A5018854753- FOXO transcription factor regulation
- Metabolism, Diabetes, and Cancer
- Advanced Cellulose Research Studies
- TGF-β signaling in diseases
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Genomics, phytochemicals, and oxidative stress
- Heat shock proteins research
- Adipose Tissue and Metabolism
- Glutathione Transferases and Polymorphisms
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Redox biology and oxidative stress
- Polysaccharides and Plant Cell Walls
- Macrophage Migration Inhibitory Factor
- Ubiquitin and proteasome pathways
- Phytochemicals and Antioxidant Activities
- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Biofuel production and bioconversion
- Pharmacological Effects of Natural Compounds
- Enzyme-mediated dye degradation
- Protein Degradation and Inhibitors
- Platelet Disorders and Treatments
Chungbuk National University
2016-2025
Life University
2023
Rural Development Administration
2023
Cheongju University
2014
p53 plays a critical role in variety of growth inhibitory responses, including cell cycle arrest, differentiation, and apoptosis, contributes to tumor suppression. Here we show that NM23-H1 its binding partner STRAP (serine-threonine kinase receptor-associated protein) interact with potentiate activity. Both directly the central DNA domain within residues 113-290. The use mutants revealed Cys(145) Cys(152) (or Cys(270)) were responsible for binding. Furthermore, Cys(176) Cys(135) required...
Murine protein serine/threonine kinase 38 (MPK38) is a member of the AMP-activated kinase-related family that plays an important role in various cellular processes, including cell cycle, signaling pathways, and self-renewal stem cells. Here we demonstrate functional association between MPK38 apoptosis signal-regulating 1 (ASK1). The physical ASK1 was mediated through their carboxyl-terminal regulatory domains increased by H2O2 or tumor necrosis factor α treatment. use kinase-dead mutants...
This study was conducted to investigate the effect of dietary oleic acid in olive oil-supplemented diets on blood lipid profile and fatty composition plasma adipose tissue rats. A total 60 Sprague Dawley rats with mean body weight 249 g ± 3.04 were equally divided into three diet groups: control (CON) contained 10% coconut oil, olive50 5% oil olive100 oil. Oleic (OA) level highest followed by control. The final (BW) significantly affected intake OA, which fed had lowest BW, signified that OA...
Background/Objectives: Regulation of acute inflammatory responses is crucial for host mortality and morbidity induced by pathogens. The pathogenesis respiratory distress syndrome (ARDS) sepsis are associated with systemic inflammation. p38 MAPK a regulator potential target diseases, including ARDS sepsis. We investigated the therapeutic effects TAT-TN13 peptide (TN13) on severe sepsis, in vivo. Methods: To establish model, C57BL/6 mice were intranasally (i.n.) administered lipopolysaccharide...
Small heterodimer partner (SHP; NR0B2) is an atypical orphan nuclear receptor and acts as a coregulator of various receptors. Herein, we examined novel cross talk between SHP forkhead transcription factor HNF3 (hepatocyte 3/Foxa. Transient transfection assay demonstrated that inhibited the transcriptional activity all three isoforms HNF3, HNF3α, β, γ. In vivo in vitro protein interaction studies showed physically interacted with HNF3. Adenovirus-mediated overexpression significantly...
NM23-H1 is a member of the NM23/NDP kinase gene family and putative metastasis suppressor. Previously, screen for NM23-H1-interacting proteins that could potentially modulate its activity identified serine-threonine receptor-associated protein (STRAP), transforming growth factor (TGF)-β receptor-interacting protein. Through use cysteine to serine amino acid substitution mutants (C4S, C109S, C145S) STRAP (C152S, C270S, C152S/C270S), we demonstrated association between these two dependent on...
Ganoderma lucidum is used widely in oriental medicine to treat obesity and metabolic diseases. Bioactive substances extracted from G. have been shown ameliorate dyslipidemia, insulin resistance, type 2 diabetes mice via multiple 5′ AMP-activated protein kinase (AMPK)-mediated mechanisms; however, further studies are required elucidate the anti-obesity effects of vivo. In this study, we demonstrated that 3% extract powder (GEP) can be prevent resistance a mouse model. C57BL/6 were provided...
To gain more insights about the biological roles of PDK1, we have used yeast two-hybrid system and in vivo binding assay to identify interacting molecules that associate with PDK1. As a result, serine-threonine kinase receptor-associated protein (STRAP), transforming growth factor-β (TGF-β) receptor-interacting protein, was identified as an partner STRAP found form complexes PDK1 intact cells. Mapping analysis revealed this only mediated by catalytic domain not pleckstrin homology domain....
