A5025786255- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Neurological disorders and treatments
- Single-cell and spatial transcriptomics
- Genetic Neurodegenerative Diseases
- CAR-T cell therapy research
- Parkinson's Disease Mechanisms and Treatments
- Systemic Sclerosis and Related Diseases
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Aortic aneurysm repair treatments
- Pancreatic and Hepatic Oncology Research
- RNA regulation and disease
- IL-33, ST2, and ILC Pathways
- Neurogenesis and neuroplasticity mechanisms
- interferon and immune responses
- Viral Infections and Immunology Research
- Genetic and Kidney Cyst Diseases
- Chromatin Remodeling and Cancer
- Radiomics and Machine Learning in Medical Imaging
- Cancer Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Dermatologic Treatments and Research
- Evaluation Methods in Various Fields
Xi'an Jiaotong University
2021-2025
Ministry of Education of the People's Republic of China
2025
University of Georgia
2017-2023
Institute of Infection and Immunity
2022
Central Hospital of Wuhan
2015-2017
Huazhong University of Science and Technology
2017
Cathay General Hospital
2009
Taipei Medical University
2009
Chung Shan Medical University Hospital
2006
Taipei Veterans General Hospital
2001-2004
Macrophage activation plays a critical role in abdominal aortic aneurysm (AAA) development. However, molecular mechanisms controlling macrophage and vascular inflammation AAA remain largely unknown. The objective of the study was to identify novel underlying adenosine deaminase acting on RNA (ADAR1) function formation.
Both innate and adaptive immune system undergo evolution from low to high vertebrates. Due the limitation of conventional approaches in identifying broader spectrum cells molecules various vertebrates, it remains unclear how evolve among Here, we utilized carry out comparative transcriptome analysis across seven vertebrate species. Single-cell RNA sequencing (scRNA-seq). We uncovered both conserved species-specific profiling gene expression immunity. Macrophages exhibited highly-diversified...
Abstract A genetic analysis identified 2 patients, approximately one‐tenth of our patients with familial parkinsonism, who had expanded trinucleotide repeats in SCA2 genes. The reduction 18 F‐dopa distribution both the putamen and caudate nuclei confirmed that nigrostriatal dopaminergic system was involved parkinsonian mutation.
Systemic sclerosis (SSc) is a complex, multiorgan autoimmune disease of unknown etiology. Manifestation the results from an interaction three key pathologic features including irregularities antigen-specific immune system and non-specific system, resulting in autoantibody production, vascular endothelial activation small blood vessels, tissue fibrosis as result fibroblast dysfunction. Given heterogeneity clinical presentation disease, lack universal models has impeded adequate testing...
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P0-Cre and Wnt1-Cre mouse lines have been widely used in combination with loxP-flanked mice to label genetically modify neural crest (NC) cells their derivatives. has regarded as the gold standard there concerns about specificity of because it is not clear timing spatial distribution transgene labeling NC at early embryonic stages. We re-visited models embryos a focus on cranial NC. found that R26-lacZ Cre reporter responded activity more reliably than CAAG-lacZ during embryogenesis....
Abstract Functional CD8 + T cell immunity is essential for immune surveillance and host defense against infection tumors. Epigenetic mechanisms, particularly RNA modification, in controlling response not fully elucidated. Here, by cell-specific deletion of fat mass obesity-associated protein (FTO), a critical N6-methyladenosine (m 6 A) demethylase, we revealed that FTO was indispensable adequate protective function. ablation led to considerable death activated cells, which attributed...
Transforming growth factor-β (TGF-β)-induced fibroblast activation is a key pathological event during tissue fibrosis. Long noncoding RNA (lncRNA) class of versatile gene regulators participating in various cellular and molecular processes. However, the function lncRNA still poorly understood. In this study, we identified arrest-specific transcript 5 (GAS5) as novel regulator for TGF-β-induced activation. GAS5 expression was downregulated cultured fibroblasts by TGF-β resident from...
Upon virus infection, CD8 + T cell accumulation is tightly controlled by simultaneous proliferation and apoptosis. However, it remains unclear how TCR signal coordinates these events to achieve expansion effector differentiation. We found that cell–specific deletion of nuclear helicase Dhx9 led impaired survival, differentiation, viral clearance. Mechanistically, acts as the key regulator ensure LCK- CD3ε-mediated ZAP70 phosphorylation ERK activation protect cells from apoptosis before...
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In aged individuals, age-related changes in immune cells, especially T cell deficiency, are associated with an increased incidence of infection, tumor, and autoimmune disease, as well impaired response to vaccination. However, the features gene expression levels cells still unknown. Our previous study successfully tracked generated from one wave developing thymocytes young age by a lineage-specific inducible Cre-controlled reporter ( TCR δ CreER R26 ZsGreen mouse strain). this study, we...
To determine the association of circulating miR-125a/b expression with risk and disease severity Crohn's (CD), inflammatory cytokines.Plasma samples were collected from patients active CD (A-CD), or in remission (R-CD) healthy controls (HCs). The levels cytokines interleukin-17 (IL-17), tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ) measured by enzyme-linked immunosorbent assay. was assessed quantitative polymerase chain reaction (qPCR).Twenty-nine A-CD patients, 37 R-CD HCs included...
Background Aging has long been thought to be a major risk factor for various types of cancers. However, accumulating evidence indicates increased resistance old animals tumor growth. An in-depth understanding how individuals defend against invasion requires further investigations. Methods We revealed age-associated alterations in tumor-infiltrating immune cells between young and mice using single-cell RNA coupled T cell receptor (TCR) sequencing analysis. Multiple bioinformatics methods were...
