A5100428584- Microbial Metabolic Engineering and Bioproduction
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Bioenergy crop production and management
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Forest Biomass Utilization and Management
- Circular RNAs in diseases
- Agricultural and Food Sciences
- Plant biochemistry and biosynthesis
- DNA and Biological Computing
- Ferroptosis and cancer prognosis
- Agriculture, Land Use, Rural Development
- Wnt/β-catenin signaling in development and cancer
- Gene Regulatory Network Analysis
- Receptor Mechanisms and Signaling
- Agriculture and Rural Development Research
- Protein Kinase Regulation and GTPase Signaling
- Agricultural Economics and Policy
- Microbial metabolism and enzyme function
- Genetic diversity and population structure
Chengdu University of Traditional Chinese Medicine
2024
École Polytechnique
2023
Centre National de la Recherche Scientifique
2023
Central South University
2023
Xiangya Hospital Central South University
2023
Harbin Medical University
2022
Third Affiliated Hospital of Harbin Medical University
2022
Hebei Medical University
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Chinese Academy of Sciences
2014-2021
Abstract Cepharanthine is a secondary metabolite isolated from Stephania . It has been reported that it anti-conronaviruses activities including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we assemble three genomes ( S. japonica , yunnanensis and cepharantha ), propose the cepharanthine biosynthetic pathway, assess antiviral potential of compounds involved in pathway. Among genomes, near telomere-to-telomere assembly with one remaining gap, have chromosome-level...
Abstract Mentha is a commonly used spice worldwide, which possesses medicinal properties and fragrance. These characteristics are conferred, at least partially, by essential oils such as menthol. In this study, gap-free assembly with genome size of 414.3 Mb 31,251 coding genes was obtained for suaveolens ‘Variegata’. Based on its high heterozygosity (1.5%), two complete haplotypic assemblies were resolved, sizes 401.9 405.7 Mb, respectively. The telomeres centromeres each haplotype almost...
Abstract Modified nucleotides in non-coding RNAs, such as tRNAs and snRNAs, represent an important layer of gene expression regulation through their ability to fine-tune mRNA maturation translation. Dysregulation modifications the enzymes installing them have been linked various human pathologies including neurodevelopmental disorders cancers. Several methyltransferases (MTases) are regulated allosterically by TRMT112 (Trm112 Saccharomyces cerevisiae), but interactome this regulator targets...
Most RNAs and many protein factors involved in mRNA maturation translation are decorated by numerous diverse chemical modifications, which contribute to the efficiency, fidelity regulation of these complex essential cellular processes. Among those methylation catalyzed mainly S-adenosyl-L-methionine (SAM) dependent methyltransferases (MTases) is most common one. TRMT112 a small acting as an allosteric regulator several MTases. Initial studies focusing on its associated MTases were performed...
In forensic science, accurate estimation of the age a victim or suspect can facilitate investigators to narrow search and aid in solving crime. Aging is complex process associated with various molecular regulations on DNA RNA levels. Recent studies have shown that circular RNAs (circRNAs) upregulate globally during aging multiple organisms such as mice C.elegans because their ability resist degradation by exoribonucleases. current study, we attempted investigate circRNAs' potential...
// Qing Shao 1, * , Xinrong Luo 2, Dejuan Yang 1 Can Wang Qiao Cheng 2 Tingxiu Xiang Guosheng Ren Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital Medical University, Chongqing, China Department Endocrine Breast Surgery, These authors have contributed equally to this work Correspondence to: Xiang, email: larissaxiang@163.com Ren, rengs726@126.com Keywords: PLCD1, tumour suppressor, breast cancer, KIF3A, MMP7 Received: December 15, 2016 Accepted:...
Microbial oil is one important bio-product for its function in energy, chemical, and food industry. Finding suitable substrates key issue industrial application. Both hydrophilic hydrophobic can be utilized by oleaginous microorganisms with two different bio-pathways ("de novo" lipid fermentation "ex fermentation). To date, most of the research on has focused mainly only pathway little work was carried out both "de simultaneously; thus, advantages cannot fulfilled comprehensively. In this...
