A5042590199- Virus-based gene therapy research
- Electron Spin Resonance Studies
- Cancer Research and Treatments
- Herpesvirus Infections and Treatments
- Lanthanide and Transition Metal Complexes
- Trace Elements in Health
- Advanced MRI Techniques and Applications
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- MicroRNA in disease regulation
- Atrial Fibrillation Management and Outcomes
- Animal Virus Infections Studies
- Glioma Diagnosis and Treatment
- Sulfur Compounds in Biology
- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Analytical Chemistry and Sensors
- Heat shock proteins research
- Bioactive Compounds and Antitumor Agents
- Redox biology and oxidative stress
- Cardiac electrophysiology and arrhythmias
- Advanced biosensing and bioanalysis techniques
- Aortic aneurysm repair treatments
The Ohio State University Wexner Medical Center
2011-2024
The Ohio State University
2011-2024
Imaging Center
2016-2024
Weatherford College
2023
Sutter Davis Hospital
2023
Standard Imaging (United States)
2017-2022
Lung Institute
2008-2020
Metabolism and Renal Physiology
2013
Northwestern University
2012
Neurological Surgery
2012
AimsThe complex architecture of the human atria may create physical substrates for sustained re-entry to drive atrial fibrillation (AF). The existence sustained, anatomically defined AF drivers in humans has been challenged partly due lack simultaneous endocardial–epicardial (Endo–Epi) mapping coupled with high-resolution 3D structural imaging.
MicroRNAs (miRNAs), single-stranded non-coding RNAs, influence myriad biological processes that can contribute to cancer. Although tumor-suppressive and oncogenic functions have been characterized for some miRNAs, the majority of microRNAs not investigated their ability promote modulate tumorigenesis. Here, we established miR-191/425 cluster is transcriptionally dependent on host gene, DALRD3, hormone 17β-estradiol (estrogen or E2) controls expression both DALRD3. MiR-191/425 locus...
Autonomic dysreflexia (AD), a potentially dangerous complication of high-level spinal cord injury (SCI) characterized by exaggerated activation autonomic (sympathetic) reflexes, can cause pulmonary embolism, stroke, and, in severe cases, death. People with SCI also are immune compromised, rendering them more susceptible to infectious morbidity and mortality. The mechanisms underlying postinjury suppression not known. Data presented herein indicate that AD causes suppression. Using <i>in...
Structural remodeling of human atria plays a key role in sustaining atrial fibrillation (AF), but insufficient quantitative analysis structure impedes the treatment AF. We aimed to develop novel 3-dimensional (3D) structural and computational simulation tool that could reveal contributors reentrant AF drivers.High-resolution panoramic epicardial optical mapping coronary-perfused explanted intact (63-year-old woman, chronic hypertension, heart weight 608 g) was conducted during sinus rhythm...
In older adults, the loss of muscle strength (dynapenia) and mass (sarcopenia) are important contributors to physical function. We sought investigate dynapenia, sarcopenia, motor unit function in aging mice. C57BL/6J mice were analyzed with cross-sectional (males: 3 vs. 27 months; males females: 8 12 20 months) longitudinal studies 10–25 using vivo electrophysiological measures connectivity (triceps surae compound action potential number estimation), plantar flexion torque, magnetic...
We report that EF24, a synthetic compound 3,5-bis(2-flurobenzylidene)piperidin-4-one, greatly inhibits cisplatin-resistant (CR) human ovarian cancer cell proliferation. The inhibitory effect of EF24 on proliferation is associated with G(2)/M phase cycle arrest and increased checkpoint protein (pp53, p53, p21) levels. Within 24 h following treatment, induced apoptosis in CR cells. was partially blocked by the general caspase inhibitor z-VAD. 12 h, membranous FasL expression, consistent...
Signal transducer and activator of transcription 3 (STAT3) is activated in a variety human cancers, including ovarian cancer. The molecular mechanism by which the STAT3 cancer cells poorly understood. We observed that xenograft tumors (A2780) mice were severely hypoxic (pO(2) approximately 2 mmHg). further exposure significantly increased phosphorylation (pSTAT3) at Tyr705 residue A2780 cell line. pSTAT3 (Tyr705) level was highly dependent on cellular oxygenation levels, with significant...
Painful musculoskeletal disorders such as intervertebral disc (IVD) degeneration associated with chronic low back pain (termed "Discogenic pain", DBP), are a significant socio-economic burden worldwide and contribute to the growing opioid crisis. Yet there very few if any successful interventions that can restore tissue's structure function while also addressing symptomatic pain. Here we have developed novel non-viral gene therapy, using engineered extracellular vesicles (eEVs) deliver...
Ovarian cancer is a gynecological malignancy that commonly treated by cytoreductive surgery followed cisplatin treatment. However, the treatment, although successful initially, not effective in treatment of recurrent disease invariably surfaces within few months initial The refractory behavior attributed to increased levels cellular thiols apparently caused This observation prompted us choose cytotoxic drug whose activity potentiated with enhanced specificity toward thiol-rich...
Abstract The purpose of this study was to evaluate the anticancer potency and mechanism a novel difluorodiarylidenyl piperidone (H-4073) its N-hydroxypyrroline modification (HO-3867) in human ovarian cancer. Studies were done using established cancer cell lines (A2870, A2780cDDP, OV-4, SKOV3, PA-1, OVCAR3) as well murine xenograft tumor (A2780) model. Both compounds comparably significantly cytotoxic A2780 cells. However, HO-3867 showed preferential toxicity toward cells while sparing...
