A5075775490- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Water resources management and optimization
- Water Systems and Optimization
- Water Treatment and Disinfection
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Groundwater flow and contamination studies
- Wastewater Treatment and Reuse
- Water Quality Monitoring Technologies
- Hydrology and Watershed Management Studies
- Cancer-related molecular mechanisms research
- Water Quality and Pollution Assessment
- Heat shock proteins research
- NF-κB Signaling Pathways
- Flood Risk Assessment and Management
- Molecular Biology Techniques and Applications
- Signaling Pathways in Disease
- Urban Stormwater Management Solutions
- Water-Energy-Food Nexus Studies
- Sensor Technology and Measurement Systems
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
Imperial College London
2025
Duke University
2024-2025
University of Colorado Boulder
2015-2024
Environmental Protection Agency
2009-2024
Yale University
2024
VA Office of Research and Development
2021
United States Department of Homeland Security
2018-2020
KWJ Engineering (United States)
2018
University of Colorado System
2014
Northeastern University
2010
An analysis of the sequence information contained in a compilation published binding sites for E. coli integration host factor (IHF) was performed. The sequences twenty-seven IHF were aligned; base occurrences at each position, content, and an extended consensus obtained site. position site used with program written Apple Macintosh computers order to determine similarity scores sites. A linear correlation found exist between logarithm functional data (relative free energies) two groups...
Problems associated with maintaining and replacing water supply distribution systems are reviewed. Some of these problems public health, economic spatial development the community, costs repair replacement system components. A frequency analysis has been completed for maintenance events (leaks breaks). The implication various strategies effect quality (corrosivity) on loss cost examined. This is based data acquired from tne large (260 mgd; 11.39 m³/s) one smaller (20 0.88 utility. As this...
The basal factor TFIIE is an important component of the RNA polymerase II transcription machinery. In our efforts to determine role in process, we have begun define interactions between and other factors. Here report that binds selectively nonphosphorylated form (IIa) this interaction mediated by 56-kD subunit TFIIE. Additional binding studies reveal TFII can interact with TBP as well TFIID primarily via subunit. Our indicate also interacts both subunits TFIIF TFIIH, a multisubunit reported...
Noncoding RNAs (ncRNAs) are now recognized as transregulators of eukaryotic transcription, a role once attributed exclusively to protein factors. Two ncRNAs in mammalian cells have been shown repress general mRNA transcription by RNA polymerase II (Pol II) response heat shock: mouse B2 and human Alu RNA. bind directly tightly Pol co-occupy the promoters repressed genes along with polymerase. Here, we identified molecular mechanism which transcription. Biochemical assays probe network...
The regulation of histone deacetylases (HDACs) by phosphorylation was examined elevating intracellular in cultured cells with the protein phosphatase inhibitor okadaic acid. After fractionation extracts from treated <i>versus</i> untreated cells, HDAC 1 and 2 eluted several peaks deacetylase activity, assayed using mixed acetylated histones or H4 peptide. Stimulation acid led to hyperphosphorylation as well changes column elution both enzymes. Hyperphosphorylated HDAC2 also observed...
Abstract Sharp increases in fertilizer and pesticide use throughout the 1960s 1970s along with generally less attachment to soil particles may result more widespread contamination of drinking water supplies. The purpose this study was highlight agricultural chemicals their occurrence groundwater while focusing on engineering processes available for removing them acceptable limits consumers. Through various case studies field‐scale research projects, several different treatment technologies...
We previously found that the SINE-encoded mouse B2 RNA binds polymerase II and represses mRNA transcription during cellular heat-shock response. In vitro assembles into preinitiation complexes on promoter DNA via its interaction with polymerase, thus rendering inactive. With goal of understanding which regions are important for high-affinity binding to repression transcription, we performed a structural deletion analysis 178 nucleotide (nt) RNA. describe an experimentally derived secondary...
Central to the development and survival of all organisms is regulation gene expression, which begins with process transcription catalyzed by RNA polymerases. During protein-coding genes, general factors (GTFs) work alongside polymerase II (Pol II) assemble preinitiation complex at start site, open promoter DNA, initiate synthesis nascent messenger RNA, transition productive elongation, ultimately terminate transcription. Through these different stages transcription, Pol dynamically...
Transcription factor TFIID consists of TATA binding protein (TBP) and at least eight TBP-associated factors (TAFs). As TAFs are required for activated but not basal transcription, we have proposed that act as coactivators to mediate signals between activators the transcription machinery. Here report cloning, expression, biochemical characterization 32-kDa subunit human (h) TFIID, termed hTAFII32. We find hTAFII32 is homologue Drosophila TAFII40. In vitro protein-protein interaction assays...