A5017218126- Esophageal Cancer Research and Treatment
- Gastric Cancer Management and Outcomes
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Esophageal and GI Pathology
- Cancer Research and Treatments
- Colorectal Cancer Screening and Detection
- Lung Cancer Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Lung Cancer Treatments and Mutations
- Radiomics and Machine Learning in Medical Imaging
- Angiogenesis and VEGF in Cancer
- Gastrointestinal Tumor Research and Treatment
- Photoacoustic and Ultrasonic Imaging
- Colorectal Cancer Surgical Treatments
- Metastasis and carcinoma case studies
- Radiopharmaceutical Chemistry and Applications
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Monoclonal and Polyclonal Antibodies Research
- Chemical Reactions and Isotopes
- Inflammatory Bowel Disease
- Colorectal Cancer Treatments and Studies
- Microscopic Colitis
- Optical Imaging and Spectroscopy Techniques
University Medical Center Groningen
2016-2025
University of Groningen
2016-2025
Radboud University Nijmegen
2023
Radboud University Medical Center
2023
RELX Group (Netherlands)
2022
Dialyse Centrum Groningen
2020
Hanze University of Applied Sciences
2013
Ghent University Hospital
2013
Washington University in St. Louis
2004
Vascular endothelial growth factor (VEGF), released by tumor cells, is an important in angiogenesis. The humanized monoclonal antibody bevacizumab blocks VEGF-induced angiogenesis binding, thereby neutralizing VEGF. Our aim was to develop radiolabeled for noninvasive vivo VEGF visualization and quantification with the single gamma-emitting isotope 111In PET 89Zr.Labeling, stability, binding studies were performed. Nude mice a human SKOV-3 ovarian xenograft injected 89Zr-bevacizumab,...
Background: Heart failure (HF) survival has improved, and nowadays, many patients with HF die of noncardiac causes, including cancer. Our aim was to investigate whether a causal relationship exists between the development Methods: induced by inflicting large anterior myocardial infarction in APC min mice, which are prone developing precancerous intestinal tumors, tumor growth measured. In addition, rule out hemodynamic impairment, heterotopic heart transplantation model used an infarcted or...
Purpose: To provide proof of principle safety, breast tumor-specific uptake, and positive tumor margin assessment the systemically administered near-infrared fluorescent tracer bevacizumab-IRDye800CW targeting VEGF-A in patients with cancer.Experimental Design: Twenty primary invasive cancer eligible for surgery received 4.5 mg as intravenous bolus injection. Safety aspects were assessed well uptake delineation during ex vivo surgical specimens using an optical imaging system. Ex multiplexed...
Fluorescence imaging is currently attracting much interest as a method for intraoperative tumor detection, but most current tracers lack specificity. Therefore, this technique can be further improved by tumor-specific detection. With tumor-targeted antibodies bound to radioactive label, SPECT or PET feasible in the clinical setting. The aim of present study was apply antibody-based detection optical imaging, using preclinical vivo mouse models. <b>Methods:</b> Anti–vascular endothelial...
Several in vitro and vivo models have revealed the key role of CXCR4/CXCL12 axis tumor-stroma interactions. Stromal cells present tumor microenvironment express high levels CXCL12 protein, directly stimulating proliferation migration CXCR4-expressing cancer cells. This specific prosurvival influence stromal on is thought to protect them from cytotoxic chemotherapy postulated as a possible explanation for minimal residual disease hematological solid cancers. Therefore, signaling an attractive...
Abstract During the last decade, emerging field of molecular fluorescence imaging has led to development tumor-specific fluorescent tracers and an increase in early-phase clinical trials without having consensus on a standard methodology for evaluating optical tracer. By combining multiple complementary state-of-the-art techniques, we propose novel analytical framework translation evaluation tumor-targeted which can be used range tumor types with different tracers. Here report implementation...
No validated predictive biomarkers for antiangiogenic treatment of metastatic renal cell carcinoma (mRCC) exist. Tumor vascular endothelial growth factor A (VEGF-A) level may be useful. We determined tumor uptake <sup>89</sup>Zr-bevacizumab, a VEGF-A–binding PET tracer, in mRCC patients before and during pilot study. <b>Methods:</b> Patients underwent <sup>89</sup>Zr-bevacizumab scans at baseline 2 6 wk after initiating either bevacizumab (10 mg/kg every wk) with interferon-α (3–9 million IU...
The incidence of oesophageal adenocarcinoma (EAC) is steeply rising. Early lesion detection a critical factor for improving disease prognosis. We developed and investigated wide-field near-infrared fluorescence molecular endoscopy (NIR-FME), using systemic topical administration fluorescence-labelled antibody against vascular endothelial growth (VEGFA). Fourteen patients with Barrett’s oesophagus (BE) underwent endoscopic mucosal resection (EMR) combined with NIR-MFE. From total 20 confirmed...
Objective Radiofrequency ablation (RFA)±endoscopic resection (ER) is the preferred treatment for early neoplasia in Barrett’s oesophagus (BE). We aimed to report short-term and long-term outcomes all 1384 patients treated Netherlands (NL) from 2008 2018, with uniform follow-up (FU) a centralised setting. Design Endoscopic therapy BE NL nine expert centres specifically trained endoscopists pathologists that adhere joint protocol. Prospectively collected data are registered database. Patients...
