A5100461202- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cervical Cancer and HPV Research
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- Sulfur Compounds in Biology
- Lung Cancer Treatments and Mutations
- Advanced Breast Cancer Therapies
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Acute Ischemic Stroke Management
- RNA modifications and cancer
- Inflammasome and immune disorders
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Cerebrovascular and Carotid Artery Diseases
- Lipid metabolism and biosynthesis
- Natural product bioactivities and synthesis
- Hepatitis B Virus Studies
- RNA Research and Splicing
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Ubiquitin and proteasome pathways
- Liver physiology and pathology
- Cancer Research and Treatments
Nanjing Medical University
2008-2025
Jiangsu Province Hospital
2008-2025
Oslo University Hospital
2015-2024
University of Oslo
2015-2024
Dalian Medical University
2023-2024
Chongqing Medical University
2023-2024
Second Affiliated Hospital of Chongqing Medical University
2023-2024
Wuxi Ninth People's Hospital
2024
Ningxia Medical University
2024
Soochow University
2024
Abstract Purpose: mTOR (mammalian target of rapamycin) plays a central role in regulating cell growth and cycle progression is regarded as promising therapeutic target. We examined whether inhibition by RAD001 (everolimus) therapeutically efficacious the treatment ovarian cancer single agent combination with cisplatin. Experimental Design: Using four human lines, we determined effect 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Western blot, apoptosis assays. evaluated...
The peroxiredoxin (PRX) family of antioxidant enzymes helps maintain the intracellular reducing milieu and suppresses apoptosis in non-neuronal cells. However, whether PRX can inhibit neuronal through specific signaling mechanisms remains poorly understood. Induction PRX2, most abundant PRX, occurs Parkinson's disease (PD) patient brains, but its functional impact is unclear. In present study, we used dopaminergic (DA) toxin 6-hydroxydopamine (6-OHDA) to model PD explore protective effect on...
The damage borne by the endothelial cells (ECs) forming blood-brain barrier (BBB) during ischemic stroke and other neurological conditions disrupts structure function of neurovascular unit contributes to poor patient outcomes. We recently reported that structural aberrations in brain microvascular ECs-namely, uncontrolled actin polymerization subsequent disassembly junctional proteins, are a possible cause early onset BBB breach arises within 30-60 min reperfusion after transient focal...
Pulmonary hypertension is a serious condition that can lead to premature death. The mechanisms involved are incompletely understood although role for the immune system has been suggested. Inflammasomes part of innate and consist effector caspase-1 receptor, where nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) best characterized interacts with adaptor protein apoptosis-associated speck-like containing caspase-recruitment domain (ASC). To investigate...
Peroxiredoxins (PRXs) are a newly characterized family of peroxide scavenging enzymes that not only help maintain cellular redox homeostasis but also may directly engage in variety intracellular signaling pathways. PRX2 is neuronal-specific PRX believed to participate cerebral antioxidant responses several neurodegenerative diseases. This study investigates the potential neuroprotective effect and underlying mechanism models ischemic neuronal injury.Transgenic mice overexpressing showed...
Merocyanines adsorbed into the mesopores of mSiO2 shell NaYF4: 20% Yb, 2% Er, 0.2% Tm nanocrystals are demonstrated as ratiometric upconversion luminescence (UCL) probe for highly selective detection HS(-) in living cells through inhibition energy transfer from UCL to absorbance merocyanines. The has been used sensing H2S with high sensitivity and selectivity.
The human CDKN2A locus encodes 2 distinct proteins, p16(INK4A) and p14(ARF) [mouse p19(Arf)], designated INK4A (inhibitor of cyclin dependent kinase 4) ARF (alternative reading frame) here, that are translated from alternatively spliced mRNAs. Human is implicated as a tumor suppressor gene, mainly in association with the simultaneous deletion . However, questions remain to whether loss alone sufficient drive tumorigenesis. Here, we report mice deficient for Arf susceptible accelerated...
The CDKN2A/ARF locus encompasses overlapping tumor suppressor genes p16(INK4A) and p14(ARF), which are frequently co-deleted in human malignant mesothelioma (MM). importance of loss cancer is well established, but the relative significance p14(ARF) has been debated. predisposition mice singly deficient for either Ink4a or Arf, due to targeting exons 1α 1β, respectively, supports idea that both play significant nonredundant roles suppressing spontaneous tumors. To further test this notion, we...
