A5054328661- Polyamine Metabolism and Applications
- Helicobacter pylori-related gastroenterology studies
- Eosinophilic Esophagitis
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Food Allergy and Anaphylaxis Research
- Probiotics and Fermented Foods
- Plant Reproductive Biology
- Cancer Research and Treatments
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Amino Acid Enzymes and Metabolism
- Respiratory and Cough-Related Research
- Genomics, phytochemicals, and oxidative stress
- Nutrition, Genetics, and Disease
- Biopolymer Synthesis and Applications
- Inflammatory Bowel Disease
- Peanut Plant Research Studies
- Sulfur Compounds in Biology
- Diet and metabolism studies
- Microscopic Colitis
- Immune cells in cancer
- Transgenic Plants and Applications
- Respiratory viral infections research
- Bacteriophages and microbial interactions
Vanderbilt University Medical Center
2020-2024
Cleveland Clinic
2023-2024
Vanderbilt University
2022-2023
University of Massachusetts Amherst
2020-2022
Crohn's disease (CD) is a complex chronic inflammatory disorder with both gastrointestinal and extra-intestinal manifestations associated immune dysregulation. Analyzing 202,359 cells from 170 specimens across 83 patients, we identify distinct epithelial cell type in terminal ileum ascending colon (hereon as 'LND') high expression of LCN2, NOS2, DUOX2 genes related to antimicrobial response immunoregulation. LND cells, confirmed by in-situ RNA protein imaging, are rare non-IBD controls but...
Macrophages play a crucial role in the inflammatory response to human stomach pathogen Helicobacter pylori, which infects half of world's population and causes gastric cancer. Recent studies have highlighted importance macrophage immunometabolism their activation state function. We demonstrated that cysteine-producing enzyme cystathionine γ-lyase (CTH) is upregulated humans mice with H. pylori infection. Here, we show induction CTH macrophages by promoted persistent inflammation. Cth–/– had...
Helicobacter pylori is the primary cause of gastric cancer, and there a need to discover new molecular targets for therapeutic intervention in H. disease progression. We have previously shown that spermine oxidase (SMOX), enzyme catabolizes back-conversion polyamine spermidine, upregulated during infection associated with increased cancer risk humans. sought determine direct role SMOX carcinogenesis infection. In this study, we demonstrate transgenic FVB/N insulin-gastrin (INS-GAS) mice...
A signaling complex comprising members of the LORELEI (LRE)-LIKE GPI-anchored protein (LLG) and Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) families perceive RAPID ALKALINIZATION FACTOR (RALF) peptides regulate growth, reproduction, immunity, stress responses in Arabidopsis (Arabidopsis thaliana). Genes encoding these proteins are multigene most angiosperms could generate thousands variants. However, links between expansion gene functional diversification this critical as well...
Stomach cancer is a leading cause of death. Helicobacter pylori bacterial gastric pathogen that the primary risk factor for carcinogenesis, associated with its induction inflammation and DNA damage. Dicarbonyl electrophiles are generated from lipid peroxidation during inflammatory response form covalent adducts amine-containing macromolecules. 2-hydroxybenzylamine (2-HOBA) natural compound derived buckwheat seeds acts as potent scavenger reactive aldehydes. Our goal was to investigate effect...
Pathogenic enteric Escherichia coli present a significant burden to global health. Food-borne enteropathogenic E. (EPEC) and Shiga toxin-producing (STEC) utilize attaching effacing (A/E) lesions actin-dense pedestal formation colonize the gastrointestinal tract. Talin-1 is large structural protein that links actin cytoskeleton extracellular matrix though direct influence on integrins. Here we show mice lacking talin-1 in intestinal epithelial cells (Tln1Δepi) have heightened susceptibility...
Abstract Colonization by Helicobacter pylori is associated with gastric diseases, ranging from superficial gastritis to more severe pathologies, including intestinal metaplasia and adenocarcinoma. The interplay of the host response pathogen affect outcome disease. One major component mucosal H. activation a strong but inefficient immune that fails control infection frequently causes tissue damage. We have shown polyamines can regulate pylori–induced inflammation. Chemical inhibition...
Abstract Cystathionine γ-lyase (CTH) is a critical enzyme in the reverse transsulfuration pathway, major route for metabolism of sulfur-containing amino acids, notably converting cystathionine to cysteine. We reported that CTH supports gastritis induced by pathogen Helicobacter pylori . Herein our aim was investigate role colonic inflammation. First, we found colon mucosa mice with dextran sulfate sodium-induced colitis. Expression completely absent Cth –/– mice. observed clinical and...
Enteropathogenic Escherichia coli (EPEC) is a bacterium that causes attaching/effacing (A/E) lesions and serious diarrheal disease, major health issue in developing countries. EPEC pathogenicity results from the effect of virulence factors dysregulation host responses. Polyamines, including spermidine, play role intestinal homeostasis. Spermidine substrate for deoxyhypusine synthase (DHPS), which catalyzes conjugation amino acid hypusine to eukaryotic translation initiation factor 5A...
Abstract A signaling complex comprising members of the LORELEI (LRE)-LIKE GPI-anchored protein (LLG) and Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE ( Cr RLK1L) families perceive RAPID ALKALINIZATION FACTOR (RALF) peptides regulate growth, reproduction, immunity, stress responses in Arabidopsis. Genes encoding these proteins are multi-gene most angiosperms could generate thousands variants. However, link(s) between expansion gene functional diversification this critical as well...
Background and Purpose: Colorectal cancer (CRC) is a major health problem worldwide. Dicarbonyl electrophiles, such as iso-levuglandins (isoLGs), are generated from lipid peroxidation form covalent adducts with amine-containing macromolecules. We have shown high levels of isoLGs in colonic epithelial cells patients CRC. thus investigated the role these reactive aldehydes on colon development. Experimental Approach: effect oral treatment 2-hydroxybenzylamine (2-HOBA), natural compound derived...
Abstract INTRODUCTION Aconitate decarboxylase 1 (ACOD1; also known as IRG1) is highly expressed in activated macrophages and converts cis-aconitate to itaconate, which contributes the antimicrobial activity of macrophages. The role ACOD1 intestinal inflammation remains unknown. Therefore, we investigated during colitis induced by Citrobacter rodentium (C. rodentium), a murine bacterial pathogen used model pathogenesis enteropathogenic Escherichia coli, dextran sulfate sodium (DSS) epithelial...