Abstract: The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum its antibacterial cytotoxic effects. formation AgNPs was confirmed by color change, it further characterized ultraviolet–visible spectroscopy (435 nm). phytochemical screening C. revealed presence flavonoids, terpenoids, glycosides, suggesting that these compounds act as reducing stabilizing agents. crystalline nature synthesized...

Duration of Positive SARS-CoV-2 on RT-PCR and Culture In 21 consecutive patients with confirmed Covid-19, the median times from symptom onset to negative viral culture real-time RT-PCR...

Genomic copies of three Xanthomonas oryzae pv. avirulence (avr) genes, avrXa7, avrXal0, and avrxa5, four homologous aB3.5, aB3.6, aB4.3, aB4.5, were mutagenized individually or in combination to study the roles avr genes one component pathogen fitness, i.e., aggressiveness amount disease X. causes susceptible rice lines. These are members highly related avrBs3 gene family. Compared wild-type strain, strains with mutations caused shorter lesions on line IR24, which contains no resistance...

This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) tissue samples six (three three cattle) CD29 antibody. Calves' exhibited significantly faster growth rates than did those (P < 0.01), with a doubling time 2.43 days. Genetic analysis revealed higher MyoD Pax7 expression in during proliferation...

This study was conducted to investigate the effect of dietary oleic acid in olive oil-supplemented diets on blood lipid profile and fatty composition plasma adipose tissue rats. A total 60 Sprague Dawley rats with mean body weight 249 g ± 3.04 were equally divided into three diet groups: control (CON) contained 10% coconut oil, olive50 5% oil olive100 oil. Oleic (OA) level highest followed by control. The final (BW) significantly affected intake OA, which fed had lowest BW, signified that OA...

Amaranthus species are widely used as grain and leaf vegetables around the world potential dietary sources of antioxidants phenolic compounds. In this study, we examined variation in total flavonoid contents, polyphenol antioxidant activities among 120 accessions nine species. The activity DPPH (2,2-diphenyl-1-picrylhydrazyl) amaranth ranged from 1.1 (A. tricolor) to 75.2 tricolor mg ascorbic acid equivalents (AAE)/g 2018, 8.5 68.8 dubius) AAE/g 2019. ABTS (2,2′-azinobis...

Genetic polymorphisms have a great impact on enhancing quantitative traits in cattle. In this study, Fatty acid synthase (FASN) g. 16024 (A&gt;G), Stearoyl-CoA desaturase (SCD) 10329 (C&gt;T), and pleomorphic adenoma gene (PLAG1) 25003338 (C&gt;G) genotypic allelic were evaluated, along with their associations fatty composition, adipogenic expression, carcass characteristics (carcass weight, yield grade, backfat thickness, marbling score) Hanwoo steers. A total of 128 steers selected for...

To identify risk factors for nonalcoholic steatohepatitis following pancreaticoduodenectomy, with a focus on related to pancreatic secretions.The medical records of 228 patients who had pancreaticoduodenectomy over 16-mo period were reviewed retrospectively. The 193 did not have fatty liver disease preoperatively included in the final analysis. Hepatic steatosis was diagnosed using differences between splenic and hepatic attenuation liver-to-spleen as measured by non-enhanced computed...

We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous contains the greatest concentration of MUFA and lowest SFA. Therefore, we hypothesized depots would exhibit greater adipogenic gene expression over time than other major depots. Four Angus steers, each at 9, 12, 14, 16 mo age, were harvested fresh samples collected from brisket, chuck, rib, loin, sirloin, round, flank, plate. Relative for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β),...

We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells a co-culture system. Herein we hypothesize saturated fatty acids would adipogenic/lipogenic expression, whereas mono- and polyunsaturated have the opposite effect. Bovine semimembranosus (BSC) intramuscular (IPA) were isolated from crossbred steers cultured with 10% fetal serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) 1% antibiotics during 3-d proliferation...

We evaluated the diagnostic accuracy of two newly developed, point-of-care, rapid antigen tests (RATs) for detecting SARS-CoV-2, AFIAS COVID-19 Ag and ichroma TM Ag, investigated kinetics. A total 200 serially collected nasopharyngeal (NP) specimens from 38 patients 122 negative controls were analyzed. Diagnostic sensitivity specificity assessed in comparison to molecular test results subdivided according targeted genes ( E , RdRP N ) days post-symptom onset (PSO). For kinetics evaluation,...

