A5086997032- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Aldose Reductase and Taurine
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Gut microbiota and health
- Lung Cancer Diagnosis and Treatment
- Bioinformatics and Genomic Networks
- Liver Disease Diagnosis and Treatment
- Parasites and Host Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Endoplasmic Reticulum Stress and Disease
- Gene Regulatory Network Analysis
- Cancer Mechanisms and Therapy
Harry S. Truman Memorial Veterans' Hospital
2019-2024
University of Missouri Health System
2020-2024
University of Missouri
2016-2024
University of Connecticut
2024
Washington University in St. Louis
1995-2024
UConn Health
2024
First Affiliated Hospital of Anhui Medical University
2023-2024
Anhui Medical University
2023-2024
Northeast Normal University
2018-2024
Bio-Medical Science (South Korea)
2023
Abstract The interplay between western diet and gut microbiota drives the development of non-alcoholic fatty liver disease its progression to steatohepatitis. However, specific microbial metabolic mediators contributing steatohepatitis remain be identified. Here, a choline-low high-fat high-sugar diet, representing typical named CL-HFS, successfully induces male mouse with some features human disease, such as hepatic inflammation, steatosis, fibrosis. Metataxonomic metabolomic studies...
Phosphatidylinositol (PI) 3-kinases have been implicated in several aspects of intracellular membrane trafficking, although a detailed mechanism is yet to be established. In this study we demonstrated that wortmannin, specific inhibitor PI 3-kinases, inhibited constitutive endocytosis horseradish peroxidase and transferrin BHK-21 TRVb-1 cells. The IC50 was approximately 40 ng/ml (93 nM). addition, wortmannin blocked the stimulation uptake by small GTPase Rab5 but not GTPase-defective,...
Ceramide, a sphingolipid metabolite, affects T-cell signaling, induces apoptosis of cancer cells, and slows tumor growth in mice. However, it has not been used as chemotherapeutic agent because its cell impermeability precipitation aqueous solution. We developed nanoliposome-loaded C6-ceremide (LipC6) to overcome this limitation investigated effects mice with liver tumors.Immune competent C57BL/6 received intraperitoneal injections carbon tetrachloride intra-splenic oncogenic hepatocytes. As...
Background Minimally invasive radiofrequency ablation (RFA) is used as a first-line treatment option for hepatocellular cancer (HCC) with the weaknesses of incomplete ablation, tumor recurrence, and inferior outcomes. To overcome this limitation, we proposed to develop sunitinib-RFA integrated therapy potential activating anti-HCC immune response. Methods Using our unique murine model, developed novel RFA platform modified human cardiac RF generator. Therapeutic efficacy sunitinib–RFA...
Tumor-associated macrophages (TAMs), as a major and essential component of tumor microenvironment (TME), play critical role in orchestrating pancreatic cancer (PaC) tumorigenesis from initiation to angiogenesis, growth, systemic dissemination, well immunosuppression resistance chemotherapy immunotherapy; however, the intrinsic factors responsible for TAMs reprograming function remain be identified. By performing single-cell RNA sequencing, transforming growth factor-beta-induced protein...
ATF5 loss of function has been shown previously to cause apoptotic cell death in glioblastoma and breast cancer cells but not non-transformed astrocytes human epithelial cells. The mechanism for the type-dependent survival is unknown. We report here that anti-apoptotic factor BCL-2 a downstream target mediates prosurvival C6 glioma MCF-7 binds an ATF5-specific regulatory element adjacent negative P2 promoter, stimulating expression. Highlighting critical role ATF5-dependent survival,...
Background: Colorectal cancer (CRC) metastasectomy improves survival, however most patient develop recurrences. Circulating tumor cells (CTCs) are an independent prognostic marker in stage IV CRC. We hypothesized that CTCs can be enriched during applying different isolation techniques. Methods: 25 CRC patients undergoing liver (16 (64%)) or lung (9 (36%)) were prospectively enrolled (clinicaltrial.gov identifier: NCT01722903). Central venous (liver) radial artery (lung) outflow blood (7.5...
In addition to the FDA-approved definition of a circulating tumor cell (CTC), various CTC phenotypes have been discovered. Epithelial-mesenchymal transition (EMT) cancer cells is directly linked PD-L1 upregulation. The goal study was investigate expression and EMT in CTCs non-small lung (NSCLC) patients, perform an outcome analysis. Prospectively, 7.5 mL peripheral blood collected from 30 NSCLC patients that underwent surgery 15 healthy controls. were enriched by size-based microfilter...
Blockade of the interaction between programmed cell death ligand-1 (PD-L1) and its receptor PD-1 has shown great success in cancer immunotherapy. Peptides possess unique characteristics that give them significant advantages as immune checkpoint inhibitors. However, unfavorable physicochemical properties proteolytic stability profiles limit translation bioactive peptides therapeutic agents. Studies have revealed cyclization improves biological activity linear peptides. In this study, we...
Pancreatic cancer is the sixth leading cause of cancer-related mortality globally. As most common form pancreatic cancer, ductal adenocarcinoma (PDAC) represents up to 95% all cases, accounting for more than 300,000 deaths annually. Due lack early diagnoses and high refractory response currently available treatments, PDAC has a very poor prognosis, with 5-year overall survival rate less 10%. Targeted therapy immunotherapy are highly effective have been used treatment many types cancer;...
Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with very poor prognosis. Despite advancements in treatment strategies, PDAC remains recalcitrant to therapies because patients are often diagnosed at an advanced stage. The stage of characterized by metastasis, which typically renders it unresectable surgery or untreatable chemotherapy. tumor microenvironment (TME) comprises proliferative myofibroblast-like cells and hosts the intense deposition extracellular matrix...
In real-world scientific discovery, human beings always make use of the accumulated prior knowledge with imagination pick select one or a few most promising hypotheses from large and noisy data analysis results. this study, we introduce new type graph structure, text-numeric (TNG), which is defined as entities associations have both text-attributed information numeric information. The TNG an ideal structure model for novel discovery via reasoning because it integrates human-understandable...
Although both the heat shock protein 70 (HSP70) and activating transcription factor 5 (ATF5) have been shown to promote cell survival of transformed cells but not non-transformed cells, relationship two molecules is unknown. Here we show that HSP70 ATF5 are concomitantly up-regulated upon transient down-regulated over prolonged cellular stress apoptotic stimulation in rat C6 glioma human U87 cells. interacts strongly with N-terminal activation domain ATF5, which expected be rigid uniquely...
Transforming growth factor-β (TGF-β)-induced fibroblast activation is a key pathological event during tissue fibrosis. Long noncoding RNA (lncRNA) class of versatile gene regulators participating in various cellular and molecular processes. However, the function lncRNA still poorly understood. In this study, we identified arrest-specific transcript 5 (GAS5) as novel regulator for TGF-β-induced activation. GAS5 expression was downregulated cultured fibroblasts by TGF-β resident from...
Abstract Purpose: Systemic treatments given to patients with non–small cell lung cancer (NSCLC) are often ineffective due drug resistance. In the present study, we investigated patient-derived tumor organoids (PDTO) and matched tissues from surgically treated NSCLC identify repurposing targets overcome resistance toward standard-of-care platinum-based doublet chemotherapy. Experimental Design: PDTOs were established 10 prospectively enrolled non-metastatic resected tumors. compared by...