from FPRRH, with whole genome sequencing (WGS) 41 Staphylococcus aureus isolates (2017–2019). AST revealed 73 % (30 41) were...
Background: Infection with the COVID-19-causing pathogen SARS-CoV-2 is associated disruption in human gut microbiome. The microbiome enables protection against diverse pathogens and exhibits dysbiosis during infectious autoimmune disease. Studies based United States China have found that severe COVID-19 cases altered composition when compared to mild cases. We present first study investigate of a population from Sub-Saharan Africa. Given impact geography cultural traditions on composition,...
Abstract Background Mycobacterium tuberculosis presents several lineages each with distinct characteristics of evolutionary status, transmissibility, drug resistance, host interaction, latency, and vaccine efficacy. Whole genome sequencing (WGS) has emerged as a new diagnostic tool to reliably inform the occurrence phylogenetic examine their relationship patient demographic multidrug-resistance development. Methods 191 isolates obtained from 2017/2018 Tanzanian resistance survey were...
Abstract Tackling antimicrobial resistance (AMR) is particularly challenging in low-resource settings such as Fort Portal Regional Referral Hospital (FPRRH) Western Uganda. Specific knowledge of local AMR epidemiology required to inform evidence-based improvement antibiotic stewardship measures the hospital. To address this, we combined existing susceptibility testing (AST) from FPRRH, with whole genome sequencing (WGS) 41 Staphylococcus aureus isolates (2017-2019). AST revealed 73% (30/41)...