A5082951214- Tissue Engineering and Regenerative Medicine
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Pelvic floor disorders treatments
- Urological Disorders and Treatments
- Shoulder Injury and Treatment
- Hernia repair and management
- IL-33, ST2, and ILC Pathways
- Inflammatory Bowel Disease
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Nasal Surgery and Airway Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Obstructive Sleep Apnea Research
- Urinary Bladder and Prostate Research
- Vasculitis and related conditions
- Anorectal Disease Treatments and Outcomes
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Health Systems, Economic Evaluations, Quality of Life
- Complement system in diseases
- Psoriasis: Treatment and Pathogenesis
- Pharmaceutical Economics and Policy
Case Western Reserve University
2022-2023
Icahn School of Medicine at Mount Sinai
2017-2019
Cleveland Clinic
2011-2014
Vaginal delivery is a risk factor for stress urinary incontinence (SUI). Mesenchymal stem cells (MSCs) home to injured organs and can facilitate repair. The goal of this study was determine if MSCs pelvic after simulated childbirth injury recovery from SUI via paracrine factors. Three experiments were performed. Eighteen female rats received vaginal distension (VD) or sham VD labeled intravenous (IV) investigate the organs. Whole-organ imaging immunofluorescence performed 1 week later....
Defects in the annulus fibrosus (AF) of intervertebral discs allow nucleus pulposus tissue to herniate causing painful disability. Microdiscectomy procedures remove herniated fragments, but unrepaired defects remain allowing reherniation or progressive degeneration. Cell therapies show promise enhance repair, methods are undeveloped and carriers required prevent cell leakage. To address this challenge, study developed evaluated genipin-crosslinked fibrin (FibGen) as an adhesive carrier...
The local route of stem cell administration utilized presently in clinical trials for stress incontinence may not take full advantage the capabilities these cells. goal this study was to evaluate if intravenously injected mesenchymal cells (MSCs) home pelvic organs after simulated childbirth injury a rat model. Female rats underwent either vaginal distension (VD) or sham VD. All received 2 million GFP-labeled MSCs 1 hour injury. Four 10 days later and muscles were imaged visualization...
Annulus fibrosus (AF) defects from intervertebral disk (IVD) herniation and degeneration are commonly associated with back pain. Genipin-crosslinked fibrin hydrogel (FibGen) is an injectable, space-filling AF sealant that was optimized to match shear properties partially restored IVD biomechanics. This study aimed enhance mechanical behaviors of FibGen more closely compressive, tensile, by adjusting genipin crosslink density creating a composite formulation adding...
Nasal reconstruction is limited by the availability of autologous cartilage. The aim to investigate an adhesive biomaterial for tissue engineering nasal cartilage evaluating mechanical properties hydrogels made fibrin crosslinked with genipin as compared native tissue.Hydrogels fibrin, fibrin-genipin, and fibrin-genipin extracellular matrix (ECM) particles were created evaluated testing determine compression, tensile, shear properties. Rabbit septal was harvested tested in these modalities...
Stimulation of the pudendal nerve or anal sphincter could provide therapeutic options for fecal incontinence with little involvement other organs. The goal this project was to assess effects and stimulation on bladder pressures.Ten virgin female Sprague Dawley rats were randomly allocated control (n = 2), perianal 4), 4) groups. A monopolar electrode hooked placed sphincter. Aballoon catheter inserted into anus measure pressure, a via urethra pressure. Bladder pressures measured different...
You have accessJournal of UrologyStem Cell Research1 Apr 2011176 INTRAVENOUS MESENCHYMAL STEM CELLS HOME TO THE URETHRA AND FACILITATE RECOVERY FROM STRESS URINARY INCONTINENCE AFTER CHILDBIRTH INJURY VIA LOCAL SECRETION OF PARACRINE FACTORS Charuspong Dissaranan, Michelle Cruz, Bradley Gill, Levilester Salcedo, Anne Cotleur, Raul Mendieta, Brian Balog, Matt Kiedrowski, Marc Penn, Howard Goldman, and Margot Damaser DissarananCharuspong Dissaranan Cleveland, OH More articles by this author ,...
Tightly regulated communication between the gastrointestinal epithelium and immune cells in underlying lamina propria is critical for homeostasis inflammation. IL-17C, produced by epithelial after exposure to inflammatory stimuli, facilitates cell-to-cell promoting responses Th17 cells. In this study, we demonstrate that Th17-derived cytokines TNF-α, IL-17A, IL-22 synergistically enhance IL-17C expression both human-transformed colonic cell lines primary non-inflammatory bowel disease...
Abstract Immune dysregulation is a major driver in the pathogenesis of ulcerative colitis (UC), but specific mechanisms disease initiated by adaptive immune system remain unknown. While many studies have focused on contribution individual T cell subsets, disrupted communication between epithelium and cells underlying lamina propria not fully understood UC. This study examines impact isolated from healthy participants primary cultures epithelial derived UC donors. Blood human CD4+ CD8+ were...
You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2012120 MESENCHYMAL STEM CELL (MSC) SECRETED FACTORS FACILITATE PUDENDAL NERVE (PN) RECOVERY AFTER SIMULATED CHILDBIRTH INJURY Charuspong Dissaranan, Emily Holthaus, Michelle Cruz, Brian Balog, Matthew Kiedrowski, Marc Penn, Howard Goldman, and Margot Damaser DissarananCharuspong Dissaranan Cleveland, OH , HolthausEmily Holthaus CruzMichelle Cruz BalogBrian Balog KiedrowskiMatthew Kiedrowski...
Abstract Bidirectional communication between the intestinal epithelial barrier and immune cells in underlying lamina propria is critical to human health. This study investigates impact of T cell subtypes on phenotype survival. Blood-derived CD4+ or CD8+ were isolated from healthy participants using negative magnetic separation co-cultured with colonic stem spheroids suspended Matrigel. A subset was activated by anti-CD3/CD28 cross-linking for 24 h prior co-culture. Only (ACD4) caused...
You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research1 Apr 2011786 NEUROREGENERATIVE TREATMENT AFTER SIMULATED CHILDBIRTH INJURY INCREASES EXPRESSION OF BRAIN DERIVED NEUROTROPHIC FACTOR IN THE EXTERNAL URETHRAL SPHINCTER RATS James Steward, Bradley Gill, Michelle Cruz, Charuspong Dissaranan, Haihong Jiang, Dan Li Lin, Brian Balog, and Margot Damaser StewardJames Steward Cleveland, OH More articles by this author , GillBradley Gill CruzMichelle Cruz...