A5050921746- Multiple Myeloma Research and Treatments
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Chemokine receptors and signaling
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Helicobacter pylori-related gastroenterology studies
- Tissue Engineering and Regenerative Medicine
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Sarcoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Clostridium difficile and Clostridium perfringens research
- vaccines and immunoinformatics approaches
- Renal Diseases and Glomerulopathies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Clinica Universidad de Navarra
2020-2025
Navarre Institute of Health Research
2020-2025
Universidad de Navarra
2020-2025
Centro de Investigación Biomédica en Red de Cáncer
2020-2024
Hospital Universitario La Paz
1992
Abstract While myelodysplastic syndromes with del(5q) (del(5q) MDS) comprises a well-defined hematological subgroup, the molecular basis underlying its origin remains unknown. Using single cell RNA-seq (scRNA-seq) on CD34 + progenitors from MDS patients, we have identified cells harboring deletion, characterizing transcriptional impact of this genetic insult disease pathogenesis and treatment response. Interestingly, both non-del(5q) present similar lesions, indicating that all cells, not...
Abstract Generation of upscaled quantities human‐induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CM), for therapeutic or testing applications, is both expensive and time‐consuming. Herein, a scalable bioprocess hiPSC‐CM expansion in stirred‐tank bioreactors (STB) developed. By combining the continuous activation Wnt pathway, through perfusion CHIR99021, within mild hypoxia environment, as aggregates maximized, reaching 4 billion pure 2L STB. In particular, importance i)...
ABSTRACT The bone marrow (BM) microenvironment plays a crucial role in regulating hematopoiesis, yet the molecular and functional changes associated with aging humans remain poorly understood. Using single-cell RNA sequencing (scRNA-seq), we uncovered transcriptional shifts BM endothelial cells (EC) mesenchymal stem (MSC) during aging. Our analysis revealed that aged sinusoidal EC adopt prothrombotic, exhibit mitochondrial dysfunction, have compromised vascular function. Additionally,...
ABSTRACT The role of the bone marrow microenvironment (BME) in transition from monoclonal gammopathy undetermined significance (MGUS) into clinically active multiple myeloma (MM) is not completely determined. To address this issue, we performed single-cell RNA sequencing (scRNA-seq) non-hematopoietic BME cells as well plasma (PC) two genetically engineered mouse models MM termed BI cγ1 and MI that recapitulate progression MGUS MM. Our results identify distinct transcriptional dynamics...
ABSTRACT The bone marrow (BM) is a complex tissue where spatial relationships influence cell behavior, signaling, and function. Consequently, understanding the whole dynamics of cellular interactions requires complementary techniques that preserve map architecture populations in situ . We successfully conducted transcriptional profiling using Visium Spatial Gene Expression formalin-fixed paraffin-embedded (FFPE) BM samples obtained from healthy Multiple Myeloma (MM) mouse models patients,...
Patients with myeloid neoplasms who relapsed after allogenic hematopoietic stem cell transplant (HSCT) have poor prognosis. Monitoring of chimerism and specific molecular markers as a surrogate measure relapse is not always helpful; therefore, improved systems to detect early are needed. We hypothesized that the use next generation sequencing (NGS) could be suitable approach for personalized follow-up post-HSCT. To validate our hypothesis, we analyzed by NGS, retrospective set peripheral...
Clonal evolution in acute myeloid leukemia (AML) originates long before diagnosis and is a dynamic process that may affect survival. However, it remains uninvestigated during routine diagnostic workups. We hypothesized the mutational status of bone marrow dysplastic cells leukemic blasts, analyzed at onset AML using integrated multidimensional flow cytometry (MFC) immunophenotyping fluorescence-activated cell sorting (FACS) with next-generation sequencing (NGS), could reconstruct...
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ABSTRACT While del(5q) MDS patients comprise a well-defined hematological subgroup, the molecular basis underlying its origin, and reason behind relapse after lenalidomide remains unknown. Using scRNA-seq on CD34 + progenitor cells from with we were able to identify harboring deletion, enabling us deeply characterize transcriptional impact of this genetic insult disease pathogenesis treatment response. We found, across all patients, an enrichment in GMP megakaryocyte-erythroid progenitors...