A5060206984- Mitochondrial Function and Pathology
- Pharmacological Effects of Natural Compounds
- Climate Change and Health Impacts
- Air Quality and Health Impacts
- Corneal Surgery and Treatments
- Coenzyme Q10 studies and effects
- Advanced Glycation End Products research
- Connexins and lens biology
- Immune Response and Inflammation
- Ocular Surface and Contact Lens
- Retinal Diseases and Treatments
- Glutathione Transferases and Polymorphisms
- Medical and Biological Ozone Research
- Natural product bioactivities and synthesis
- Phytochemistry and biological activity of medicinal plants
- Retinal Development and Disorders
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Ocular Infections and Treatments
- Phytochemistry and Bioactivity Studies
- Redox biology and oxidative stress
- Food Quality and Safety Studies
- Acne and Rosacea Treatments and Effects
- Nuclear Receptors and Signaling
- Calcium signaling and nucleotide metabolism
Wayne State University
2016-2024
Michigan United
2016
University of Windsor
2004-2005
Abstract Thioredoxin-interacting protein (TXNIP) is involved in oxidative stress and apoptosis diabetic retinopathy. However, the role of TXNIP removal damaged mitochondria (MT) via mitophagy, a process macroautophagy, remains unexplored. Here we investigate associated cellular molecular mechanisms underlying mitophagy retinal cells under conditions. For this, maintained rat Müller cell line (rMC1) high-glucose (25 mM, HG) or low-glucose (5.5 LG) condition for 5 days. Our data reveal that HG...
Coiled-coil-helix-coiled-coil-helix domain–containing 10 (CHCHD10) and CHCHD2 (MNRR1) are homologous proteins with 58% sequence identity belong to the twin CX9C family of that mediate cellular stress responses. Despite identification several neurodegeneration-associated mutations in CHCHD10 gene, few studies have assessed its physiological role. Here, we investigated CHCHD10's function as a regulator oxidative phosphorylation mitochondria nucleus. We show copurifies cytochrome c oxidase...
Thioredoxin-interacting protein (TXNIP) plays a critical role in oxidative stress, inflammation, apoptosis and the pathogenesis of diabetic retinopathy (DR). However, TXNIP high glucose-induced retinal pigment epithelium (RPE) dysfunction is still unknown. Here, we show that glucose (HG; 25 mM,) significantly increases expression at both mRNA levels when compared to low (LG; 5.5 mM) human RPE cell line (ARPE-19) primary (HRPE) cells. upregulation associated with mitochondrial membrane...
We have previously shown that PM10 exposure causes oxidative stress and reduces Nrf2 protein levels, SKQ1 pre-treatment protects against this damage in human corneal epithelial cells (HCE-2). The current study focuses on uncovering the mechanisms underlying acute toxicity SKQ1-mediated protection. HCE-2 were pre-treated with then exposed to 100 μg/mL PM10. Cell viability, markers, programmed cell death, DNA damage, senescence pro-inflammatory cytokines analyzed. cellular location its...
To determine the effects of airborne particulate matter (PM) <2.5 µm in vitro and on normal Pseudomonas aeruginosa (PA)-infected cornea.An MTT viability assay tested PM2.5 mouse corneal epithelial cells (MCEC) human (HCET). MCEC were for reactive oxygen species using a 2',7'-dichlorodihydrofluorescein assay; RT-PCR determined mRNA levels inflammatory oxidative stress markers (HMGB1, toll-like receptor 2, IL-1β, CXCL2, GPX1, GPX2, GR1, superoxide dismutase heme oxygenase 1) HCET (high...
The purpose of this study is to test the effects whole-body animal exposure airborne particulate matter (PM) with an aerodynamic diameter <10 μm (PM10) in mouse cornea and vitro. C57BL/6 mice were exposed control or 500 µg/m3 PM10 for 2 weeks. In vivo, reduced glutathione (GSH) malondialdehyde (MDA) analyzed. RT-PCR ELISA evaluated levels nuclear factor erythroid 2-related (Nrf2) signaling inflammatory markers. SKQ1, a novel mitochondrial antioxidant, was applied topically GSH, MDA Nrf2...
