A5000810476- Blood Coagulation and Thrombosis Mechanisms
- Complement system in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- CAR-T cell therapy research
- Blood properties and coagulation
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Dermatological and COVID-19 studies
- Vasculitis and related conditions
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Atrial Fibrillation Management and Outcomes
- Immune Response and Inflammation
- Blood groups and transfusion
- S100 Proteins and Annexins
- T-cell and B-cell Immunology
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
Sanquin
2022-2024
University Medical Center Utrecht
2021-2022
Utrecht University
2021-2022
University of Amsterdam
2022
Neutrophils are the most abundant innate immune cells in circulation and they first recruited to sites of infection or inflammation. Almost half intracellular protein content neutrophils consists S100A8 S100A9, though there has been controversy about their actual localization. Once released extracellularly, these proteins thought act as damage-associated molecular patterns (DAMPs), mechanism action is not well understood. These S100 mainly form heterodimers (S100A8/A9, also known...
Severe acute respiratory syndrome coronavirus 2 infection is associated with an increased incidence of thrombosis.By studying the fibrin network structure disease 2019 (COVID-19) patients, we aimed to unravel pathophysiological mechanisms that contribute this risk thrombosis. This may optimal prevention and treatment COVID-19 related thrombosis.In case-control study, collected plasma samples from intensive care unit (ICU) patients COVID-19, without confirmed thrombosis, between April...
Tumor-associated antigens and their derived peptides constitute an opportunity to design off-the-shelf mainline or adjuvant anti-cancer immunotherapies for a broad array of patients. A performant rational antigen selection pipeline would lay the foundation immunotherapy trials with potential enhance treatment, tremendously benefiting patients suffering from rare, understudied cancers. We present experimentally validated, data-driven computational that selects ranks in multipronged approach....
Significance Serine protease inhibitors (SERPINs) regulate vital physiological processes. Considerable research effort has gone into the development of SERPINs as therapeutic agents. One goals is SERPIN modification for improved specificity toward desired target(s). The and potency can be altered by changing their reactive center loop (RCL). Currently, predicting these properties a RCL sequence almost impossible. By characterizing 160 α1-antitrypsin variants with single–amino acid...
Thrombomodulin on endothelial cells can form a complex with thrombin. This has both anticoagulant properties, by activating protein C, and clot-protective thrombin-activatable fibrinolysis inhibitor (TAFI). Activated TAFI (TAFIa) inhibits plasmin-mediated fibrinolysis.TAFIa inhibition is considered potential antithrombotic strategy. So far, this goal been pursued developing compounds that directly inhibit TAFIa. In contrast, we here describe variable domain of heavy-chain-only antibody (VhH)...
Understanding antigen-specific T-cell responses is crucial for advancing immunotherapies and vaccine development. This study proposes a novel approach combining two complementary assays: the 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay (tracking proliferation over 0-48 h) VPD450 dye dilution 4-6 days). Integrating these techniques provides additional insights into kinetics. Both assays were independently optimized using anti-CD3 anti-CD28 polyclonal T cell stimulation. 1 μM suitable...