A5045671407- Adipose Tissue and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Obesity and Health Practices
- Neuroscience and Neuropharmacology Research
- Skin Protection and Aging
- Redox biology and oxidative stress
- Spectroscopy and Quantum Chemical Studies
- Neural dynamics and brain function
- Genetics, Aging, and Longevity in Model Organisms
- Obesity, Physical Activity, Diet
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Immunology Research
- Optical Imaging and Spectroscopy Techniques
- Glioma Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Virus-based gene therapy research
- Circadian rhythm and melatonin
- Biochemical effects in animals
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Advancements in Transdermal Drug Delivery
Nippon Medical School
2009-2024
Freie Universität Berlin
2015-2023
Morpho (United States)
2021
Michigan State University
2018-2019
Institute for Advanced Medical Research
2019
University of Alabama
2018
University of Virginia
1994-2014
Keio University
2009-2011
National Defense Academy of Japan
2011
National Defense Medical College
2011
Physical activity, inactivity, and obesity were assessed by questionnaire among a multiethnic sample of 552 girls in grades 5 through 12. Hispanics Asians reported lower activity levels than other racial groups. Only 36% the entire less one fifth either or met year 2000 goal for strenuous physical activity. was inversely associated with age age-adjusted body mass index. Obesity unrelated to inactivity.
Aldehyde accumulation is regarded as a pathognomonic feature of oxidative stress-associated cardiovascular disease.We investigated how the heart compensates for accelerated aldehydes.Aldehyde dehydrogenase 2 (ALDH2) has major role in aldehyde detoxification mitochondria, source aldehydes. Transgenic (Tg) mice carrying an Aldh2 gene with single nucleotide polymorphism (Aldh2*2) were developed. This dominant-negative effect and Tg exhibited impaired ALDH activity against broad range Despite...
Rationale: Caloric restriction (CR) confers cardioprotection against ischemia/reperfusion injury. However, the exact mechanism(s) underlying CR-induced remain(s) unknown. Recent evidence indicates that Sirtuins, NAD + -dependent deacetylases, regulate various favorable aspects of CR response. Thus, we hypothesized deacetylation specific mitochondrial proteins during preserves function and attenuates production reactive oxygen species ischemia/reperfusion. Objective: The objectives present...
Post-transcriptional taurine modification at the first anticodon ("wobble") nucleotide is deficient in A3243G-mutant mitochondrial (mt) tRNA(Leu(UUR)) of patients with myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS). Wobble modifications tRNAs have recently been identified to be important accurate efficient deciphering codons. We herein examined whether can alleviate dysfunction patient-derived pathogenic cells prevent clinical symptoms MELAS patients.The addition...
Micelacking both Kv3.1 and Kv3.3 K + channel alleles display severe motor deficits such as tremor, myoclonus, ataxic gait. one to three at the loci exhibit in an allele dose-dependent manner a modest degree of ataxia. Cerebellar granule cells coexpress channels are therefore candidate neurons that might be involved these behavioral deficits. Hence, we investigated synaptic mechanisms transmission parallel fiber-Purkinje cell system. Action potentials fibers were broader mice lacking single...
Abstract Nanoparticles hold a great promise in biomedical science. However, due to their unique physical and chemical properties they can lead overproduction of intracellular reactive oxygen species (ROS). As an important mechanism nanotoxicity, there is need for sensitive high‐throughput adaptable single‐cell ROS detection methods. Here, fluorescence lifetime imaging microscopy (FLIM) employed (FLIM‐ROX) providing increased sensitivity enabling analysis fixed live cells. FLIM‐ROX owes its...
Variation in the peroxisome proliferator-activated receptor γ (PPARγ) gene may play a role development of type 2 diabetes mellitus. Therefore we investigated association between P12A and c1431t polymorphisms PPARγ diabetes. The incidence polymorphism was determined by PCR-RFLP single-strand conformation analysis 219 patients with, 429 without frequency A allele 0.16 t polymorphism, 0.13 with diabetes, 0.12 respectively subjects 3.2% 1.4% were A12A. Since are not linked 9 possible genotypes...
The transcriptional coactivator PGC-1α has been implicated in the regulation of multiple metabolic processes. However, previously reported phenotypes mice deficient have inconsistent. exists as isoforms, including variants transcribed from an alternative first exon. We show here that are main entity increases during exercise. These variants, unlike canonical isoform PGC-1α, robustly upregulated human skeletal muscle after Furthermore, extent this upregulation correlates with oxygen...
The question whether nanoparticles can cross the skin barrier is highly debated. Even in intact rare events of deeper penetration have been reported, but technical limitations and possible artifacts require careful interpretation. In this study, horizontal scanning by 2‐photon microscopy (2 PM) full‐thickness human samples placed a lateral position yielded informative images for studies fluorescently tagged nanoparticles. Scanning large fields view allowed detailed information on...
We report here on the application of laser-based single molecule total internal reflection fluorescence microscopy (TIRFM) to study penetration molecules through skin. Penetration topically applied drug is often observed be limited by size respective drug. However, molecular mechanisms which govern outermost layer skin are still largely unknown. As a model compound we have chosen larger amphiphilic (fluorescent dye ATTO-Oxa12) with weight >700 Da that was excised human ATTO-Oxa12...
Despite continued increases in human life expectancy, the factors determining rate of biological aging remain unknown. Without understanding molecular mechanisms underlying aging, efforts to prevent are unlikely succeed. The tumor suppression theory introduced here proposes somatic mutation as proximal cause but postulates that oncogenic transformation and clonal expansion, not functional impairment, relevant consequences mutation. Obesity caloric restriction accelerate decelerate due their...
Aim: To summarize baseline characteristics, health conditions, resource utilization and cost for the US population 90‐day period preceding enrolment, stratified by body mass index (BMI) presence of abdominal obesity (AO). Methods: PROCEED (Prospective Obesity Cohort Economic Evaluation Determinants) is a multinational, prospective cohort control (BMI 20–24.0 kg/m 2 ), overweight 25–29.9 ) obese ≥ 30 subjects with AO without [non‐abdominal (NAO)], defined waist circumference (WC) >102 88...
Abstract The rapid development of microscopic techniques over the past decades enables establishment single molecule fluorescence imaging as a powerful tool in biological and biomedical sciences. Single allows to study chemical, physicochemical, properties target molecules or particles by tracking their molecular position environment determining dynamic behavior. However, precise determination particle distribution diffusivities is often challenging due high molecule/particle densities, fast...