Purpose: To test how glycyrrhizin (GLY) affects mouse corneal epithelial cells (MCEC) and the diabetic murine cornea. Methods: Viability of MCEC grown under normal or high glucose (HG) with/without GLY was tested by an MTT assay. In addition, C57BL/6 mice were injected with streptozotocin a subset control received in their drinking water. mRNA protein levels proinflammatory oxidative stress molecules reverse transcription-polymerase chain reaction (RT-PCR) both models. Ex vivo studies using...
Purpose In vivo data indicate that mouse corneas exposed to PM 10 showed early perforation and thinning after infection with Pseudomonas aeruginosa . To understand the mechanisms underlying this finding, we tested effects of mitochondria targeted anti-oxidant SKQ1 in immortalized human corneal epithelial cells (HCET) were challenged strain 19660. Methods Mouse infected 19660 a 2 week whole-body exposure or control air assessed by clinical scores, slit lamp photography western blot. HCET...
The conserved miR-183/96/182 cluster (miR-183C) is expressed in both corneal resident myeloid cells (CRMCs) and sensory nerves (CSN) modulates immune/inflammatory responses. To uncover cell type-specific roles of miR-183C CRMC CSN their contributions to physiology, myeloid-specific conditional knockout (MS-CKO), nerve-specific CKO (SNS-CKO) mice were produced characterized comparison the conventional KO. Immunofluorescence confocal microscopy flatmount corneas, sensitivity, tear volume...
The effects of glycyrrhizin (GLY) on multi-drug resistant (MDR) systemic (MDR9) vs. ocular (B1045) Pseudomonas aeruginosa clinical isolates were determined. Proteomes each isolate with/without GLY treatment profiled using liquid chromatography mass spectrometry (LC-MS/MS). effect adherence MDR to immortalized human (HCET) and mouse (MCEC) corneal epithelial cells, biofilm dispersal was tested. Both treated with (0.25 minimum inhibitory concentration (MIC), 10 mg/mL for MDR9 3.75 B1045)...
Abstract The conserved miR-183/96/182 cluster (miR-183C) is expressed in both corneal resident myeloid cells (CRMCs) and sensory nerves (CSN) modulates immune/inflammatory responses. To uncover cell type-specific roles of miR-183C CRMC CSN their contribute to physiology, myeloid-specific conditional knockout (MS-CKO), nerve-specific CKO (SNS-CKO) mice were produced characterized comparison the conventional KO. Immunofluorescence confocal microscopy flatmount corneas, sensitivity, tear volume...
Purpose To test the effects of acidic vs . neutral pH glycyrrhizin (GLY) on unwounded and wounded normal mouse cornea after infection with Pseudomonas aeruginosa isolates KEI 1025 multidrug-resistant MDR9. Methods Acidic or GLY phosphate-buffered saline (PBS) was topically applied to corneas C57BL/6 mice. In corneas, goblet cells corneal nerves were stained quantitated. After wounding, fluorescein photographed using a slit lamp. Mice also infected MDR9 protective evaluated comparatively....
This study tests the mechanism(s) of glycyrrhizin (GLY) protection against P. aeruginosa keratitis. Female C57BL/6 (B6), TLR4 knockout (TLR4KO), myeloid specific TLR4KO (mTLR4KO), their wildtype (WT) littermates, and TLR9 (TLR9KO) mice were infected with KEI 1025 treated GLY or PBS onto cornea after infection. Clinical scores, photography a slit lamp, RT-PCR ELISA used. effects on macrophages (Mϕ) polymorphonuclear neutrophils (PMN) isolated from WT mTLR4KO challenged also tested. Comparing...