A5100400373- Biosensors and Analytical Detection
- Biomedical Ethics and Regulation
- CRISPR and Genetic Engineering
- Clostridium difficile and Clostridium perfringens research
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- bioluminescence and chemiluminescence research
- Animal Behavior and Welfare Studies
- Cell Image Analysis Techniques
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- RNA and protein synthesis mechanisms
- Gut microbiota and health
Southeast University
2017-2021
State Key Laboratory of Digital Medical Engineering
2017-2021
Huazhong Agricultural University
2020
Institute of Zoology
2015
Chinese Academy of Sciences
2015
Abstract Introduction Human induced pluripotent stem cells (hiPSCs) are considered as one of the most promising seed cell sources in regenerative medicine. Now hiPSC-based clinical trials underway. To ensure safety, used or therapies should be generated under GMP conditions, and with Xeno-free culture media to avoid possible side effects like immune rejection that by Xeno reagents. However, up now there no reports for hiPSC lines developed completely conditions using Methods Clinical-grade...
Early intervention with fecal microbiota transplantation (FMT) improves the growth performance and intestinal barrier function of piglets. Accelerating oxygen concentration is beneficial for symbiotic bacterial colonization. Saccharomyces boulardii (SB) an aerobic fungus, which may contribute to colonization anaerobic bacteria by competing oxygen. Clostridium butyricum (CB) performance, via regulating gut composition The objective this study was investigate effect early FMT combining CB SB...
We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific and ddNTP-blocked primers. To validate its feasibility, sets products, including SNPs, UGT1A1⁎6 (rs4148323) UGT1A1⁎28 (rs8175347), K1637K (rs11176013) S1647T (rs11564148), were analyzed. Haplotypes UGT1A1⁎28, successfully analyzed by Sanger Also, haplotypes S1647T, could not be determined primers but pyrosequencing As result, this...
In the present study, we developed a novel digital coding combination analysis (DCCA) to analyze gene mutation based on sample principle. The principle is that any numerically named divided into two groups, samples are not grouped in same and can be tested within detection limit. Therefore, proposed specific N were M groups. Then analyzed, which could obtain results of mixed If only groups showed positive (mutant type) signals, number from signal would sample, remaining negative (wild type)....