A5073639712- Birth, Development, and Health
- Eicosanoids and Hypertension Pharmacology
- Vector-borne infectious diseases
- Gestational Diabetes Research and Management
- Peroxisome Proliferator-Activated Receptors
- Viral Infections and Vectors
- Alcohol Consumption and Health Effects
- Pregnancy and preeclampsia studies
- Adipose Tissue and Metabolism
- Diabetes Management and Research
- Pancreatic function and diabetes
- Cystic Fibrosis Research Advances
- Metabolism, Diabetes, and Cancer
- Forensic Toxicology and Drug Analysis
- Hormonal Regulation and Hypertension
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cannabis and Cannabinoid Research
- Vector-Borne Animal Diseases
- Endoplasmic Reticulum Stress and Disease
- Machine Learning in Bioinformatics
- Toxin Mechanisms and Immunotoxins
- Cardiovascular Disease and Adiposity
- Diet and metabolism studies
- Thermal Radiation and Cooling Technologies
- Cancer Risks and Factors
Shanghai Veterinary Research Institute
2020-2025
Chinese Academy of Agricultural Sciences
2016-2025
Aalto University
2023
University of Iowa Stead Family Children’s Hospital
2022
University of Iowa
2004-2018
Institute of Animal Sciences
2016
Boston Children's Hospital
2014
Pediatrics and Genetics
2012
Instituto de Nutricion e Higiene de los Alimentos
2000
Abstract Inspired by biological systems, trainable responsive materials have received burgeoning research interests for future adaptive and intelligent material systems. However, the to date typically cannot perform active work, training allows only one direction of functionality change. Here, we demonstrate thermally hydrogel systems consisting two thermoresponsive polymers, where volumetric response system upon phase transitions enhances or decreases through a process above certain...
Diabetes is a common comorbidity in cystic fibrosis (CF) that worsens prognosis. The lack of an animal model for CF-related diabetes (CFRD) has made it difficult to dissect how the onset pancreatic pathology influences emergence CFRD. We evaluated structure and function neonatal CF endocrine pancreas using new CFTR-knockout ferret model. Although kits are born with only mild exocrine disease, progressive loss during first months life was associated inflammation, spontaneous hyperglycemia,...
Fat-tailed sheep have commercial value because consumers prefer high-protein and low-fat food producers care about feed conversion rate. However, fat-tailed still some scientific significance, as the fat tail is commonly regarded a characteristic of environmental adaptability. Finding candidate genes associated with formation essential for breeding conservation. To identify these genes, we applied FST hapFLK approaches in fat- thin-tailed available 50K SNP genotype data. These two methods...
Epoxyeicosatrienoic acids (EETs), lipid mediators synthesized from arachidonic acid by cytochrome P-450 epoxygenases, are converted soluble epoxide hydrolase (SEH) to the corresponding dihydroxyeicosatrienoic (DHETs). Originally considered as inactive degradation products of EETs, DHETs have biological activity in some systems. Here we examined capacity EETs and activate peroxisome proliferator-activated receptor-α (PPARα). We find that among EET DHET regioisomers, 14,15-DHET is most potent...
B. microti is a tick-transmitted zoonotic erythrocytic intracellular parasite. Ferroptosis an iron-dependent form of programmed cell death that affects pathogen replication in the host. Currently, there limited research concerning effect tick ferroptosis on Babesia infection and underlying mechanism action. The present study used -mouse- Haemaphysalis longicornis model which nymphs fed blood -infected mice. midgut divalent iron ( p<0.01 ) reactive oxygen species (ROS) p<0.05...
We investigated the effects of soluble epoxide hydrolase (sEH) inhibition on epoxyeicosatrienoic acid (EET) metabolism in intact human blood vessels, including saphenous vein (HSV), coronary artery (HCA), and aorta (HA). When HSV segments were perfused with 2 micromol/l 14,15-[3H]EET for 4 h, >60% radioactivity perfusion medium was converted to 14,15-dihydroxyeicosatrienoic (DHET). Similar results obtained endothelium-denuded vessels. 14,15-DHET released from both luminal adventitial...
The ability of dietary enrichment with monounsaturated fatty acid (MUFA), n-3 or n-6 polyunsaturated acids (PUFAs) to reverse glucose intolerance and vascular dysfunction resulting from excessive saturated is not resolved. We hypothesized that partial replacement fats PUFA-enriched menhaden oil (MO) would provide greater improvement in tolerance function compared enriched safflower (SO) MUFA-enriched olive (OO).We fed mice a high fat diet (HF) (60% kcal lard) for 12 weeks before substituting...
Abstract Background Apoptosis is fundamental in maintaining cell balance multicellular organisms, and caspases play a crucial role apoptosis pathways. It reported that plays an important tick salivary gland degeneration. Several different have been found ticks, but the interactions between them are currently unknown. Here, we report three new caspases, isolated from glands of Rhipicephalus haemaphysaloides . Methods The full-length cDNA RhCaspases 7, 8 9 genes were obtained by transcriptome,...
