A5011457012- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Tendon Structure and Treatment
- Orthopedic Surgery and Rehabilitation
- Shoulder Injury and Treatment
- Pregnancy-related medical research
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Coronary Interventions and Diagnostics
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Elasticity and Material Modeling
- Bone health and osteoporosis research
- Knee injuries and reconstruction techniques
- Pain Mechanisms and Treatments
- Insect and Arachnid Ecology and Behavior
- Bone fractures and treatments
- Sports injuries and prevention
- Plant Toxicity and Pharmacological Properties
- Anesthesia and Pain Management
- Cell Adhesion Molecules Research
- Bone health and treatments
- Hippo pathway signaling and YAP/TAZ
- Biomarkers in Disease Mechanisms
- Cell death mechanisms and regulation
Icahn School of Medicine at Mount Sinai
2014-2025
New York Proton Center
2024
Mount Sinai Hospital
2022
Mount Sinai Health System
2021
City College of New York
2012-2015
City University of New York
2014
City College
2014
Albert Einstein College of Medicine
2002
Osteocyte apoptosis is spatially and temporally linked to bone fatigue-induced microdamage subsequent intracortical remodeling. Specifically, osteocytes surrounding fatigue microcracks in undergo apoptosis, those regions containing apoptotic co-localize exactly with areas subsequently resorbed by osteoclasts. Here we tested the hypothesis that osteocyte a key controlling step activation and/or targeting of osteoclastic resorption after fatigue. We carried out vivo loading ulna from 4-...
Using 2.1-µm high-resolution microcomputed tomography, we have examined the spatial distribution, clustering, and shape of nearly 35,000 microcalcifications (µCalcs) ≥ 5 µm in fibrous caps 22 nonruptured human atherosclerotic plaques. The vast majority these µCalcs were <15 invisible at previously used 6.7-µm resolution. A greatly simplified 3D finite element analysis has made it possible to quickly analyze which thousands minute inclusions are potentially dangerous. We show that enhancement...
Abstract To date, the cell and molecular mechanisms regulating tendon healing are poorly understood. Here, we establish a novel model of regeneration using neonatal mice show that neonates heal via formation ‘neo-tendon’ differentiates along specific lineage with functional restoration gait mechanical properties. In contrast, adults fibrovascular scar, aberrant differentiation toward cartilage bone, persistently impaired function. Lineage tracing identified intrinsic recruitment Scx-lineage...
The role of microcalcifications (μCalcs) in the biomechanics vulnerable plaque rupture is examined. Our laboratory previously proposed (Ref. 44 ), using a very limited tissue sample, that μCalcs embedded fibrous cap proper could significantly increase instability. This study has been greatly expanded. Ninety-two human coronary arteries containing 62 fibroatheroma were examined high-resolution microcomputed tomography at 6.7-μm resolution and undecalcified histology with special emphasis on...
We characterized the differentiation of rat bone marrow-derived mesenchymal stem cells (BM-MSCs) into tenocyte-like in response to morphogenetic protein-12 (BMP-12). BM-MSCs were prepared from Sprague-Dawley rats and cultured as monolayers. Recombinant BMP-12 treatment (10 ng/ml) for 12 hours vitro markedly increased expression tenocyte lineage markers scleraxis (Scx) tenomodulin (Tnmd) over 14 days. Treatment with a further 12-hour period had no additional effect. Colony formation assays...
Abstract The mechanical and microstructural bases of tendon fatigue, by which damage accumulates contributes to degradation, are poorly understood. To investigate the fatigue process, rat flexor digitorum longus tendons were cyclically loaded (1–16 N) until reaching one three levels damage, defined as peak clamp‐to‐clamp strain magnitudes representing key intervals in life: i) Low (6.0%–7.0%); ii) Moderate (8.5%–9.5%); iii) High (11.0%–12.0%). Stiffness, hysteresis, assessed diagnostically...
Objective Diabetes and low back pain are debilitating diseases modern epidemics. obesity also highly correlated with intervertebral disc (IVD) degeneration pain. Advanced-glycation-end-products (AGEs) increase reactive-oxygen-species (ROS) inflammation, one cause for early development of diabetes mellitus. We hypothesize that results in accumulation AGEs spines associated spinal pathology via increased catabolism. present a mouse model showing that: 1) induces pathological changes to...
Painful musculoskeletal disorders such as intervertebral disc (IVD) degeneration associated with chronic low back pain (termed "Discogenic pain", DBP), are a significant socio-economic burden worldwide and contribute to the growing opioid crisis. Yet there very few if any successful interventions that can restore tissue's structure function while also addressing symptomatic pain. Here we have developed novel non-viral gene therapy, using engineered extracellular vesicles (eEVs) deliver...
Both overuse and disuse of joints up-regulate matrix metalloproteinases (MMPs) in articular cartilage cause tissue degradation; however, moderate (physiological) loading maintains integrity. Here, we test whether CBP/p300-interacting transactivator with ED-rich tail 2 (CITED2), a mechanosensitive transcriptional coregulator, mediates this chondroprotective effect mechanical loading. In vivo, hind-limb immobilization Sprague-Dawley rats up-regulates MMP-1 causes rapid, histologically...
Mesenchymal stem cell (MSC)-based treatments have shown promise for improving tendon healing and repair. MSCs the potential to differentiate into multiple lineages in response select chemical physical stimuli, including tenocytes. Cell elongation cytoskeletal tension been be instrumental process of MSC differentiation. Previous studies that inhibition stress fiber formation leads default toward an adipogenic lineage, which suggests fibers are required sense environmental factors can induce...
Intramuscular fatty infiltration in muscle injuries and diseases, caused by aberrant adipogenesis of fibro-adipogenic progenitors, negatively impacts function. delivery wingless-type MMTV integration site family 7a (WNT7A) offers a promising strategy to stimulate regeneration, but its effects on adipogenic conversion progenitors remain unknown. Here, we show that WNT7A decreases (FAPs) inducing nuclear localization Yes-associated protein (YAP) through Rho β-CATENIN-independent manner...
Tendon-to-bone repair remains a surgical challenge. Although bone tunnel fixation is common technique whereby soft tissue expected to heal against interface, contemporary methods have yet recapitulate biomechanical similarity the native enthesis. In this study, we aimed understand how inside-out longitudinal tendon inversion affects healing with hypothesis that removes gliding epitenon surface facilitate interface healing. Forty male Sprague-Dawley rats underwent either tenodesis (control...
Background Peripheral nerve injury commonly results in long-term disability and pain for patients. Recovery after traction or crush is unpredictable depends on the degree of injury. Our inability to accurately assess severity hampers our ability predict chances recovery need surgical intervention form neurolysis, repair, graft. An investigation into histologic sequence mechanics failure under tension may help process assessing injury, prognosis recovery, treatment. Questions/purposes Using...