A5025418255- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Protein Degradation and Inhibitors
- Fungal and yeast genetics research
- Phagocytosis and Immune Regulation
- Genetic and rare skin diseases.
- Innovation and Socioeconomic Development
- COVID-19 Clinical Research Studies
- Bioinformatics and Genomic Networks
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- CAR-T cell therapy research
- Intellectual Property and Patents
- RNA modifications and cancer
- Racial and Ethnic Identity Research
- 3D Printing in Biomedical Research
- Animal Virus Infections Studies
- Bacterial Genetics and Biotechnology
GlaxoSmithKline (United States)
2018-2024
University of Nevada, Las Vegas
2021
University of California, Berkeley
2009-2014
National Institutes of Health
2007-2009
National Institute of Allergy and Infectious Diseases
2007-2009
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign at specific sites. We show here that Cas9 assembles with hybrid guide RNAs human cells and can induce the formation of double-strand breaks (DSBs) site complementary RNA sequence genomic DNA. This cleavage activity requires both binding RNA. Experiments using extracts from transfected expression and/or assembly into is limiting factor for Cas9-mediated cleavage. In addition, we find...
No single animal model for severe acute respiratory syndrome (SARS) reproduces all aspects of the human disease. Young inbred mice support SARS-coronavirus (SARS-CoV) replication in tract and are available sufficient numbers statistical evaluation. They relatively inexpensive easily accessible, but their use SARS research is limited because they do not develop illness following infection. Older (12- to 14-mo-old) BALB/c clinical pneumonitis, can be hard procure, immune senescence complicates...
Clathrin-mediated endocytosis (CME) involves the recruitment of numerous proteins to sites on plasma membrane with prescribed timing mediate specific stages process. However, how choreographed and function during CME is achieved remains unclear. Using genome editing express fluorescent fusion at native levels live-cell imaging single-molecule sensitivity, we explored dynamin2 stoichiometry, dynamics, functional interdependency actin. Our quantitative analyses revealed heterogeneity in early...
ABSTRACT The genome of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains eight open reading frames (ORFs) that encode novel proteins. These accessory proteins are dispensable for in vitro and vivo replication thus may be important other aspects virus-host interactions. We investigated functions largest proteins, ORF 3a protein, using a 3a-deficient strain SARS-CoV. Cell death Vero cells after infection with SARS-CoV was reduced upon deletion 3a. Electron...
Recent studies have revealed the detailed timing of protein recruitment to endocytic sites in budding yeast. However, little is understood about early stages their formation. Here we identify septin-associated Syp1p as a component machinery that drives clathrin-mediated endocytosis arrives at formation and shares unique dynamics with EH-domain Ede1p. We find related amino acid sequence several mammalian proteins one which, SGIP1-α, an binds Ede1p homolog Eps15. Like Syp1p, SGIP1-α...
Src homology 3 (SH3) domains bind peptides to mediate protein–protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, worm Caenorhabditis elegans , discovered it structurally mirrors budding yeast Saccharomyces cerevisiae . then mapped interactome stringent two‐hybrid compared with equivalent map for yeast. found resembles analogous network because is significantly enriched...
We developed a general approach for investigation of how cellular processes become adapted specific cell types during differentiation. Previous studies reported substantial differences in the morphology and dynamics clathrin-mediated endocytosis (CME) sites. However, associating CME properties with distinct differentiated determining these are developmentally specified differentiation have been elusive. Using genome-edited human embryonic stem cells, isogenic fibroblasts neuronal progenitor...
Abstract Tumors in patients non-responsive to immunotherapy harbor a series of barriers that impede the efficacy effector T-cells. Consequently, therapeutically modulating chemotaxis machinery enable T cell infiltration and function tumor could result more successful therapeutic outcomes. Complex in-vitro models allow re-creation in-vivo complexities an setting, allowing improved translatability patient biology at laboratory scale. We identified gap available industrial scale...
CRISPR/Cas9–based gene knockouts (KOs) enable precise perturbation of target function in human cells, which is ideally assessed an unbiased fashion by molecular omics readouts. Typically, this requires the lengthy process isolating KO subclones. We show here that subclones are phenotypically heterogenous, regardless guide RNA used. present experimental strategy avoids subcloning and achieves fast efficient silencing on cell pools, based synergistic combination two RNAs mapping at close...
Malfunctions in the basic epigenetic mechanisms such as histone modifications, DNA methylation, and chromatin remodeling are implicated a number of cancers immunological neurodegenerative conditions. Within GlaxoSmithKline (GSK) we have utilized variations NanoBRET technology for direct measurement compound-target engagement within native cellular environments to drive high-throughput, routine structure-activity relationship (SAR) profiling across differing targets. is variation...
Microaggressions are typically brief and common behavioral/verbal indignities that communicate a sense of hostility, derogatory, or negative perception towards targeted group. During the COVID-19 pandemic, we observed an increase in amount microassaults, more intentional type microaggression, Asian American population. In our study, used two-way 2x2 (Race: Americans vs. White Americans; Time: before during COVID-19) analysis covariance (ANCOVA) with repeated measures on one factor (Time) to...
Introduction: Porokeratosis is a group of rare skin diseases that commonly present as brownish-red blanching papules with well-defined, keratinized border. While most cases are idiopathic, porokeratosis lesions believed to be formed from the expansion abnormal epidermal keratinocytes activated by stressors such infection, UV-irradiation, and immunosuppression. Most asymptomatic, but Eruptive disseminated (EDP), an infrequent variant, can pruritic has higher incidence relapse. EDP also...