Soluble epoxide hydrolase (sEH) plays a major role in regulating vascular epoxyeicosatrienoic acid metabolism and function, substituted urea derivatives that inhibit sEH activity reduce blood pressure hypertensive rats. We found containing dodecanoic group, besides effectively inhibiting sEH, increased peroxisome proliferator-activated receptor (PPAR) α activity. In PPARα transfected COS-7 cells, treatment with 10 μM <i>N</i>-cyclohexyl-<i>N</i>′-dodecanoic (CUDA) or...
ODM (offspring of diabetic mothers) have an increased risk developing metabolic and cardiovascular dysfunction; however, few studies focused on the susceptibility to disease in offspring mothers diabetes during pregnancy. We developed animal model late gestation pregnancy characterized vascular function offspring. Diabetes was induced by streptozotocin (50 mg/kg body weight, intraperitoneally) pregnant rats gestational day 13 partially controlled twice-daily injections insulin. At 2 months...
Cardiac septal overgrowth complicates 10–40% of births from diabetic mothers, but perplexingly hyperglycemia markers during pregnancy are not reliably predictive. We thus tested whether fetal exposure to is sufficient induce cardiac even in the absence systemic maternal diabetes. To isolate effects hyperglycemia, we infused glucose into blood supply left right uterine horn nondiabetic pregnant rats starting on gestational day 19. After 24 h infusion, right-sided fetuses and dams remained...
20-Hydroxyeicosatetraenoic acid (20-HETE), an arachidonic (AA) metabolite synthesized by cytochrome P-450 omega-oxidases, is reported to produce vasoconstriction in the cerebral circulation. However, we find that like 14,15-epoxyeicosatrienoic (14,15-EET), 20-HETE produces dilation of mouse basilar artery preconstricted with U-46619 vitro. Indomethacin inhibited vasodilation produced but not 14,15-EET, suggesting a cyclooxygenase (COX)-dependent mechanism. Metabolic studies indicated several...
Peroxisome proliferator-activated receptor γ (PPARγ) is expressed at low levels in skeletal muscle, where it protects against adiposity and insulin resistance via unclear mechanisms. To test the hypothesis that PPARγ directly modulates muscle metabolism, we created two models isolate direct actions on myocytes. was overexpressed murine myotubes by adenotransfection mouse plasmid electroporation. In cultured myotubes, action increased fatty acid uptake incorporation into myocellular lipids,...
Offspring exposed in utero to maternal diabetes exhibit long-lasting insulin resistance, though the initiating mechanisms have received minimal experimental attention. Herein, we show that rat fetuses develop resistance after only 2-day continuous exposure isolated hyperglycemia starting on gestational day 18. Hyperglycemia-induced reductions insulin-induced AKT phosphorylation localized primarily fetal skeletal muscle. The muscle of hyperglycemia-exposed also exhibited impaired vivo glucose...
The growing fetus depends upon transfer of glucose from maternal blood to fetal tissues. Insulin and glucocorticoid impact metabolism, but the effects these hormones on assimilation in vivo are understudied. We thus used positron emission tomography imaging determine disposition [(18)F]fluorodeoxyglucose (FDG) rats gestational d 20, quantifying kinetic competition tissues for glucose. Three fasting states were studied: after 2-d dexamethasone (DEX), during euglycemic hyperinsulinemic clamp...
Abstract Bright white color is often achieved in nature by the combination of polydisperse scattering structures and high refractive index contrast between scatterer surrounding medium. Similarly, synthetic systems have commonly utilized inorganic materials as centers to achieve color, which, however, lacks ability switch optical properties. While hydrogels capable light are applications such smart windows, their reflection properties remained limited due low polymer water. As a result,...
Background Complex but common maternal diseases such as diabetes and obesity contribute to adverse fetal outcomes. Understanding of the mechanisms involved is hampered by difficulty in isolating individual elements complex states vivo. We approached this problem context sought an approach expose developing fetus vivo isolated hyperglycemia pregnant rat. Methodology Principal Findings hypothesized that glucose infused into arterial supply one uterine horn would more highly fetuses ipsilateral...
Ferrets are an important emerging model of cystic fibrosis related diabetes. However, there is little documented experience in the use advanced techniques to quantify aspects diabetes pathophysiology ferret. Glycemic clamps gold standard technique assess both insulin sensitivity and secretion humans animal models We therefore sought develop for glycemic ferrets. To sensitivity, we performed euglycemic hyperinsulinemic 5–6 week old ferrets anesthetized conscious states. secretion,...
Abstract Background The salivary glands of female ticks degenerate rapidly by apoptosis and autophagy after feeding. Bcl-2 family proteins play an important role in the pathways, but functions these are unclear. We studied Bax homologs from Rhipicephalus haemaphysaloides determined their degeneration glands. Methods Two molecules containing conserved BH (Bcl-2 homology) domains were identified named RhBcl-2 RhBax . After protein purification mouse immunization, specific polyclonal antibodies...