A5103167014- Single-cell and spatial transcriptomics
- Advanced Radiotherapy Techniques
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Immune Cell Function and Interaction
- Advanced X-ray and CT Imaging
- Medical Imaging Techniques and Applications
- Cancer Genomics and Diagnostics
- Radiation Therapy and Dosimetry
- RNA Research and Splicing
- Gene expression and cancer classification
- Autism Spectrum Disorder Research
- Adolescent and Pediatric Healthcare
- Genetic factors in colorectal cancer
- Cardiac, Anesthesia and Surgical Outcomes
- T-cell and B-cell Immunology
- Obsessive-Compulsive Spectrum Disorders
- Genetic Associations and Epidemiology
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Prostate Cancer Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- Cardiac Valve Diseases and Treatments
- Mechanical Circulatory Support Devices
- Endometrial and Cervical Cancer Treatments
City Of Hope National Medical Center
2021-2025
Cork University Hospital
2024
Rutgers, The State University of New Jersey
2024
Newcastle upon Tyne Hospitals NHS Foundation Trust
2018-2023
University of Rochester Medical Center
2021
City of Hope
2021
University of Southern California
2016-2020
Pfizer (United Kingdom)
2020
Southern California University for Professional Studies
2019
USC Norris Comprehensive Cancer Center
2019
283 Background: Males have increased risk and mortality rates of colorectal cancer (CRC) compared to females, but the reasons for these disparities are not well understood. Immune responses differ by sex, a robust T cell response is an important prognostic indicator in CRC. We hypothesized that females exhibit stronger CRC-associated response, contributing reduced males. Methods: The study cohort included 2,751 incident CRC patients (1,337 female; 1,414 male) from population-based...
The incidence of colorectal cancer (CRC) among individuals younger than age 50 (early-onset CRC [EOCRC]) has substantially increased, and yet the etiology molecular mechanisms underlying this alarming rise remain unclear. We compared tumor-associated T-cell repertoires between EOCRC average-onset (AOCRC) to uncover potentially unique immune microenvironment-related features by onset. Our discovery cohort included 242 patients who underwent surgical resection at Cleveland Clinic from 2000...
Recently, measurement of RNA at single cell resolution has yielded surprising insights. Methods for single-cell sequencing (scRNA-seq) have received considerable attention, but the broad reliability methods and factors governing their performance are still poorly known.
To evaluate the contribution of germline genetics to regulating briskness and diversity T cell responses in CRC, we conducted a genome-wide association study examine associations between genetic variation quantitative measures landscapes 2,876 colorectal tumors from participants Molecular Epidemiology Colorectal Cancer Study (MECC).
This paper details a practical method for the direct reconstruction of high dose rate 192Ir source dwell positions in ring applicators using gafchromic film treatment planning brachytherapy cervix patients. It also uncertainties associated with such process. Eight Nucletron interstitial applicators—Ø26 mm (×4), Ø30 (×3) and Ø34 (×1), one 60 intrauterine tube were used this study. RTQA2 XRQA2 films irradiated at pre-programmed three successive sources to derive coordinates positions. The was...
Objective Reduced diversity at Human Leukocyte Antigen (HLA) loci may adversely affect the host’s ability to recognize tumor neoantigens and subsequently increase disease burden. We hypothesized that increased heterozygosity HLA is associated with a reduced risk of developing colorectal cancer (CRC). Methods imputed class I II four-digit alleles using genotype data from population-based study 5,406 cases 4,635 controls Molecular Epidemiology Colorectal Cancer Study (MECC). Heterozygosity...
Stereotactic body radiotherapy for early stage non-small cell lung cancer is an emerging treatment option in the UK. Since relatively few high-dose ablative fractions are delivered to a small target volume, consequences of geometric miss potentially severe. This paper presents results delivery set-up data collected using Elekta Synergy (Elekta, Crawley, UK) cone-beam CT imaging 17 patients immobilised Bodyfix system (Medical Intelligence, Schwabmuenchen, Germany). Images were acquired on...