Here, we describe the construction and testing of a novel herpes simplex virus type 1 (HSV-1) derived oncolytic (OV): 34.5ENVE (viral ICP34.5 Expressed by Nestin promotor Vstat120 Expressing), for treatment cancer. This showed significant glioma-specific killing antiangiogenic effects in vitro vivo. Treatment subcutaneous intracranial glioma-bearing mice with increase median survival four different glioma models. Histology dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI)...
Alzheimer's disease (AD) is a progressive neurodegenerative characterized clinically by cognitive decline and memory loss. The pathological features are amyloid-β peptide (Aβ) plaques intracellular neurofibrillary tangles. Many studies have suggested that oxidative damage induced reactive oxygen species (ROS) an important mechanism for AD progression. Our recent study demonstrated stress could further impair mitochondrial function. In the present study, we adopted transgenic mouse model of...
Gout is the most prevalent inflammatory arthritis. To study effects of regular physical activity and exercise intensity on inflammation clinical outcome, we examined pathogenesis in an acute model murine gout analyzed human patient data as a function activity.NF-κB-luciferase reporter mice were organized into four groups exercised at 0 m/min (non-exercise), 8 (low-intensity), 11 (moderate-intensity), 15 (high-intensity) for two weeks. Mice subsequently received intra-articular monosodium...
Abstract A novel procedure for in vivo imaging of the oxygen partial pressure (pO 2 ) implanted tumors is reported. The uses electron paramagnetic resonance (EPRI) oxygen‐sensing nanoprobes embedded tumor cells. Unlike existing methods pO quantification, wherein probes are physically inserted at time measurement, new approach cells that preinternalized (labeled) with probes, which become permanently developed tumor. Radiation‐induced fibrosarcoma (RIF‐1) cells, internalized lithium octa‐ n...
Ovarian carcinoma is the leading cause of mortality among gynecological cancers in world. The high rate associated with lack early diagnosis and development drug resistance. antitumor efficacy mechanism NCX-4040, a nitric oxide-releasing aspirin derivative, against ovarian cancer studied.NCX-4040, alone or combination cisplatin (cis-diamminedichloroplatinum, cDDP), was studied cisplatin-sensitive (A2780 WT) cisplatin-resistant cDDP) cell lines as well xenograft tumors grown nude mice....
Fatty acid synthase (FAS) and focal adhesion kinase (FAK), which are overexpressed in a variety of human epithelial tumors, play key role the migration invasion cancer cells. Hence, strategies targeted at inhibiting FAS/FAK proteins may have therapeutic potential for treatment. The goal present study was to determine effect HO-3867, synthetic compound, on migratory ability ovarian cells understand mechanistic pathways including involvement FAS, FAK, associated signaling proteins. done using...
Stem cell therapy for myocardial tissue repair is limited by the poor survival of transplanted cells, possibly because inadequate supply oxygen and nutrients. The purpose this study was to assess oxygenation level functional recovery after allogenic transplantation mesenchymal stem cells (MSC) in a rat model infarction (MI). Myocardial tension (Po(2)) measured electron paramagnetic resonance oximetry using an implantable oxygen-sensing spin probe (OxySpin). MSCs incubated with OxySpins...
Phosphatase and tensin homolog (PTEN), a tumor suppressor gene, has been shown to play vital role in vascular smooth muscle cell (SMC) proliferation hence is potential therapeutic target inhibit remodeling. The goal of this study was evaluate the efficacy mechanism HO-3867 [((3<i>E</i>,5<i>E</i>)-3,5-bis[(4-fluorophenyl)methylidene]-1-[(1-hydroxy-2,2,5,5-tetramethyl-2,5-dihydro-1<i>H</i>-pyrrol-3-yl)methyl]piperidin-4-one)], new synthetic curcuminoid, inhibition SMC restenosis. Experiments...
Abstract Metallothioneins (MT) are potent scavengers of free radicals that silenced in primary hepatocellular carcinomas (HCC) human and rodent origin. To examine whether loss MT promotes hepatocarcinogenesis, male Mt-1 Mt-2 double knockout (MTKO) wild-type (WT) mice were exposed to diethylnitrosamine (DEN) induction HCC was monitored at 23 33 weeks. The size number liver tumors, the ratio between body weight, damage markedly elevated MTKO both time points compared with WT mice. At weeks,...
Hypoxia, which is commonly observed in many solid tumors, a major impediment to chemo- or radiation therapy. Hypoxia also known overexpress/activate signal transducer and activator of transcription 3 (STAT3) leading tumor progression as well drug resistance. We hypothesized that increased oxygenation the hypoxic may have an inhibitory effect on STAT3 activation hence tumor-growth inhibition. Mice containing human ovarian cancer xenograft were exposed hyperbaric oxygen (HBO; 100% oxygen; 2...
Medulloblastoma is the most common type of pediatric brain tumor. Although numerous factors influence patient survival rates, more than 30% all cases will ultimately be refractory to conventional therapies. Current standards care are also associated with significant morbidities, giving impetus for development new treatments. We have previously shown that oncolytic measles virotherapy effective against medulloblastoma, leading prolongation and even cures in mouse xenograft models localized...