A5064683134- Polyamine Metabolism and Applications
- Helicobacter pylori-related gastroenterology studies
- Sulfur Compounds in Biology
- Eosinophilic Esophagitis
- Gastrointestinal disorders and treatments
- Mast cells and histamine
- Cannabis and Cannabinoid Research
- Ocular Infections and Treatments
- Neonatal Respiratory Health Research
- Probiotics and Fermented Foods
- Pediatric health and respiratory diseases
- Macrophage Migration Inhibitory Factor
- Medical Device Sterilization and Disinfection
- Genomics, phytochemicals, and oxidative stress
- Clinical Nutrition and Gastroenterology
- Amino Acid Enzymes and Metabolism
- Digestive system and related health
- Ocular Diseases and Behçet’s Syndrome
- Gut microbiota and health
- Ocular Oncology and Treatments
Vanderbilt University Medical Center
2019-2024
University of Tennessee Health Science Center
2023
Innate responses of myeloid cells defend against pathogenic bacteria via inducible effectors. Deoxyhypusine synthase (DHPS) catalyzes the transfer N-moiety spermidine to lysine-50 residue eukaryotic translation initiation factor 5A (EIF5A) form amino acid hypusine. Hypusinated EIF5A (EIF5AHyp) transports specific mRNAs ribosomes for translation. We show that DHPS is induced in macrophages by two gastrointestinal pathogens, Helicobacter pylori and Citrobacter rodentium, resulting enhanced...
Macrophages play a crucial role in the inflammatory response to human stomach pathogen Helicobacter pylori, which infects half of world's population and causes gastric cancer. Recent studies have highlighted importance macrophage immunometabolism their activation state function. We demonstrated that cysteine-producing enzyme cystathionine γ-lyase (CTH) is upregulated humans mice with H. pylori infection. Here, we show induction CTH macrophages by promoted persistent inflammation. Cth–/– had...
The reverse transsulfuration pathway is the major route for metabolism of sulfur-containing amino acids. role this metabolic in macrophage response and function unknown. We show that enzyme cystathionine γ-lyase (CTH) induced macrophages infected with pathogenic bacteria through signaling involving phosphatidylinositol 3-kinase (PI3K)/MTOR transcription factor SP1. This results synthesis cystathionine, which facilitates survival pathogens within myeloid cells. Our data demonstrate expression...
Hypusine is an amino acid synthesized by the enzyme deoxyhypusine synthase (DHPS). It critical for activity of eukaryotic translation initiation factor 5A (EIF5A). We reported that hypusination i) in macrophages supports innate response towards pathogenic bacteria and ii) epithelial cells maintains intestinal homeostasis. Herein, we investigated effect myeloid on outcome colitis colitis-associated cancer. found patients with Crohn's disease exhibit increased levels DHPS EIF5AHyp infiltrating...
To report the case of a 69-year-old male who was referred for previously unidentified pigmented iris lesion with surrounding atrophy masquerading as an melanoma.A sharply demarcated extending from trabecular meshwork to pupillary margin identified in left eye. There adjacent stromal atrophy. Testing consistent cyst-like lesion. The patient later described prior episode ipsilateral herpes zoster involving ophthalmic division cranial nerve five.Iris cysts present uncommon tumor, often going...