A5071777136- Alkaline Phosphatase Research Studies
- Parathyroid Disorders and Treatments
- Pancreatitis Pathology and Treatment
- Heart Failure Treatment and Management
- Bone health and osteoporosis research
- Biochemical and Molecular Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cardiovascular and exercise physiology
- Bone health and treatments
- Neonatal Health and Biochemistry
- Clinical Laboratory Practices and Quality Control
- Metabolism and Genetic Disorders
- Cardiovascular Function and Risk Factors
- Folate and B Vitamins Research
- Pharmacological Effects and Toxicity Studies
- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Diet and metabolism studies
- Cardiac pacing and defibrillation studies
- Adipokines, Inflammation, and Metabolic Diseases
- Transplantation: Methods and Outcomes
- Sepsis Diagnosis and Treatment
- Genomics and Rare Diseases
- BRCA gene mutations in cancer
- Adipose Tissue and Metabolism
University of Antwerp
2013-2024
Antwerp University Hospital
2012-2021
Province of Antwerp
2010
University of Virginia Health System
1991
Adynamic bone disease was recently described to be increasingly prevalent in the dialysis population. At present diagnosis of this type renal osteodystrophy can only made by histomorphometry. We assessed value different biochemical serum markers adynamic disease.In 103 haemodialysis patients a biopsy performed after double tetracycline labelling, and levels intact PTH, osteocalcin, isoenzyme alkaline phosphatase were determined. Bone measured an optimized agarose gel electrophoretic method,...
Adiponectin is an antiinflammatory, insulin-sensitizing, and antiatherogenic adipocytokine that plays a fundamental role in energy homeostasis. In patients with chronic heart failure (CHF), high circulating adiponectin levels are associated inverse outcome. Recently, expression has been identified human skeletal muscle fibers. We investigated the of adiponectin, receptors, genes involved downstream lipid glucose metabolism CHF.Muscle biopsies (vastus lateralis muscle) were obtained from 13...
Because neither the incidence and risk factors for rhabdomyolysis in ICU nor dynamics of its main complication, i.e., rhabdomyolysis-induced acute kidney injury (AKI) are well known, we retrospectively studied a large population adult patients (n = 1,769).CK sMb (serum myoglobin) uMb (urinary were as markers AKI (RIFLE criteria). Hemodialysis mortality used outcome variables.Prolonged surgery, trauma, vascular occlusions associated with increasing CK values. correlates (p < 0.001) peaks...
AimsThis study was designed to evaluate the effects of combined endurance/resistance training on NT-proBNP levels in patients with chronic heart failure (CHF).The safety resistive weight for CHF is questioned. Possible detrimental include an increase ventricular diastolic pressure and secondary unfavourable remodelling. Circulating N-terminal fragment brain natriuretic peptide (NT-proBNP) reflect left wall stress are strongly related mortality treatment success CHF.
ABSTRACT Mutations in the LRP4 gene, coding for a Wnt signaling coreceptor, have been found to cause several allelic conditions. Among these, two are characterized by strong skeletal involvement, namely sclerosteosis and Cenani-Lenz syndrome. In this work, we evaluated role of pathophysiology these diseases. First, report novel mutation, leading substitution arginine at position 1170 glutamine, identified patient with sclerosteosis. This mutation is located central cavity third β-propeller...
Abstract We separated isoenzymes of alkaline phosphatase (ALP; EC 3.1.3.1) in 1383 sera normal individuals (ages 4-65 years) by agarose electrophoresis with the Isopal system (Analis). As expected, predominant isoenzyme children was bone origin, and almost all (99%) had low activities a second fraction, "bone variant" ALP. The disappeared after age 17 girls 20 boys. highest (median) ALP activity reached at 9 to 10 13 14 boys, followed gradual decline steep During adulthood, fraction constant...
A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Bone, liver, high-molecular-mass, and intestinal ALP are separated with high reproducibility. The sensitivity superior to cellulose acetate in detecting high-Mr ALP. Correlation good between bone fractions scanned before after treatment neuraminidase. Immunoglobulin-bound ALPs, ALP-lipoprotein-X complex, additional fraction observed transient hyperphosphatasemia...
