A5054404133- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Cellular Mechanics and Interactions
- Shoulder Injury and Treatment
- Pregnancy-related medical research
- Erythrocyte Function and Pathophysiology
- Cervical and Thoracic Myelopathy
- Tendon Structure and Treatment
- Protein Tyrosine Phosphatases
- Heat shock proteins research
- Neurosurgical Procedures and Complications
- 3D Printing in Biomedical Research
- Parathyroid Disorders and Treatments
- Ubiquitin and proteasome pathways
- Scoliosis diagnosis and treatment
- Protein Kinase Regulation and GTPase Signaling
- TGF-β signaling in diseases
- Technology Assessment and Management
- Signaling Pathways in Disease
- Traumatic Brain Injury and Neurovascular Disturbances
- Microtubule and mitosis dynamics
- Spinal Hematomas and Complications
- Intracerebral and Subarachnoid Hemorrhage Research
- Myofascial pain diagnosis and treatment
- Healthcare and Venom Research
Icahn School of Medicine at Mount Sinai
2014-2024
University of Maryland, Baltimore
2020-2023
Albert Einstein College of Medicine
2019
Montefiore Medical Center
2019
New York Academy of Sciences
2019
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Cornell University
2014
Tumor cell invasion through the stromal extracellular matrix (ECM) is a key feature of cancer metastasis, and understanding cellular mechanisms invasive migration critical to development effective diagnostic therapeutic strategies. Since highly adaptable physiochemical properties ECM, it define these in context-specific manner. Although extensive work has characterized two- three-dimensional (3D) environments, program employed by cells move native cell-derived microtracks within ECM remains...
There is an unmet clinical need for a biomaterial sealant capable of repairing small annulus fibrosus (AF) defects. Causes these defects include painful intervertebral disc herniations, microdiscectomy procedures, morbidity associated with needle puncture injury from discography, and future nucleus replacement procedures. This study describes the enhancements fibrin gel through genipin crosslinking (FibGen) addition cell adhesion molecules (CAMs), fibronectin collagen. The gel's performance...
Defects in the annulus fibrosus (AF) of intervertebral discs allow nucleus pulposus tissue to herniate causing painful disability. Microdiscectomy procedures remove herniated fragments, but unrepaired defects remain allowing reherniation or progressive degeneration. Cell therapies show promise enhance repair, methods are undeveloped and carriers required prevent cell leakage. To address this challenge, study developed evaluated genipin-crosslinked fibrin (FibGen) as an adhesive carrier...
Abstract Multiple histologic measurements are commonly used to assess degenerative changes in intervertebral disc ( IVD ) structure; however, there is no consensus on which stains offer the clearest visualization of specific areas within . The objective this study was compare multiple tinctorial stains, evaluate their ability highlight structural features IVD, and investigate how they influence capacity implement a degeneration scoring system. Lumbar s from seven human autopsy specimens were...
Ectopic calcifications in intervertebral discs (IVDs) are known characteristics of IVD degeneration that not commonly reported but may be implicated structural failure and dysfunctional cell metabolic responses.This study investigated the novel hypothesis ectopic associated with advanced glycation end products (AGEs) via hypertrophy osteogenic differentiation.Histological analyses human IVDs from several stages revealed areas calcification within nucleus pulposus at cartilage endplate.These...
The downregulation of sclerostin in osteocytes mediates bone formation response to mechanical cues and parathyroid hormone (PTH). To date, the regulation has been attributed exclusively transcriptional Sost gene hours after stimulation. Using mouse models rodent cell lines, we describe rapid, minute-scale post-translational degradation protein by lysosome following load PTH. We present a model, integrating both new established mechanically hormonally activated effectors into regulated...
Adult intervertebral discs (IVDs) have poor endogenous healing capacity, because of their challenging microenvironment and complex mechanical demands, which can result in painful IVD herniation. There are no regenerative strategies available to improve restore its function. Neonatal mice excellent models mammalian regeneration, but there studies the capacity neonatal IVDs. In this study, we developed a model improved inform repair after vivo puncture injuries were performed simulate...
Back pain is a leading cause of disability strongly associated with intervertebral disc (IVD) degeneration. Reducing structural disruption and catabolism in IVD degeneration remains an important clinical challenge. Pro-oxidant structure-modifying advanced glycation end-products (AGEs) contribute to obesity diabetes, which are increased back pain, accumulate tissues due hyperglycemia or ingestion foods processed at high heat. Collagen-rich IVDs particularly susceptible AGE accumulation their...
Bone regulates its mass and quality in response to diverse mechanical, hormonal, local signals. The bone anabolic or catabolic responses these signals are often received by osteocytes, which then coordinate the activity of osteoblasts osteoclasts on surfaces. We previously established that calcium/calmodulin-dependent kinase 2 (CaMKII) is required for osteocytes respond some cues vitro. However, a role CaMKII physiology vivo largely undescribed. Here, we show conditional codeletion most...
Abstract Intervertebral disc (IVD) injuries are a cause of degenerative changes in adults which can lead to back pain, leading disability. We developed model neonatal IVD regeneration with full functional restoration and investigate the cellular dynamics underlying this unique healing response. employed genetic lineage tracing mice using Scleraxis ( Scx ) Sonic hedgehog Shh fate-map annulus fibrosus (AF) nucleus pulposus (NP) cells, respectively. Results indicate AF after severe herniation...
Abstract Purpose Neutral zone (NZ) parameters in spinal biomechanics studies are sensitive to instability, disc degeneration, and repair. Multiple methods the literature quantify NZ, yet no consensus exists on applicability comparability of methods. This study compares five different NZ quantification using two load‐deflection profiles. Methods Rat caudal lumbar motion segments were tested axial rotation generate curves with profiles exhibiting prominent distinction between elastic regions...
ABSTRACT Back pain is a leading cause of disability strongly associated with intervertebral disc (IVD) degeneration. Reducing structural disruption and catabolism in IVD degeneration remains an important clinical challenge. Pro-oxidant structure-modifying advanced glycation end-products (AGEs) contribute to obesity diabetes, which are increased back pain, accumulate tissues due hyperglycemia or ingestion foods processed at high heat. Collagen-rich IVDs particularly susceptible AGE...
Objective The underlying mechanisms and molecular factors influencing intervertebral disc (IVD) homeostasis degeneration remain clinically relevant. Tenomodulin (Tnmd) chondromodulin (Chm1) are antiangiogenic transmembrane glycoproteins, with cleavable C-terminus, expressed by IVD cells that implicated in the onset of degenerative processes. We evaluate organ-level biomechanical impact knocking out Tnmd alone, Chm1, simultaneously. Design Caudal (c5-8) lumbar vertebrae (L1-4) skeletally...
Intervertebral disc (IVD) defects heal poorly and can cause back pain disability. We identified that IVD herniation injury heals regeneratively in neonatal mice until postnatal day 14 (p14) shifts to fibrotic healing by p28. This age coincides with the shift expansive growth from cell proliferation matrix elaboration, implicating collagen crosslinking.
Bone is a dynamic tissue that adapts to changes in its mechanical environment. Mechanical stimuli pressurize interstitial fluid the lacunar-canalicular system within bone matrix, causing shear stress (FSS) across embedded, mechano-sensitive osteocytes. Therefore, modeling this stimulus vitro vital for identifying mechano-transduction cascades contribute regulation of mechano-responsive proteins, such as Wnt/β-catenin antagonist, sclerostin, which reduced response FSS. Recently, we reported...