Significance ICF4 is an inherited disease with early mortality due to immunodeficiency. Although the genetic cause known be a mutation in Lsh (lymphoid-specific helicase) gene, it remains unknown why patients suffer from impaired immune response. Using conditional knockout mouse models, we provide evidence that has hematopoietic cell-intrinsic role promoting B cell development and deficient cells fail conduct step of DNA end joining required yield variety immunoglobulin isotypes during class...
Synaptic vesicle fusion is mediated by an assembly of soluble N-ethylmaleimide-sensitive protein attachment receptors (SNAREs), composed syntaxin 1, NSF-attachment (SNAP)-25, and synaptobrevin-2/VAMP-2. Previous studies have suggested that over-exposure to manganese (Mn) could disrupt synaptic influencing SNARE complex formation, both in vitro vivo. However, the mechanisms underlying this effect remain unclear. Here we employed calpeptin, inhibitor calpains, along with a lentivirus vector...
In this work, corncob acid hydrolysate and its simulated medium whose sugar composition was the same as were used fermentation substrate for lipid production by oleaginous yeast Trichosporon dermatis. On hydrolysate, after 7 days of fermentation, biomass, content, yield, coefficient T. dermatis 17.3 g/L, 40.2%, 7.0 16.5%, respectively. Interestingly, during on glucose, xylose, arabinose, even acetic could be well utilized carbon sources Surprisingly, yield (7.0 g/L) much higher than that...
Ubiquitin-binding enzyme E2T (UBE2T), a member of the E2 family ubiquitin-proteasome pathway, is associated with tumorigenesis varioustumours; however, its role and mechanism in ovarian cancer remain unclear.Our study revealed that UBE2T highly expressed cancer; this high expression was closely related to poor prognosis. Immunohistochemistry used validate cancer. This first demonstrate higher BRCA mutation. Moreover, we demonstrated gene silencing significantly inhibited cell proliferation...
Elephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant was investigated for the first time. During fermentation process hydrolysate, glucose, xylose, and arabinose could be well utilized as carbon sources T. cutaneum. Interestingly, xylose almost no use before glucose consumed completely. This illustrated that simultaneous saccharification did not occur...
Histone modifications have been widely elucidated to play vital roles in gene regulation and cell identity. The Roadmap Epigenomics Consortium generated a reference catalog of several key histone across >100s human types tissues. Decoding these epigenomes into functional regulatory elements is challenging task computational biology. To this end, we adopted differential chromatin modification analysis framework comprehensively determine characterize type-specific (CSREs) their codes the five...
Uterine corpus endometrial carcinoma (UCEC) is the fourth most common cancer among women worldwide. The 5 year survival rate for patients with advanced UCEC only 17%. Lysine-specific demethylase 4B (KDM4B), a histone demethylase, overexpressed or dysregulated in various cancers and associated tumor progression poor prognosis. We performed bioinformatics analysis vitro assays to assess role of KDM4B UCEC. Additionally, immune microenvironment was explored.The mRNA protein levels were...
Lumping kinetics models were built for the biological treatment of acetone–butanol–ethanol (ABE) fermentation wastewater by oleaginous yeast Trichosporon cutaneum with different temperatures. Compared high temperature (33°C, 306 K) and low (23°C, 296 K), medium (28°C, 301 was beneficial cell growth chemical oxygen demand (COD) degradation during early stage but final biomass COD removal influenced little. By lumping method, materials in bioconversion network divided into five lumps (COD,...
Anisodus acutangulus (Solanaceae), an important folk medicinal herb in China, produces up to 1.2% alkaloids more than that other Solanaceae plants such as Hyoscyamus niger, while its evolutionary position Hyoscyameae is not very clear. To explain the of A. via complete chloroplast genome(cp) sequence. Complete genome was obtained and characterized using Illumina PE150 pair-end sequencing data. Structure genome, codon usage, nucleotide variability (Pi) value, distribution repeats SSRs between...