A5100331874- Pharmacogenetics and Drug Metabolism
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Computational Drug Discovery Methods
- Retinal Diseases and Treatments
- Phytochemicals and Antioxidant Activities
- Folate and B Vitamins Research
- Innovation in Digital Healthcare Systems
- Chromatography in Natural Products
- Clostridium difficile and Clostridium perfringens research
- Curcumin's Biomedical Applications
- Technology and Data Analysis
- Pharmacological Effects and Toxicity Studies
- Phytochemistry and Biological Activities
- Drug Transport and Resistance Mechanisms
- Cardiovascular Syncope and Autonomic Disorders
- Natural product bioactivities and synthesis
- Diet and metabolism studies
- Natural Antidiabetic Agents Studies
- Glaucoma and retinal disorders
- Coffee research and impacts
- Muscle metabolism and nutrition
- Gastrointestinal motility and disorders
- Biochemical effects in animals
- Liver physiology and pathology
Chinese University of Hong Kong
2025
Hanyang University
2017-2024
IIT@MIT
2015
Molecular networking (MN) has become a popular data analysis method for untargeted mass spectrometry (MS)/MS-based metabolomics. Recently, MN been suggested as powerful tool drug metabolite identification, but its effectiveness metabolism studies not yet benchmarked against existing strategies. In this study, we compared the performance of MN, defect filtering, Agilent MassHunter Metabolite ID, and Mass Profiler Professional workflows to annotate metabolites sildenafil generated in an vitro...
To investigate the associations of lung function with glaucoma and related traits, explore interactions between genetic risk function, assess causal relationships using Mendelian randomization (MR). This cross-sectional study involved 85,369 participants measurements at baseline from UK Biobank. Associations parameters traits were tested by multivariable logistic linear regression. Two-sample MR analyses conducted summary statistics large datasets. Forced vital capacity (FVC), forced...
In this study, we investigated the effects of treatment with Gingko biloba leaf extract (GLE) on intestinal transporter expression and gut microbiota composition in mice correlation between mice. When GLE was orally administered to mice, BCRP significantly suppressed. Pharmacokinetic studies showed that maximum plasma concentration area under curve values sulfasalazine were increased more than twice by compared those control group. decreased populations Proteobacteria Deferribacteres at...
Curcumin is a polyphenol compound derived from the rhizomes of Curcuma longa that exhibits antioxidant, anti-inflammatory, anticancer, and antimicrobial properties. However, its low solubility in aqueous solutions, absorption following oral administration, rapid degradation limit use as functional food material. In this study, hydroxypropyl methylcellulose-based solid dispersion curcumin (DW-CUR 20) was prepared bioavailability evaluated. addition, therapeutic efficacy hepatoprotective agent...
The NBOMe family is a group of new psychoactive substances (NPSs). In this study, the fragmentation patterns derivatives were analyzed using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF/MS). MS/MS spectral data was used to establish molecular networking map for derivatives. nine interpreted on basis their product ion data. generally showed similar patterns; among them, halogen-substituted methoxybenzyl ethanamine type characteristic radical cation. Molecular...
In this study, the pharmacokinetic profiles of bioactive components in leaf extract medicinal herb, Cudrania tricuspidate, were investigated using an MS/MS-based molecular networking system. To identify major active C. tricuspidate (CLE), HPLC-DAD analysis was conducted with a standard mixture six flavonoids (rutin, isoquercitrin, nicotiflorin, kaempferol 3-O-glucoside, quercetin, and kaempferol). The unknown peaks determined via mass dataset obtained by liquid chromatography quadrupole...
Caffeine is commonly taken via the daily dietary consumption of caffeine-containing foods. The absorbed caffeine metabolized to yield various metabolites by drug-metabolizing enzymes, and measuring levels each metabolite can provide useful information for evaluating phenotypes those enzymes. In this study, urinary concentrations its 13 were determined, drug metabolic enzymes investigated based on ratios. Human urine samples pretreated using solid phase extraction, analyzed liquid...
Abstract This study assessed the inhibitory effects of Garcinia cambogia extract on cytochrome P450 enzymes in vitro. G. was incubated with isozyme-specific substrates human liver microsomes and recombinant CYP2B6 isozyme, formation marker metabolites measured to investigate potential enzyme activities. The results showed that has significant activity a concentration-dependent manner. Furthermore, inhibition potentiated following preincubation NADPH, indicating is time-dependent inhibitor...
Traditionally cultured monolayers of primary human hepatocytes (PHHs) deteriorate within days and thereby become unsuitable for drug-related studies. PHH spheroids (3D PHHs) maintain liver functions weeks, but are considerably more demanding. Recently, a chemical-based approach (5C succeeded in long-term culture hepatocyte monolayers, it remains unclear whether the preserved. To clarify this, we compared 5C 3D PHHs terms gene expression analysis, proteomic functionality (basal induced...
Purpose: An early detailed understanding of organ-specific drug metabolism is crucial in discovery and development. Therefore, a robust quantitative high-throughput preclinical tool for the characterization metabolites, including reactive species, important. The aim this project was to assess fate human liver gut microphysiological systems (MPS) context. A UHPLC-HRMS method identification metabolites established using high mass accuracy, isotope pattern matching, MS/MS-based scoring...
Osteoarthritis is one of the leading conditions that promote consumption these dietary supplements. Chondroitin sulfate, glucosamine, and methylsulfonylmethane are among prominent alternative treatments for osteoarthritis. In this study, supplements were incubated with cytochrome P450 isozyme-specific substrates in human liver microsomes, formation marker metabolites was measured to investigate their inhibitory potential on enzyme activities. The results revealed no significant effects seven...