Bacteria of the Burkholderia cepacia complex (Bcc) can cause devastating pulmonary infections in cystic fibrosis (CF) patients, yet precise mechanisms underlying inflammation, recurrent exacerbations and transition from chronic stages to acute infection septicemia are not known. Bcc bacteria generally believed have a predominant extracellular biofilm life style infected CF lungs, similar Pseudomonas aeruginosa, but this has been challenged by clinical observations which show predominantly...
Generation of reproducible cardiac disease phenotypes in mice is instrumental for investigating mechanisms leading to heart failure (HF). For decades, suture-based thoracic aortic constriction has been the preferred method increasing left ventricular (LV) afterload rodents, but degree stenosis resulting from this variable. In an effort improve methodology, we subjected ascending aorta using o-rings with fixed inner diameters (IDs).Mice C57BL/6J and FVB/N background were IDs 0.71, 0.66, 0.61...
Endometriosis (Ems) is a condition that refers to the ectopic implantation and growth of endometrial tissue outside uterine cavity. The aim present study was investigate role microRNA‑138 (miR‑138) in Ems possible underlying mechanism. Flow cytometry measured CD11b level, cell proliferation using MTT assay lactate dehydrogenase (LDH) assays analyzed LDH activity kits. Cell apoptosis Annexin V‑FITC/PI double staining detection kit DAPI assays. ELISA western blot analysis were used measure...
Significance Therapies that boost brain plasticity and repair processes without deleterious side effects have the potential to transform clinical management of stroke. Here we report tissue plasminogen activator (tPA), known as a clot buster for treatment ischemic stroke, exerts additional neurorestorative in murine model stroke by stimulating axonal regeneration facilitating functional recovery. Importantly, restoration was independent tPA’s proteolytic activity—a mutant, protease-inactive...
Understanding the time-dependent nonlinear stress–strain response of carbon fiber reinforced polymer composites (CFRPs) is critical for reliable performance in lightweight designs such as aircrafts and transport vehicles. However, accurately predicting this rate-dependent behavior remains challenging due to significant hardening effects thermoplastic matrix stress-strain responses thermoset composites. In work, we develop a new micromechanics-based constitutive model using method cells...
Ceramides are known for their harmful, cell-autonomous effects in cigarette smoke (CS)-triggered chronic obstructive pulmonary disease (COPD), yet potential role as intercellular signals COPD pathogenesis remains unclear. This study aims to investigate whether ceramides act cell-nonautonomous mediators of development by transmitting metabolic stress from macrophages endothelial cells (ECs), compromising function and thereby orchestrating the inflammation. We analyzed single-cell RNA...
We previously demonstrated that ginsenosides Rg1 and Re enhanced the immune response in C3H/HeB mice but not C3H/HeJ carrying a mutation Tlr4 gene. The results of present study showed both inhibited mRNA expression production proinflammatory mediators included tumor necrosis factor α, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase from lipopolysaccharide (LPS)-stimulated macrophages. was found to be distributed extracellularly intracellularly located only...
Hydrogen sulfide (H 2 S), a novel endogenous gasotransmitter, has been considered neuromodulator to enhance hippocampal long‐term potentiation and exerts neuroprotective effects against neurotoxin‐induced neurodegeneration in rodent models of Parkinson's disease (PD). However, whether H S can function as regulate the levels nigrostriatal neurotransmitters then impact vulnerability dopaminergic (DA) neurons response neurotoxins remains unknown. For this study, we prepared...
Abstract Objectives To investigate the synergistic mechanisms of Paris Saponin II ( PSII ) and Curcumin CUR in lung cancer. Materials Methods The combination changed cellular uptake , apoptosis, cell cycle arrest cytokine levels were analysed on different cancer cells. Results displayed a anti‐cancer effect through promoting Hoechst H33258 staining FACS assay indicated that induced apoptosis. Western blot antibody microarray suggested activated death receptors such as DR 6, CD 40/ 40L, FasL...