A5044226383- interferon and immune responses
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Hemophilia Treatment and Research
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Acute Kidney Injury Research
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Heme Oxygenase-1 and Carbon Monoxide
- Thermal Regulation in Medicine
- Hemostasis and retained surgical items
- Influenza Virus Research Studies
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Biomedical and Engineering Education
- COVID-19 Clinical Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Phagocytosis and Immune Regulation
- Traumatic Brain Injury and Neurovascular Disturbances
Hôpital Maisonneuve-Rosemont
2025
Université de Montréal
2024-2025
Université du Québec à Trois-Rivières
2016-2018
Iranian Legal Medicine Organization
2009-2010
Lipocalin-2 (Lcn2, NGAL) is a member of the lipocalin super family with diverse function such as induction apoptosis, suppression bacterial growth, and modulation inflammatory response. Much interest has recently been focused on physiological/pathological role lipocalin-2 that considered to be novel protective factor against oxidative stress. However, its precise biological roles in this protection are not fully understood. In report we intended test effect expression heme oxygenase ((1, 2))...
Severe COVID-19 can trigger a cytokine storm, leading to acute respiratory distress syndrome (ARDS) with similarities superantigen-induced toxic shock syndrome. An outstanding question is whether SARS-CoV-2 protein sequences directly induce inflammatory responses. In this study, we identify region in the S2 spike sequence homology bacterial super-antigens (termed P3). Computational modeling predicts P3 binding sites on MHC class I/II and TCR that partially overlap for of staphylococcal...
The promyelocytic leukemia (PML) protein, a type I interferon (IFN-I)-induced gene product and member of the tripartite motif (TRIM) family, modulates transcriptional activity viruses belonging to various families. Whether PML has an impact on replication HIV-1 not been fully addressed, but recent studies point its possible involvement in restriction human cells maintenance latency cell lines which is constitutively repressed. We investigated further related lentivirus, SIVmac, by murine...
Elite controllers (ECs) are a rare subset of HIV-1 slow progressors characterized by prolonged viremia suppression. HLA alleles B27 and B57 promote the cytotoxic T lymphocyte (CTL)-mediated depletion infected cells in ECs, leading to emergence escape mutations viral capsid (CA). Whether those modulate CA detection innate sensors effectors is poorly known. Here, we investigated targeting from B27/B57+ individuals cytosolic antiviral factors Mx2 TRIM5α. Toward that aim, constructed chimeric...
The type I interferon (IFN-I)-inducible human restriction factor TRIM5α inhibits the infection of cells by specific nonhuman retroviruses, such as N-MLV and EIAV, but does not generally target HIV-1. However, introduction two aminoacid substitutions, R332G R355G, in (huTRIM5α) domain responsible for retroviral capsid recognition leads to efficient HIV-1 upon stable over-expression. CRISPR-Cas-based approaches precisely edit DNA could be employed modify TRIM5 cells. Toward this aim, we used a...
Factor VII (FVII) is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. It converted factor VIIa plays an important role cascade. The aim this study was isolating and cloning genes human hepsin subsequent co-transfection constructs Chinese hamster ovary (CHO) cell line obtain rFVIIa.Factor cDNAs were isolated from HepG2 cloned into pcDNA3.1 (+) vector. co-transfected CHO line. A permanently expressed recombinant (rFVII) established. expression...
The PML (promyelocytic leukemia) protein is a member of the TRIM family, large group proteins that show high diversity in functions but possess common tripartite motif giving family its name. We and others recently reported both murine (mPML) human (hPML) strongly restrict early stages infection by HIV-1 other lentiviruses when expressed mouse embryonic fibroblasts (MEFs). This restriction activity was found to contribute type I interferon (IFN-I)-mediated inhibition MEFs. Additionally,...
Factor VII (FVII) is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. The aim this study was construct, express and purify recombinant FVII fused polyhistidine (his) tag using Gateway technology.To construct entry clone, blunt-end cDNA subsequent polymerase chain reaction (PCR) product isolated from HepG2 cell line TOPO-cloned into pENTR TOPO vector. To expression LR recombination carried out between clone destination vector, pDEST26....
Severe COVID-19 can trigger a cytokine storm, leading to acute respiratory distress syndrome (ARDS) with similarities superantigen-induced toxic shock syndrome. An outstanding question is whether SARS-CoV-2 protein sequences directly induce inflammatory responses. In this study, we identify region in the S2 spike sequence homology bacterial super-antigens (termed P3). Computational modeling predicts P3 binding sites on MHC class I/II and TCR that partially overlap for of staphylococcal...
Abstract The PML (promyelocytic leukemia) protein is a member of the TRIM family, large group proteins that show high diversity in functions but possess common tripartite motif giving family its name. We and others recently reported both murine (mPML) human (hPML) strongly restrict early stages infection by HIV-1 other lentiviruses when expressed mouse embryonic fibroblasts (MEFs). This restriction activity was found to contribute type I interferon (IFN-I)-mediated inhibition MEFs....