To report a case of D-lactic acid acidosis owing to massive oral ingestion propylene glycol.A 72-year old man with known congestive failure was admitted the ICU encephalopathy. Twelve hours prior admission he had erroneously ingested large amount glycol (PG). The laboratory revealed high anion gap (anion = 27 meq/l) (arterial pH 7.16) and an increased osmolal gap. Toxicological analysis low serum level. Biochemical indicated that very amounts (up 110 mmol/l), but not usual type L-lactic...
Autophagy and the unfolded protein response (UPR) are key cellular homeostatic mechanisms both involved in liver diseases, including nonalcoholic fatty disease (NAFLD). Although increasing but conflicting results link these to lipid metabolism, their role potential cross talk herein have been poorly investigated. Therefore, we assessed effects of hepatocyte-specific autophagy deficiency on parenchyma, UPR, metabolism. Adult hepatocellular-specific autophagy-deficient mice ( Atg7 F/F Alb-Cre...
<h3>Background</h3> Resistance to the insulin-sensitising adipocytokine, adiponectin, has been described at level of skeletal muscle in patients with chronic heart failure (CHF). <h3>Objective</h3> To investigate whether exercise training (ET) would improve energy metabolism and adiponectin signalling. <h3>Methods</h3> In a prospective cohort study, CHF were recruited from Cardiac Rehabilitation Centre, Antwerp University Hospital. They underwent 4 months9 combined endurance–resistance ET....
Proposal of a risk analysis model to diminish negative impact on patient care by preanalytical errors in blood gas (BGA).Here we designed Failure Mode and Effects Analysis (FMEA) assessment template for BGA, based literature references expertise an international team laboratory clinical health professionals.The FMEA identifies pre-analytical process steps, that may occur whilst performing BGA (potential failure mode), possible consequences effect) preventive/corrective actions (current...
BACKGROUND: Adynamic bone disease (ABD) has been described in the current dialysis population to have an unexpectedly high prevalence. Moreover, it is clearly more prevalent CAPD patients, compared haemodialysis patients. Recently we demonstrated that both a low (< or = 27 U/1) level of alkaline phosphatase (BAP) as determined by optimized agarose gel electrophoretic technique and 150 pg/ml ) iPTH are good markers ABD with sensitivities 78.1% 80.6% specificities 86.4% 76.2% respectively....
A total of 15-20% type 1 diabetic patients have parietal cell antibodies (PCAs). PCA+ subjects are at increased risk for iron deficiency anemia and atrophic gastritis. Recently, soluble transferrin receptor (sTfR) levels proven to be a sensitive indicator They are, in contrast with ferritin levels, independent inflammation, liver hormonal status, sex. We the first evaluate sTfR diabetes tested hypothesis higher PCAs and/or autoimmune gastritis.We examined 148 (85 men 63 women; 50 were PCA+)...
Apoptosis of macrophages and vascular smooth muscle cells (VSMCs) in advanced atherosclerotic plaques contributes to plaque progression instability. Caspase‐3, a key executioner protease the apoptotic pathway, has been identified human mouse but its role atherogenesis is not fully explored. We therefore investigated impact caspase‐3 deletion on atherosclerosis by crossbreeding knockout (Casp3 −/− ) mice with apolipoprotein E (ApoE mice. Bone marrow‐derived VSMCs isolated from Casp3 ApoE were...
Abstract Objectives This study aimed to evaluate discrepancies in potassium measurements between point-of-care testing (POCT) and central laboratory (CL) methods, focusing on the impact of hemolysis these its clinical practice emergency department (ED). Methods A retrospective analysis was conducted using data from three European university hospitals: Technische Universitat München (Germany), Hospital Universitario La Paz (Spain), Erasmus University Medical Center (The Netherlands). The...
Early treatment of patients with malignant disease and liver or bone metastasis may increase their survival time. We have used the activity patterns isoenzymes alkaline phosphatase (ALP), separated by agarose gel electrophoresis, to detect early metastasis. studied ALP isoenzyme in a background population 101 no evidence any that might influence this pattern; healthy reference (n = 330); following three groups patients: 143 disease, 47 nonmalignant 22 disease. Cutoff predictive values ALP,...