Abstract Background Continual expression of PD-L1 in tumor cells is critical for immune escape and host T cell exhaustion, however, knowledge on its clinical benefits through inhibition limited breast cancer. N 6 -methyladenosine (m A) plays a crucial role multiple biological activities. Our study aimed to investigate the regulatory m A modification surveillance Methods MeRIP-seq epitranscriptomic microarray identified that downstream target METTL3. MeRIP-qPCR, absolute quantification assay,...

Abstract Background Emerging evidence suggests that epithelial mesenchymal transition (EMT) and epigenetic mechanisms promote metastasis . Histone deacetylases (HDACs) noncoding RNAs (ncRNAs) are important regulators. Here, we elucidated a novel role of histone deacetylase 2 ( HDAC2 ) in regulating EMT CRC via ncRNA. Methods The expression HDACs was analyzed using the public databases matched primary metastatic tissues, cells with different potentials (DLD1, HCT116, SW480 SW620). Microarray...

Objectives To clarify the key role of circulating interferon-γ (IFN-γ) and to improve clinical efficacy mesenchymal stem cell (MSC) transplantation (MSCT) in patients with rheumatoid arthritis (RA). Methods Study wild-type or IFN-γR -/- MSCT was first evaluated a murine model collagen-induced (CIA) following which phase 1/2 randomised controlled study conducted 63 RA who responded poorly regular treatments. Subjects were randomly assigned an monotherapy group (n=32) plus recombinant human...

Irpex lacteus F17 is well-known for its ability to degrade recalcitrant aromatic pollutants, which mainly results from the action of manganese peroxidase (MnP) that it able produce. Recently, genome sequencing and annotation this strain provided comprehensive picture ligninolytic gene family. In addition revealing presence 13 MnPs, genes five dye-decolorizing peroxidases (DyPs) were also discovered in I. genome, are unrelated fungal class II peroxidases. present study, amino acid sequences...

Chronic non-healing wounds fail to progress beyond the inflammatory phase, characterized by a disorder of inflammation resolution.PD-1/PD-L1, major co-inhibitory checkpoint signaling, plays critical roles in tumor immune surveillance and occurrence or autoimmune diseases, but its wound healing remains unclear.Here, we described novel function PD-L1 fibroblast-like cells as positive regulator healing.PD-L1 dynamically expressed on granulation tissue during form immunosuppressive...

The immunosuppressive function “licensed” by IFN-γ is a vital attribute of mesenchymal stem cells (MSCs) widely used in the treatment inflammatory diseases. However, mechanism and impact metabolic reprogramming on MSC immunomodulatory plasticity remain unclear. Here, we explored which glucose metabolism affects MSCs IFN-γ. Our data showed that regulates human umbilical cord (hUC-MSCs) challenged through Janus kinase–signal transducer activator transcription (JAK-STAT) pathway. Furthermore,...

The immunosuppressive capacity of mesenchymal stem cells (MSCs) is dependent on the "license" several proinflammatory factors to express such as programmed cell death 1 ligand (PD-L1), which determines clinical therapeutic efficacy MSCs for inflammatory or immune diseases. In MSCs, interferon-gamma (IFN-γ) a key inducer PD-L1 expression, synergistically enhanced by tumor necrosis factor-alpha (TNF-α); however, underlying mechanism unclear.To reveal pretreated high and explore application in...

Cardiovascular complications, especially myocardial infarctions (MIs), are the leading mortality cause in diabetic patients. The transplantation of stem cells into damaged hearts has had considerable success as a treatment for MI, although whether antidiabetic drugs affect therapeutic efficacy cell is still unknown. This study aims to understand and how metformin, one first-line used treat type 2 diabetes mellitus (T2DM), induces mesenchymal stromal (MSC) apoptosis dampens their...

Abstract Irpex lacteus , a cosmopolitan white-rot fungus, degrades lignin and lignin-derived aromatic compounds. In this study, we report the high-quality draft genome sequence of I. F17, isolated from decaying hardwood tree in vicinity Hefei, China. The is 44,362,654 bp, with GC content 49.64% total 10,391 predicted protein-coding genes. addition, 18 snRNA, 842 tRNA, 15 rRNA operons 11,710 repetitive sequences were also identified. genomic data provides insights into mechanisms efficient...

To elucidate the underlying mechanism by which proliferation and migration abilities of human umbilical cord mesenchymal stem cells (hUC-MSCs) determine their therapeutic efficacy in rheumatoid arthritis treatment.

Rationale: Desmoplastic small round cell tumor (DSRCT) is a rare, aggressive and malignant tumor. This report describes case involving DSRCT of the middle ear which no has been reported in literature till date. Patient concern: A 59-year-old Chinese man with 40-year history repeated suppuration his right 1-year drooping angle mouth. The CT brain scan enhanced MRI showed space occupying lesion ear. Diagnoses: Interventions: After relevant examinations, radical mastoidectomy subtotal temporal...