Abstract Systemic sclerosis (SSc) is a multisystem autoimmune disorder that characterized by inflammation and fibrosis in the skin internal organs. Previous studies indicate inflammatory cells cytokines play essential roles pathogenesis of SSc; however, mechanisms underlie inflammation-driven development SSc are not fully understood. In this study, we show response gene to complement 32 (RGC32) abundantly expressed mouse macrophages early stage bleomycin-induced SSc. Importantly, RGC32...
Abstract We report on clinical 18 F‐labeled 6‐fluorodopa ( F‐dopa) positron emission tomography (PET) and molecular genetic analyses of an ethnic Chinese family in which three siblings presented with early‐onset Parkinson's disease. As described some parkin patients, neither sleep benefit nor diurnal fluctuation was noted. Interestingly, depression, anxiety, obsessive–compulsive disorders were manifest. The F‐dopa PET scans showed bilateral presynaptic dopaminergic dysfunction without marked...
Abstract Among 242 patients with apparently sporadic Parkinson's disease, a 70‐year‐old man CAG repeat number of 37 in the SCA2 gene was identified. He has remained responsive to levodopa 14 years after onset and had no overt signs suggesting cerebellar dysfunction. Although it is not possible confirm if this patient de novo mutation gene, genetic defect seems be contributing his parkinsonian features further supports concept that sporadic, late‐onset, levodopa‐responsive disease may have...
Abstract Under steady‐state conditions, the pool size of peripheral CD8 + T cells is maintained through turnover and survival. Beyond TCR IL‐7R signals, underlying mechanisms are less well understood. In present study, we found a significant reduction cell proportion in spleens but not thymi mice with cell‐specific deletion Mediator Subunit 1 (Med1). A competitive transfer wild‐type (WT) Med1‐deficient reproduced phenotype same recipients confirmed intrinsic role Med1. Furthermore, observed...
Analyzing changes in regional cerebral blood flow (rCBF) with SPECT complex pain syndrome type 1 (CRPS 1), formerly known as reflex sympathetic dystrophy, is an optimal method for evaluating effective relief. We attempted to investigate the correlation of rCBF relief during treatments blockade and multimodal epidural control.We describe a patient severe CRPS whom conventional treatment failed relieve pain. Combined repeated lumbar blocks long-term morphine, bupivacaine, ketamine...
T-cell development in the thymus undergoes process of differentiation, selective proliferation, and survival from CD4-CD8- double negative (DN) stage to CD4+CD8+ positive (DP) prior formation CD4+ helper CD8+ cytolytic T cells ready for circulation. Each developmental is tightly regulated by sequentially operating molecular networks, which only limited numbers transcription regulators have been deciphered. Here, we identified Zfp335 factor as a new player regulatory network controlling...
Effector CD8+ T cells are crucial players in adaptive immunity for effective protection against invading pathogens. The regulatory mechanisms underlying cell effector differentiation incompletely understood. In this study, we defined a critical role of mediator complex subunit 1 (Med1) controlling and survival during acute bacterial infection. Mice with Med1-deficient exhibited significantly impaired expansion evidently reduced killer lectin-like receptor G1+ terminally differentiated Ly6c+...
As a multisystem autoimmune disorder disease, systemic sclerosis (SSc) is characterized by inflammation and fibrosis in the skin other internal organs. However, mechanisms underlying inflammatory response that drives development of SSc remain largely unknown. ADAR1 heterozygous knockout (AD1+/-) mice myeloid-specific were used to determine function SSc. Histopathological analyses western blot confirmed role bleomycin-induced increased lung fibrosis. In this study, we discover adenosine...
A 43-year-old man had complete AV block and syncope. Echocardiography revealed a cardiac tumor attaching to the interatrial septum protruding into left atrial right cavities. F-18 FDG-PET/CT was performed interpreted with contrast-enhanced CT in similar planes side by side. It remarkably increased glucose metabolism atrium, septum, mass from posterior aspect of pericardium. Regions central necrosis were found but there no evidence pleural lymph node involvement. Endometrial biopsy...
Summary Proper development of taste organs including the tongue and papillae requires interactions with underlying mesenchyme through multiple molecular signaling pathways. The effects bone morphogenetic proteins (BMPs) antagonists are profound, however, tissue‐specific roles distinct receptors largely unknown. Here, we report that constitutive activation (ca) ALK2‐BMP in (marked by Wnt1‐Cre ) caused microglossia—a dramatically smaller misshapen a progressively severe reduction size along...
Memory CD8+ T cells play an essential role in providing effective and lifelong protection against pathogens. Comprehensive transcriptional epigenetic networks are involved modulating memory cell development, but the molecular regulations of formation long-term persistence remain largely unknown. In this study, we show that zinc finger protein 335 (Zfp335) is indispensable for establishment maintenance during acute infections. Mice with Zfp335 deletion exhibit a significant reduction...
Early T-cell development from CD4- CD8- double-negative (DN) stage to CD4+ CD8+ double-positive (DP) in the thymus is regulated through multiple steps involving a batch of sequentially expressed factors. Our preliminary data and recent report showed that AT-rich interaction domain 1A (Arid1a) required for transition DN DP stages, but mechanism not fully understood. In this study, we consolidated conditional deletion Arid1a lineage intrinsically caused developmental blocks DN3 DN4 as well...