A5012669176- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Iron Metabolism and Disorders
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Ocular Diseases and Behçet’s Syndrome
- Heme Oxygenase-1 and Carbon Monoxide
- Connexins and lens biology
- Systemic Lupus Erythematosus Research
- AI in cancer detection
- Barrier Structure and Function Studies
- Digital Imaging for Blood Diseases
- Diabetes Management and Research
- Vasculitis and related conditions
- Gestational Diabetes Research and Management
- Neurological Complications and Syndromes
- Diabetes Treatment and Management
- Machine Learning in Healthcare
- Augmented Reality Applications
- Pregnancy and preeclampsia studies
- Retinal and Macular Surgery
- Domain Adaptation and Few-Shot Learning
Duke University
2018-2025
Duke Medical Center
2019-2024
University of Puerto Rico at Carolina
2021
Penn Presbyterian Medical Center
2008-2017
University of Pennsylvania
2008-2017
Drexel University
2014-2017
Iron is an essential element in human metabolism but also a potent generator of oxidative damage with levels that increase age. Several studies suggest iron accumulation may be factor age-related macular degeneration (AMD). In prior studies, both overload and features AMD were identified mice deficient the ferroxidase ceruloplasmin (Cp) its homologue hephaestin (Heph) (double knockout, DKO). this study, location timing accumulation, rate reproducibility retinal degeneration, roles stress...
Iron-induced oxidative stress may exacerbate age-related macular degeneration (AMD). Ceruloplasmin/Hephaestin double-knockout (DKO) mice with age-dependent retinal iron accumulation and some features of AMD were used to test protection by the oral chelator deferiprone (DFP).Cultured pigment epithelial (ARPE-19) cells treated DFP. Transferrin receptor mRNA (Tfrc), an indicator levels, was quantified qPCR. In mice, assessed mass spectrometry, histology electroretinography.DFP at 60 μM...
PURPOSE.Iron dysregulation can cause retinal disease, yet iron regulatory mechanisms are incompletely understood.The peptide hormone hepcidin (Hepc) limits uptake from the intestine by triggering degradation of transporter ferroportin (Fpn).Given that Hepc is expressed in retina and Fpn cells constituting blood-retinal barrier, authors tested whether may produce to limit import.METHODS.Retinas Ϫ/Ϫ mice were analyzed histology, autofluorescence spectral analysis, atomic absorption...
Purpose.: To investigate the retinal-protective effects of oral iron chelator deferiprone (DFP) in mice lacking regulatory hormone hepcidin (Hepc). These Hepc knockout (KO) have age-dependent systemic and retinal accumulation leading to degeneration. Methods.: KO were given DFP drinking water from age 6 18 months. They then compared not receiving by fundus imaging, electroretinography (ERG), histology, immunofluorescence, quantitative PCR protective effect against pigment epithelial (RPE)...
Purpose.: The purpose of this study was to investigate light damage–induced transcript changes within neurosensory retina (NSR) and isolated retinal pigment epithelium (RPE). Similar studies have been conducted previously, but were usually limited the NSR only a portion transcriptome. Herein most transcriptome, not just in also RPE, queried. Methods.: Mice exposed 10,000 lux cool white fluorescent for 18 hours euthanized 4 after photic injury. RPE collected, RNA isolated. DNA microarray...
While the availability of public internet-scale datasets images and language has catalyzed remarkable progress in machine learning, medical are constrained by regulations protecting patient privacy time cost required for curation labeling. Self-supervised learning or pretraining demonstrated great success meaningful representations from large unlabeled to enable efficient on downstream tasks. In ophthalmology, RETFound model, a vision transformer (ViT-L) model trained masked autoencoding 1.6...
To investigate the effect of iron chelator deferiprone (DFP) on sodium iodate (NaIO3)-induced retinal degeneration and hereditary caused by rd6 mutation.Retinas from NaIO3-treated C57BL/6J mice, with or without DFP cotreatment, were analyzed histology, immunofluorescence, quantitative PCR to degeneration. facilitate photoreceptor quantification, we developed a new function MATLAB perform this task in semiautomated fashion. Additionally, mice treated histology assess possible protection.In...
Abstract Brain iron accumulates in several neurodegenerative diseases and can cause oxidative damage, but mechanisms of brain homeostasis are incompletely understood. Patients with mutations the cellular iron‐exporting ferroxidase ceruloplasmin ( Cp ) have accumulation causing neurodegeneration. Here, we assessed brains mice combined mutation its homolog hephaestin. Compared to single mutants, was accelerated double mutants cerebellum, substantia nigra, hippocampus. Iron accumulated within...
<h3>Importance</h3> In improving clinical outcomes, developing a sustainable, transformative care delivery model is important for accessible, efficient, low-cost, high-quality community-based imaging and diagnosis of retinal diseases. <h3>Objective</h3> To test the feasibility accuracy remote as screening tool to facilitate identification referable macular degeneration. <h3>Design, Setting, Participants</h3> A nonrandomized study 159 patients was conducted in sites with relatively high...
Hephaestin (Heph) is a ferroxidase protein that converts ferrous to ferric iron facilitate cellular export by ferroportin. Many tissues express either Heph or its homologue, ceruloplasmin (Cp), but the retina expresses both. In mice, combined systemic mutation of and knockout Cp (Cp(-/-), Heph(sla/sla)) causes retinal accumulation degeneration, with features human age-related macular degeneration; however, role in individual cells unclear. Herein, we used conditional mice study Heph's...
Abstract Importance Region‐specific pathology in proliferative diabetic retinopathy enhances our understanding and management of this disease. Background To investigate non‐perfusion, neovascularization macular oedema. Design A cross‐sectional, observational, non‐randomized study. Participants Consecutive 43 eyes 27 treatment‐naïve patients. Methods Ultra‐widefield fluorescein angiography for studying specific zones, that is, far‐peripheral zone, mid‐peripheral zone central retina (cr),...
Giant cell arteritis (GCA) may produce large areas of retinal infarction due to cilioretinal or central/branch artery occlusions. However, focal ischemia in the form cotton-wool spots (CWS) from nonperfusion smaller, more distal vessels also occur GCA, sometimes as an isolated finding (1). Here, we describe another ischemia—paracentral acute middle maculopathy (PAMM)—as a manifestation biopsy-proven GCA. CASE 1 A 75-year-old white woman with history hypertrophic cardiomyopathy and carotid...