Background: High altitude pulmonary edema (HAPE) is a serious mountain sickness with certain mortality. Its early diagnosis very important. However, the mechanism of its onset and progression still controversial. Aim: This study aimed to analyze HAPE occurrence development search for prospective biomarkers in peripheral blood. Methods: The difference genes (DEGs) Control group were enriched by gene ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis, then GSEA analysis...

This case-control study investigates the possible relationships between single-nucleotide polymorphisms rs1052133 in human 8-oxoguanine DNA glycosylase 1 (hOGG1) gene and rs3219472 MutY homologue (hMUTYH) risk of nasopharyngeal carcinoma (NPC). The two were genotyped 488 unrelated NPC patients 573 cancer-free controls. Genotype GG at was associated with significantly lower than genotypes GC + CC (odds ratio [OR] 0.770, 95% confidence interval [CI] 0.595-0.996, P=0.012). In subgroup analyses,...

Micro- and macro-vascular events are directly associated with hyperglycemia in patients type 2 diabetes mellitus (T2DM), but whether intensive glucose control decreases the risk of diabetic cardiovascular complications remains uncertain. Many studies have confirmed that impaired quality quantity mesenchymal stem cells (MSCs) plays a pathogenic role diabetes. Our previous study found abundance circulating MSCs was significantly decreased T2DM, which correlated progression complications. In...

Allergic rhinitis (AR) is a common disease that causes severe inflammation and even disabilities. Previous studies have reported baicalein to an anti-inflammatory effect. However, the pharmacological action of on anaphylaxis has not been clarified yet. This study assessed in vivo protective effect post-treatment ameliorating ovalbumin (OVA)-sensitized AR rat model. Baicalein attenuated histological alterations, aberrant tissue repair after OVA-induced AR. reduced frequency nasal/ear rubs...

Abstract Rationale: Cartilaginous tumors of the larynx are rare. This report describes an atypical case with chondroma laryngeal cartilage presenting as cervical mass, which was misdiagnosed a thyroid tumor. Patient concern: A 73-year-old Chinese man 1-month history mass. The neck color Doppler ultrasound and CT showed space-occupying lesion in upper right pole gland. Diagnoses: Chondroma confirmed at time surgery. Interventions: After relevant examinations, subtotal thyroidectomy excisional...

Background Apurinic/apyrimidinic endonuclease 1 ( APEX 1) plays a central role in the repair of oxidative DNA lesions via base excision repair, and polymorphism gene may affect susceptibility to carcinogenesis. Methods Here, we assessed possible relationships between single‐nucleotide at rs1760944 risk nasopharyngeal carcinoma NPC ) 477 patients 558 healthy controls from Guangxi province, which is second largest endemic area South China. Results Genotype frequencies were Hardy‐Weinberg...

The aim of this study was to explore potential relationships four single-nucleotide polymorphisms (SNPs) in the gene encoding dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) with risk nasopharyngeal carcinoma (NPC). DC-SIGN SNPs rs7252229, rs4804803, rs2287886, and rs735240 were genotyped 477 unrelated NPC patients 561 cancer-free controls. At significantly lower individuals GC (odds ratio [OR] 0.076, 95% confidence interval [CI] 0.008–0.690), GG (OR...

Programmed cell death 1 ligand (PD-L1) is an important immunosuppressive molecule, which inhibits the function of T cells and other immune by binding to receptor programmed death-1. The PD-L1 expression disorder plays role in occurrence, development, treatment sepsis or inflammatory diseases, has become target for these diseases. Mesenchymal stem (MSCs) are a kind pluripotent with multiple differentiation potential. In recent years, MSCs have been found strong ability used treat various...

Abstract Background: Emerging evidence suggests that epithelial mesenchymal transition (EMT) and epigenetic mechanisms promote metastasis . Histone deacetylases (HDACs) noncoding RNAs (ncRNAs) are important regulators. Here, we elucidated a novel role of histone deacetylase 2 ( HDAC2 ) in regulating EMT CRC via ncRNA. Methods: The expression HDACs was analyzed using the public databases matched primary metastatic tissues, cells with different potentials (DLD1, HCT116, SW480 SW620)....

To evaluate the potential association of variations in number tandem repeats dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) and nonintegrin-related (DC-SIGNR) neck region with susceptibility to nasopharyngeal carcinoma (NPC).Variations genotypes alleles DC-SIGN/DC-SIGNR were analyzed 477 unrelated NPC patients 561 cancer-free controls.Genotypes DC-SIGN did not differ significantly between controls, but 9-repeat genotype DC-SIGNR was more frequent...