Tumor-positive resection margins are present in up to 23% of head and neck cancer (HNC) surgeries, as intraoperative techniques for real-time evaluation the lacking.In this study, we investigated safety potential clinical value fluorescence-guided imaging (FGI) margin HNC patients.We determined optimal cetuximab-800CW dose by quantification intrinsic fluorescence values using multi-diameter single-fiber reflectance, (MDSFR/SFF) spectroscopy.Methods: Five cohorts three patients received...
Inadequate surgical margins occur frequently in oral squamous cell carcinoma surgery. Fluorescence molecular imaging (FMI) has been explored for intraoperative margin assessment, but data are limited to phase-I studies. In this single-arm phase-II study (NCT03134846), our primary endpoints were determine the sensitivity, specificity and positive predictive value of cetuximab-800CW tumor-positive detection. Secondary safety, close detection rate intrinsic fluorescence. 65 patients with 66...
Abstract Introduction Endoscopic detection of early neoplasia in Barrett's esophagus is difficult. Computer Aided Detection (CADe) systems may assist detection. The aim this study was to report the first steps development a CADe system for and evaluate its performance when compared with endoscopists. Methods This developed by consortium, consisting Amsterdam University Medical Center, Eindhoven Technology, 15 international hospitals. After pretraining, trained validated using 1.713...
BackgroundComputer-aided detection (CADe) systems could assist endoscopists in detecting early neoplasia Barrett's oesophagus, which be difficult to detect endoscopic images. The aim of this study was develop, test, and benchmark a CADe system for oesophagus.MethodsThe first pretrained with ImageNet followed by domain-specific pretraining GastroNet. We trained the on dataset 14 046 images (2506 patients) confirmed oesophagus non-dysplastic from 15 centres. Neoplasia delineated experts all...
Abstract In the K/B×N mouse model of arthritis, autoantibodies against glucose-6-phosphate isomerase cause joint-specific inflammation and destruction. We have shown using micro-positron emission tomography that these isomerase-specific rapidly localize to distal joints mice. this study we used delineate stages involved in development arthritis. Localization Abs depended upon mast cells, neutrophils, FcRs, but not on C5. Surprisingly, anti-type II collagen alone did accumulate joints, could...
Angiogenesis is a critical step in tumor development, which vascular endothelial growth factor (VEGF) key aspect. Heat shock protein 90 (HSP90), molecular chaperone, essential for the activity of proteins involved VEGF transcription. Currently, no biomarkers to predict effect of, or monitor, HSP90 inhibition therapy individual patients exist. <sup>89</sup>Zr-bevacizumab PET provides noninvasive tool monitor levels. The aim this study was investigate early antiangiogenic response evaluation...
Non-invasive imaging of angiogenesis could ease the optimization antiangiogenesis treatments for cancer. In this study, we evaluated role VEGF-PET as a biomarker dynamic angiogenic changes in tumors following treatment with kinase inhibitor sunitinib. The effects sunitinib and withdrawal on tumor was investigated using new tracer (89)Zr-ranibizumab well (18)F-FDG PET, (15)O-water PET mouse xenograft models human obtained results were compared growth, VEGF plasma levels immunohistologic...
Abstract Background Endoscopic full-thickness resection (eFTR) is a minimally invasive technique that allows definite diagnosis and treatment for complex colorectal lesions ≤ 30 mm unsuitable conventional endoscopic resection. This study reports clinical outcomes from the Dutch eFTR registry. Methods Consecutive patients undergoing in 20 hospitals were prospectively included. The primary outcome was technical success, defined as macroscopic complete en bloc Secondary were: tumor-free margins...
Small and flat adenomas are known to carry a high miss-rate during standard white-light endoscopy. Increased detection rate may be achieved by molecular fluorescence endoscopy with targeted near-infrared (NIR) fluorescent tracers. The aim of this study was validate vascular endothelial growth factor A (VEGF-A) epidermal receptor (EGFR)–targeted tracers ex vivo colonoscopy an NIR platform. <b>Methods:</b> VEGF-A EGFR expression determined immunohistochemistry on large subset human colorectal...
Adenoma miss rates in colonoscopy are unacceptably high, especially for sessile serrated adenomas / polyps (SSA/Ps) and high-risk populations, such as patients with Lynch syndrome.Detection may be improved by fluorescence molecular endoscopy (FME), which allows morphological visualization of lesions high-definition white-light imaging well fluorescence-guided identification a specific marker.In clinical proof-of-principal study, we investigated FME colorectal adenoma detection, using...
Complete endoscopic resection and accurate histological evaluation for T1 colorectal cancer (CRC) are critical in determining subsequent treatment. Endoscopic full-thickness (eFTR) is a new treatment option CRC < 2 cm. We aimed to report clinical outcomes short-term results.Consecutive eFTR procedures CRC, prospectively recorded our national registry between November 2015 April 2020, were retrospectively analyzed. Primary technical success R0 resection. Secondary risk assessment, curative...