A5063965497- TGF-β signaling in diseases
- Nerve injury and regeneration
- Corporate Governance and Management
- RNA Interference and Gene Delivery
- Bone Metabolism and Diseases
- RNA modifications and cancer
- Virus-based gene therapy research
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Neurogenesis and neuroplasticity mechanisms
- Bacterial Infections and Vaccines
- Extracellular vesicles in disease
- Reproductive Biology and Fertility
- Digital Innovation in Industries
- S100 Proteins and Annexins
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Ion channel regulation and function
- Pneumonia and Respiratory Infections
- Cardiac electrophysiology and arrhythmias
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
Universitätsmedizin Göttingen
2012-2024
University of Göttingen
2003-2021
German Center for Neurodegenerative Diseases
2017
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2006
Glasgow Life
2005
Eppendorf (Germany)
2000
Martini-Klinik
2000
University of Bern
1999
University of Münster
1993
Reproduktionsmedizin München
1993
Axonal degeneration is an initial key step in traumatic and neurodegenerative CNS disorders. We established a unique vivo epifluorescence imaging paradigm to characterize very early events axonal the rat optic nerve. Single retinal ganglion cell axons were visualized by AAV-mediated expression of dsRed this allowed quantification postlesional acute (AAD). EM analysis revealed severe structural alterations cytoskeleton, cytoplasmatic vacuolization, appearance autophagosomes within first hours...
The Rho/ROCK/LIMK pathway is central for the mediation of repulsive environmental signals in nervous system. Several studies using pharmacological Rho-associated protein kinase (ROCK) inhibitors have shown positive effects on neurite regeneration and suggest additional pro-survival neurons. However, as none these drugs completely target specific, it remains unclear how are mediated whether ROCK really most relevant pathway. To answer questions, we generated adeno-associated viral vectors to...
Abstract Many neuropathological and experimental studies suggest that the degeneration of dopaminergic terminals axons precedes demise neurons in substantia nigra, which finally results clinical symptoms Parkinson disease (PD). The mechanisms underlying this early axonal are, however, still poorly understood. Here, we examined effects overexpression human wildtype alpha-synuclein ( α Syn-WT), a protein associated with PD, its mutant variants Syn-A30P -A53T on neurite morphology functional...
Compared with beta-lactam antibiotics, rifampin releases smaller quantities of proinflammatory cell wall products from Streptococcus pneumoniae in vitro. Mice infected intracerebrally S. were treated subcutaneously 2-mg doses or ceftriaxone (n=43 each) every 12 h for 3 days and then observed another days. Rifampin reduced overall mortality 49% to 26% (P=.04). Kaplan-Meyer analysis revealed a substantial reduction during the first 24 mice receiving (difference survival time: P=.007). Eight...
OBJECTIVE: Activin is a growth and differentiation factor of many cell types, has recently been implicated in inflammatory processes. Clinical data linking activin its binding protein, follistatin (FS), are lacking. We measured serum levels FS patients diagnosed with septicemia. PATIENTS AND MEASUREMENTS: Eight male seven female different ages, various forms septicemia clinical outcome were investigated compared age- sex-matched healthy controls. Serum concentrations FS, activin, C-reactive...
Abstract Axonal degeneration is a key initiating event in many neurological diseases. Focal lesions to axons result rapid disintegration of the perilesional axon by acute axonal (AAD) within several hours. However, underlying molecular mechanisms AAD are only incompletely understood. Here, we studied vivo through live-imaging rat optic nerve and vitro primary cortical neurons microfluidic chambers. We found that calpain activated early during inhibition completely inhibits fragmentation on...
Abstract Axonal degeneration is a key and early pathological feature in traumatic neurodegenerative disorders of the CNS. Following focal lesion to axons, extended axonal disintegration by acute (AAD) occurs within several hours. During AAD, accumulation autophagic proteins including Unc-51 like autophagy activating kinase 1 (ULK1) has been demonstrated, but its role incompletely understood. Here, we study effect ULK1 inhibition different models lesion-induced vitro vivo. Overexpression...
Article abstract Lipoteichoic and teichoic acids are components of the cell wall Streptococcus pneumoniae. A recently developed enzyme immunoassay was used in patients with pneumococcal meningitis to investigate lipoteichoic acid concentrations CSF at first lumbar puncture relation clinical outcome determined by Glasgow Outcome Scale. were significantly associated neurologic sequelae mortality S. pneumoniae meningitis.
The Rho/ROCK pathway is a promising therapeutic target in neurodegenerative and neurotraumatic diseases. Pharmacological inhibition of various members has been shown to promote neuronal regeneration survival. However, because pharmacological inhibitors are inherently limited their specificity, shRNA-mediated approaches can add more information on the function each single kinase involved. Thus, we generated adeno-associated viral vectors (AAV) specifically downregulate Ras homologous member A...
Sporadic Creutzfeldt-Jakob disease (sCJD) is the most prevalent form of human prion and it characterized by presence neuronal loss, spongiform degeneration, chronic inflammation accumulation misfolded pathogenic protein (PrPSc). The molecular mechanisms underlying these alterations are largely unknown, but intracellular calcium (Ca2+) overload, a general feature in models diseases, suggested to play key role pathogenesis. Here we describe massive regulation Ca2+ responsive genes sCJD brain...