Abstract Smad proteins have been implicated in metabolic processes, but little is known about how they regulate metabolism. Because 2, 3, 4, and 7 previously shown to interact with murine protein serine–threonine kinase 38 (MPK38), an AMP‐activated (AMPK)-related that has obesity-associated defects, we investigated whether processes via MPK38. Smads2/3/4 increased, Smad7 decreased, MPK38-mediated apoptosis signal-regulating kinase-1 (ASK1)/transforming growth factor-β (TGF-β)/p53 signaling....
Ginsenoside is the primary active substance of ginseng and has many pharmacological effects, such as anti-cancer, immune, regulating sugar lipid metabolism, antioxidant effects. It also protects nervous cardiovascular systems. This study analyzes effects thermal processing on bioactivities crude saponin. Heat treatment increased contents minor ginsenosides in saponins, Rg3, heat-treated saponin (HGS) had better neuroprotective than non-treated (NGS). HGS reduced glutamate-induced apoptosis...
Small heterodimer partner (SHP; NR0B2) is an atypical orphan nuclear receptor that lacks a conventional DNA binding domain (DBD) and represses the transcriptional activity of various receptors. In this study, we examined novel cross talk between SHP BETA2/NeuroD, basic helix-loop-helix transcription factor. vitro in vivo protein interaction studies showed physically interacts with but not its E47. Moreover, confocal microscopic study immunostaining results demonstrated colocalized BETA2...
Macrophage migration inhibitory factor (MIF) is a pluripotent cytokine that involved in host immune and inflammatory responses, as well tumorigenesis. However, the regulatory mechanism of MIF function unclear. Here we report NM23-H1 interacts with cells, demonstrated by cotransfection coimmunoprecipitation experiments. Analysis cysteine (Cys) to serine (Ser) substitution mutants (C4S, C109S, C145S) (C57S, C60S, C81S) revealed Cys(145) Cys(60) are responsible for complex formation....
SHP (small heterodimer partner) is a well-known NR (nuclear receptor) co-regulator. In the present study, we have identified new SHP-interacting protein, termed SMILE (SHP-interacting leucine zipper protein), which was previously designated as ZF (Zhangfei) via yeast two-hybrid system. We determined that gene generates two isoforms [SMILE-L (long isoform of SMILE) and SMILE-S (short SMILE)]. Mutational analysis has demonstrated arise from alternative usage initiation codons. confirmed in...
Serine-threonine kinase receptor-associated protein (STRAP) interacts with transforming growth factor β (TGF-β) receptors and inhibits TGF-β signaling. Here, we identify STRAP as an interacting partner of ASK1 (apoptosis signal-regulating 1). The association between is mediated through the C-terminal domain fourth sixth WD40 repeats STRAP. Using cysteine-to-serine amino acid substitution mutants (C1005S, C1351S, C1360S, C1351S/C1360S) (C152S, C270S, C152S/C270S), demonstrated that Cys1351...
We have reported previously that PDK1 physically interacts with STRAP, a transforming growth factor-β (TGF-β) receptor-interacting protein, and enhances STRAP-induced inhibition of TGF-β signaling. In this study we show coimmunoprecipitates Smad proteins, including Smad2, Smad3, Smad4, Smad7, association is mediated by the pleckstrin homology domain PDK1. The between proteins increased insulin treatment but decreased treatment. Analysis interacting shows enhance kinase activity removing...
Cell survival and death-inducing signals are tightly associated with each other, the decision as to whether a cell survives or dies is determined by controlling relationship between these signals. However, mechanism underlying reciprocal regulation of such remains unclear. In this study, we reveal functional association PDK1 (3-phosphoinositide-dependent protein kinase 1), critical mediator survival, ASK1 (apoptosis signal-regulating an apoptotic stress-activated MAPKKK. The physical...
We have identified previously a new murine protein serine/threonine kinase, MPK38, closely related to the sucrose-non-fermenting kinase family [Gil, Yang, Lee, Choi and Ha (1997) Gene 195, 295–301]. Using C-terminal half of putative human counterpart HPK38, as bait in yeast two-hybrid screen HeLa cDNA library, it was discovered that zinc-finger-motif-containing protein, termed zinc-finger-like 9 (ZPR9), bound both HPK38 MPK38. In co-expression assay, ZPR9 associated with MPK38 vivo, we...
We have identified previously a new murine protein serine/threonine kinase, MPK38, closely related to the sucrose-non-fermenting kinase family [Gil, Yang, Lee, Choi and Ha (1997) Gene 195, 295–301]. Using C-terminal half of putative human counterpart HPK38, as bait in yeast two-hybrid screen HeLa cDNA library, it was discovered that zinc-finger-motif-containing protein, termed zinc-finger-like 9 (ZPR9), bound both HPK38 MPK38. In co-expression assay, ZPR9 associated with MPK38 vivo, we...