A5101910195- Enzyme Production and Characterization
- Economic theories and models
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Economic Theory and Policy
- Natural product bioactivities and synthesis
- Catalytic C–H Functionalization Methods
- Banking stability, regulation, efficiency
- Bacterial Genetics and Biotechnology
- Monetary Policy and Economic Impact
- Biofuel production and bioconversion
- Metal-Catalyzed Oxygenation Mechanisms
- Epigenetics and DNA Methylation
- Electrochemical sensors and biosensors
- Genomics, phytochemicals, and oxidative stress
- Genomics and Chromatin Dynamics
- Enzyme-mediated dye degradation
- Advanced Radiotherapy Techniques
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Phytase and its Applications
- Microbial Metabolites in Food Biotechnology
- Economic Policies and Impacts
- Cancer-related gene regulation
- Synthesis and Catalytic Reactions
Kobe University
2013-2024
Anhui University
2015-2024
Huazhong Agricultural University
2024
International Monetary Fund
2021-2023
East China Normal University
2018-2021
Luoyang Normal University
2014-2015
Hebei University
2014
Renmin University of China
2014
University of Science and Technology of China
2013
RMIT University
2007-2009
A crystal structure of an autophagy receptor with a ligand reveals how Salmonella is specifically targeted for degradation.
Herein, we discovered an efficient flavone 6-C-glycosyltransferase GcCGT from the medicinal plant Gentiana crassicaulis. could catalyze consecutive two-step 6-C/4′-O-glycosylation of flavonoids. Homology modeling and site-directed mutagenesis yielded mutant F387K, which unprecedented 6-C-glycosylation 8-C-glycosides to produce 6,8-di-C-glycosides. To elucidate catalytic mechanisms, crystal structures GcCGT-apo (2.10 Å) GcCGT/UDP (2.40 were resolved. Structural analysis molecular dynamics...
Glycosylation is an important post-modification reaction in plant secondary metabolism, and contributes to structural diversity of bioactive natural products. In plants, glycosylation usually catalyzed by UDP-glycosyltransferases. Flavonoid 2'-O-glycosides are rare glycosides. However, no UGTs have been reported, thus far, specifically catalyze 2'-O-glycosylation flavonoids. this work, UGT71AP2 was identified from the medicinal Scutellaria baicalensis as first flavonoid...
Degradation via enzymatic processes for the production of valuable β-1,3-glucooligosaccharides (GOS) from curdlan has attracted considerable interest. CBM6E functions as a curdlan-specific β-1,3-endoglucanase, composed glycoside hydrolase family 128 (GH128) module and carbohydrate-binding (CBM) derived CBM6.
Catalytic selective hydroxylation of unactivated aliphatic (sp3 ) C-H bonds without a directing group represents formidable task for synthetic chemists. Through directed evolution P450BSβ hydroxylase, we realize oxyfunctionalization in broad spectrum carboxylic acids with varied chain lengths, functional groups and (hetero-)aromatic moieties highly chemo-, regio- enantioselective fashion (>30 examples, Cβ/Cα>20 : 1, >99 % ee). The X-ray structure the evolved variant, -L78I/Q85H/G290I,...
Posttranslational rhamnosylation of EF-P plays a key role in Pseudomonas aeruginosa , establishing virulence and antibiotic resistance, as well survival. The detailed structural biochemical characterization the EF-P-specific rhamnosyltransferase EarP from P. not only demonstrates that sugar donor TDP-Rha binding enhances acceptor to but also should provide valuable information for structure-guided development its inhibitors against infections other EarP-containing pathogens.
Abstract Subfamily 37 of the glycoside hydrolase family GH13 was recently established on basis discovery a novel α-amylase, designated AmyP, from marine metagenomic library. AmyP exhibits raw-starch-degrading activity and consists an N-terminal catalytic domain C-terminal starch-binding domain. To understand this newest subfamily, we determined crystal structure named ΔSBD , complexed with maltose, E221Q mutant maltotriose. Glu221 is one three conserved residues, inactivated by mutation....
Saccharomyces cerevisiae Spp1, a plant homeodomain (PHD) finger containing protein, is critical subunit of the histone H3K4 methyltransferase complex proteins associated with Set1 (COMPASS). The chromatin binding affinity PHD Spp1 has been proposed to modulate COMPASS activity. During meiosis, plays another role in promoting programmed double-strand break (DSB) formation by H3K4me3 via its and interacting DSB Mer2. However, how performs site-specific readout still not fully understood. In...
Chalcones are widely present in most plants and have various health beneficial functions. This study investigates the suppressive effect of 13 natural synthetic chalcones on transformation aryl hydrocarbon receptor (AhR) induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) 3-methylcholanthrene (3-MC) a cell-free system, Hepa-1c1c7 cells, liver ICR mice.
Microalgae starch is receiving increasing attention as a renewable feedstock for biofuel production. Raw microalgae from Tetraselmis subcordiformis was proven to be very efficiently hydrolyzed by an α-amylase (AmyP) of glycoside hydrolase subfamily GH13_37 below the temperature gelatinization (40 °C). The hydrolysis degree reached 74.4 ± 2.2% 4% raw and 53.2 1.7% 8% after only 2 h. efficiency significantly stimulated calcium ions. enzyme catalysis AmyP its mutants (Q306A E347A) suggested...
Abstract Background Dye-decolorizing peroxidases (DyPs) represent a novel family of heme that use H 2 O as the final electron acceptor to catalyze oxidation various organic compounds. A DyP from Irpex lacteus F17 ( Il -DyP4, corresponding GenBank MG209114), obtained by heterologous expression, exhibits high catalytic efficiency for phenolic compounds and strong decolorizing ability toward synthetic dyes. However, enzyme structure residues involved in substrate remain poorly understood....