A5085425476- Enzyme-mediated dye degradation
- Meat and Animal Product Quality
- Scientific Computing and Data Management
- Microbial Metabolism and Applications
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- Plant Pathogens and Fungal Diseases
- Fermentation and Sensory Analysis
- Aquaculture Nutrition and Growth
- bioluminescence and chemiluminescence research
- Genetics, Bioinformatics, and Biomedical Research
- Genomics and Phylogenetic Studies
- Electrochemical sensors and biosensors
- melanin and skin pigmentation
- Electrochemical Analysis and Applications
- Fungal Biology and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial Natural Products and Biosynthesis
- Slime Mold and Myxomycetes Research
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Carbohydrate Chemistry and Synthesis
- Microbial Inactivation Methods
- Circular RNAs in diseases
Anhui University
2016-2024
Zhengzhou University of Light Industry
2006-2024
Xi'an Jiaotong University
2023
Hebei Agricultural University
2023
China XD Group (China)
2018
University of Science and Technology of China
2013-2015
University of California, San Diego
2013-2014
University of Montana
2013
Jiangnan University
2008
How easy is it to reproduce the results found in a typical computational biology paper? Either through experience or intuition reader will already know that answer with difficulty not at all. In this paper we attempt quantify by reproducing previously published for different classes of users (ranging from little expertise domain experts) and suggest ways which situation might be improved. Quantification achieved estimating time required each steps method described original make them part an...
Engineering of fungal laccases with optimum catalytic activity at alkaline pH has been a long-lasting challenge. In this study, mutant library containing 3000 clones was obtained by error-prone PCR to adapt the laccase Lcc9 from basidiomycete Coprinopsis cinerea. After three rounds functional screening, amino acid changes (E116K, N229D, I393T) named PIE5 selected. showed an 8.5 and 8.0 against guaiacol 2,6-DMP when expressed in Pichia pastoris, representing first that possesses condition....
Abstract Irpex lacteus , a cosmopolitan white-rot fungus, degrades lignin and lignin-derived aromatic compounds. In this study, we report the high-quality draft genome sequence of I. F17, isolated from decaying hardwood tree in vicinity Hefei, China. The is 44,362,654 bp, with GC content 49.64% total 10,391 predicted protein-coding genes. addition, 18 snRNA, 842 tRNA, 15 rRNA operons 11,710 repetitive sequences were also identified. genomic data provides insights into mechanisms efficient...
Numerous studies have reported that microRNA (miR)-216b, as a tumor suppressor, is downregulated in variety of cancer types. PDZ binding kinase (PBK)/T-LAK-cell-originated protein (TOPK) highly expressed various types human cancer, including lung cancer. The expression miR-216b-3p and its potential roles adenocarcinoma are still unclear no research has been conducted into the association between PBK/TOPK. Thus, present study aimed to investigate role explore whether PBK/TOPK involved...
Maillard reaction products (MRPs) play a crucial role in preserving food quality due to their antioxidant capacity. In this study, we isolated and characterized the components from MRPs using xylose-lysine system under optimal conditions. Methylene chloride ethyl acetate were employed for sequential extractions, phase underwent thin-layer chromatography silica column separation. The identified compound, 3-hydroxy-4-methyl-2(5 H)-furanone (3H4MFN), was analyzed FT-IR, GC-MS, NMR, revealing...
We here use an approach of active site alignment and clustering many evolutionarily distant enzymes catalyzing alike reactions to identify conserved residues/interactions that may play key chemical roles in catalysis. Then density functional theory (DFT) calculations on cluster models are used investigate the essentialness such its mechanistic basis. apply this 130 glycoside hydrolases (GHs) (βα)8-barrel fold. These adopt either a classical retaining mechanism or substrate-assisted...
Abstract Background Dye-decolorizing peroxidases (DyPs) represent a novel family of heme that use H 2 O as the final electron acceptor to catalyze oxidation various organic compounds. A DyP from Irpex lacteus F17 ( Il -DyP4, corresponding GenBank MG209114), obtained by heterologous expression, exhibits high catalytic efficiency for phenolic compounds and strong decolorizing ability toward synthetic dyes. However, enzyme structure residues involved in substrate remain poorly understood....
Summary A novel, simple and reliable spectrophotometric method to determine sodium chloride in salted fish was developed validated. The based on the reaction of with silver nitrate determination turbidity formed. absorbance measured at 385 nm. tested under various conditions, we confirmed an optimal operation 2 mL 1:4 (v/v) nitric acid, 1.5 g L −1 gelatin, 5 0.5% a total volume 50 mL, mixed heated 60 °C for 10 min. range linearity detection limit were 0.4–24 mg 0.2 , respectively. relative...
ABSTRACT Malaria is a disease contracted by over 200 million people each year, mostly in developing countries. The primary causative agent, Plasmodium falciparum ( P. ) has shown increased resistance to existing drugs, hence new treatments are needed quickly. To this end we performed high-throughput systems-level analysis, mapping FDA drugs with the potential for repurposing against targets from structural proteome. resulting drugome P.falciparum -drugome) was used prioritize anti-malaria...
Laccase is a widely-used environment-friendly copper-containing oxidase found in many plants, insects and fungi. Recently, more laccases are also bacteria. Myxobacteria an important bacteria resource. However, myxobacteria much difficult to isolate purify than other We used bioinformatic approach screen proteomes available NCBI. Based on conserved sequences of four copper binding sites multicopper oxidase, 30 potential laccase were obtained. Among them, nine genes synthesized expressed...
The aim of this study was to clone the chicken zp1 gene encoding zona pellucida 1 (Zp1) and investigate its tissues expression profile effect on osteoblast mineralization. level quantified in various laying hens tibia pre- post-sexual maturity by RT-qPCR. Zp1 overexpressed vector transfected into calvarial osteoblasts which were induced differentiation for 8 days, extracellular mineral mineralization-related genes detected. full-length is 3 045 bp, 958 amino acids residuals, has two...