We hypothesized that supplementing finishing diets with palm oil would promote adipocyte differentiation in subcutaneous adipose tissue of feedlot steers, and soybean supplementation depress differentiation. Twenty-eight Angus steers were assigned randomly to 3 groups 9 or 10 fed a basal diet without additional fat (control), 3% (rich palmitic acid), polyunsaturated fatty acids), for wk, top-dressed daily. Palm had no effect (P > 0.05) on ADG, food intake, G:F, whereas depressed ADG = 0.02),...

The rumen microbial ecosystem is a complex system where fermentation processes involve interactions among microorganisms. There are important relationships between diet and the ruminal bacterial composition. Thus, we investigated characteristics compared communities using tag amplicon pyrosequencing analysis in Yanbian yellow steers, which were fed linseed oil (LO) propionate precursors. We used eight ruminally cannulated steers (510 ± 5.8 kg) replicated 4 × Latin square design with four...

Strains of Xanthomonas oryzae pv. that are virulent to rice lines carrying the Xa21 resistance gene were widely distributed in Korea. A total 105 strains collected during 1987 1996 Korea was characterized by pathogenicity tests and restriction fragment length polymorphism analysis XorII methyltransferase (xorIIM) avrXa10 genes. Although lesion lengths on line IRBB21, which carries Xa21, decreased as plant age increased, susceptibility plants 31 clearly differentiated at seedling (14, 21, 28...

Abstract We hypothesized that oleic acid (OA) in the absence of a thiazolidinedione (i.e., synthetic peroxisome proliferator-activated receptorγ [PPARγ] agonist) would increase adipogenic gene expression bovine muscle satellite cells (BSC). The BSC were cultured differentiation medium containing 10 µM ciglitazone (CI), 100 OA, or OA plus CI (CI-OA). Control (CON) only media (containing 2% horse serum). presence myogenin, desmin, and paired box 7 proteins was confirmed by immunofluorescence...

BACKGROUND: The temporal changes in the Staphylococcus aureus genotypes causing S. bacteremia (SAB) and corresponding clinical over last decade South Korea are rarely investigated. METHODS: A longitudinal study of adult SAB patients was conducted a large referral hospital Seoul, Korea. Adult monomicrobial were enrolled between August 2008 December 2018. Genotyping performed by multilocus sequence typing (MLST) staphylococcal protein (spa) typing. Trends identified linear regression analysis....

Cattle are less active than humans. Hence, it was hypothesized in this study that transmitting acceleration signals at a 1 min sampling interval to reduce storage load has the potential improve performance of motion sensors without affecting precision behavior classification. The classification terms precision, sensitivity, and F1-score serial datasets segmented 3, 4, 5 window sizes based on nine algorithms were determined. collar-fitted triaxial accelerometer sensor attached right side neck...

Abstract We hypothesized that exogenous fatty acids, and especially or 18:2 trans ‐10, cis ‐12 conjugated linoleic acid (CLA), would decrease adipogenic lipogenic gene expression de novo biosynthesis in intramuscular (i.m.) subcutaneous (s.c.) adipose tissues. Fresh i.m. s.c. tissues were collected from the longissimus thoracis muscle of Angus steers at 12, 14, 16 months age ( n = 4 per time point). Adipose tissue explants incubated duplicate for 48 h with 40 μM α‐linolenic (ALA), oleic,...

We hypothesized that fatty acids would differentially affect G protein coupled receptor (GPR) 43 mRNA expression and GPR43 concentrations in bovine intramuscular (IM) subcutaneous (SC) adipocytes. The was detected liver, pancreas, semimembranosus (MUS) muscle samples taken at slaughter. Similarly, levels were similar IM adipose tissue SM but barely detectable SC tissue. Primary cultures of stromal vascular cells isolated from tissues. Oleic acid (100 μ) stimulated PPARγ gene decreased...

We hypothesized that gene expression and fatty acid composition would differ among different muscle depots over time on a grain-based finishing diet. Additionally, we the concentration of SFA decrease MUFA increase proportionately with increases in percent intramuscular lipid (%IML). Ten Yanbian Yellow cattle steers (approximately 8 mo age) were fed corn-based diet which proportion corn ration was increased at 4-mo intervals. Muscle samples taken by biopsy from chuck (trapezius), loin...