Abstract Most single cell RNA sequencing protocols start with cells dispersed from intact tissue. High-throughput processing of the separated is enabled using microfluidics platforms. However, dissociation tissue results in loss information about location and morphology potentially alters transcriptome. An alternative approach for collecting to re-purpose electrophysiological technique patch clamp recording. A hollow pipette attached individual cells, enabling recording electrical activity,...
Abstract BACKGROUND GWAS of schizophrenia demonstrated that variations in the non-coding regions are responsible for most common variation heritability disease. It is hypothesized these risk variants alter gene expression. Thus, studying alterations expression may provide a direct approach to understanding etiology In this study we use C ultured N eural progenitor cells derived from O lfactory euroepithelium (CNON) as genetically unaltered cellular model elucidate neurodevelopmental aspects...
Magnetic Resonance (MR)-only prostate radiotherapy using synthetic Computed Tomography (sCT) algorithms with high dose accuracy has been clinically implemented. MR images can suffer from geometric distortions so Quality Assurance (QA) an independent, geometrically accurate, image could be required. The first-fraction Cone Beam CT (CBCT) demonstrated potential but not evaluated in a clinical MR-only pathway. This study the use of CBCT for QA radiotherapy.A total 49 patients treated were...
Objective: To compare the dosimetric consequences of volumetric modulated arc therapy (VMAT) for high-dose palliative thoracic radiotherapy through comparison with conventionally used isocentric parallel opposed pair (POP) fields. Methods: 20 consecutive patients non-small cell lung cancer who received 36 Gy in 12 fractions using a POP technique were re-planned single VMAT arc. Salient parameters compared between plans paired t-test. Results: demonstrated superiority; all PTV dose...
The incidence of colorectal cancer (CRC) among individuals under age 50, or early-onset CRC (EOCRC), has been rising over the past few decades for unclear reasons, and etiology disease remains largely unknown. Known genetic risk factors do not explain this increase, pointing to possible environmental as-yet unidentified contributors their interactions. Previous research linked variation on chromosome 6 increased risk. This region harbors multiple immune genes, including gene encoding Major...
Copyright © 2018 Inderscience Enterprises Ltd. Background: Low-input or single-cell RNA-Seq are widely used today, but two technical questions remain: 1) in replicates, what proportion of noises comes from input RNA quantity rather than variation bioinformatics tools?; 2) In single neurons, whether gene expression is attributable to biological heterogeneity just random noise? To examine the sources variability, we have generated data low-input (10/100/1000pg) reference samples and 38 neurons...
This study describes treatment patterns, healthcare resource utilization (HCRU), and costs associated with persistence, switching, dosing of branded celecoxib in osteoarthritis (OA) patients.This retrospective claims database analysis used MarketScan® Commercial Claims Encounters (MarketScan) data from 2009 to 2013. Included patients were adult (≥ 18 years), incident users ≥ 1 OA claim. The switch analyzed outcomes persistent on versus switched a generic nonsteroidal anti-inflammatory drug...
Radiotherapy treatment planning relies upon density information provided by CT for accurate dose calculations. Hounsfield units (HUs) are converted to electron/physical via an energy dependant calibration curve. Multiple curves required make full use of the available accelerating potentials (kVp). The bi-linear with a discontinuity occurring at soft-tissue densities. commercial algorithm, DirectDensityTM (Siemens Healthcare GmbH), constructs single curve covering all kVp. This enables...
Background: Low-input or single-cell RNA-Seq are widely used today, but two technical questions remain: 1) in replicates, what proportion of noises comes from input RNA quantity rather than variation bioinformatics tools?; 2) In single neurons, whether gene expression is attributable to biological heterogeneity just random noise? To examine the sources variability, we have generated data low-input (10/100/1000pg) reference samples and 38 neurons human brains. Results: For negatively...