Betaglycan, also known as the transforming growth factor-β (TGF-β) type III receptor, is a membrane-anchored proteoglycan that binds TGF-β via its core protein. Deletion mutagenesis analysis has revealed two regions of betaglycan ectodomain capable binding TGF-β: one at amino-terminal half, endoglin-related region (López-Casillas, F., Payne, H., Andres, J. L., and Massagué, (1994) <i>J. Cell Biol.</i> 124, 557–568), other carboxyl-terminal uromodulin-related (Pepin, M.-C., Beauchemin, M.,...

Abstract: The endogenous opioid receptor‐like 1 (ORL ) ligand, nociceptin/orphanin FQ (FGGFTGARKSARKLANQ), a heptadecapeptide structurally resembling dynorphin A, has recently been identified. wide distribution of ORL mRNA and precursor in the CNS, particularly limbic system regions several areas known to be involved pain perception, suggests that is potentially endowed with various central functions. In general, activation and/or inactivation regulatory peptides occur through action cell...

An important step of innate immune response is the recruitment polymorphonuclear leukocytes (PMN) to injured tissues through chemotactic molecules. Galectins, a family endogenous lectins, participate in numerous functions such as lymphoid cell migration, homing, cell-cell and cell-matrix interactions. Particularly, galectin-3 (Gal-3) -9 have been implicated modulation acute chronic inflammation by inducing directional migration monocytes/macrophages eosinophils, whereas Gal-1 considered...

Betaglycan is an accessory receptor of members the transforming growth factor-β (TGF-β) superfamily, which regulates their actions through ligand-dependent interactions with type II receptors. A natural soluble form betaglycan found in serum and extracellular matrices. Soluble betaglycan, prepared as a recombinant protein using baculoviral expression system, inhibits TGF-β. Because its potential use anti-TGF-β therapeutic agent, we have purified characterized betaglycan. Baculoviral...

Betaglycan is a coreceptor for members of the transforming growth factor β (TGF-β) superfamily. Mutagenesis has identified two ligand binding regions, one at membrane-distal and other membrane-proximal half betaglycan ectodomain. Here we show that partial plasmin digestion soluble produces proteolysis-resistant fragments 45 55 kDa, consistent with predicted secondary structure, which indicates an intervening nonstructured linker region separating highly structured N- C-terminal domains....

Betaglycan is an accessory receptor of members the transforming growth factor-β (TGF-β) superfamily, which regulates their actions through ligand-dependent interactions with type II receptors. A natural soluble form betaglycan found in serum and extracellular matrices. Soluble betaglycan, prepared as a recombinant protein using baculoviral expression system, inhibits TGF-β. Because its potential use anti-TGF-β therapeutic agent, we have purified characterized betaglycan. Baculoviral...

In this paper, we show that the 36–45 surface-exposed sequence WYKAELNGKD of growth factor receptor-bound protein 2 (Grb2) N-SH3 domain inhibits interaction between Grb2 and a 97-kDa identified as dynamin. Moreover, peptide GPPPQVPSRPNR from dynamin also blocks binding to proline-rich recognition platform Grb2. Mutations in motif Glu-40 is critical for recognition. These observations were confirmed by immunoprecipitation experiments, carried out using ER 22 cells. It was observed unable...

To determine whether GH and prolactin could be phosphorylated, turkey GH, chicken were incubated in vitro with the catalytic subunit of protein kinase A [gamma-32P]ATP. Phosphorylation was assessed after sodium dodecyl sulphate-polyacrylamide gel electrophoresis, Western blotting autoradiography. Polyacrylamide electrophoresis showed that both purified native phosphorylated under conditions employed. However, glycosylated variant did not appear to labelled. Chicken prolactin, all intensely...

Background: Several studies have confirmed that galectin-1 (Gal-1) plays a role in controlling the immune response because of its pro-apoptotic effect. Although based on rheumatoid arthritis (RA) mouse model suggested crucial for Gal-1 inflammation, clinical data are lacking. We detected presence autoantibodies against galectins blood, but their physiological meaning remains unknown.Objectives: To compare plasma and synovial levels RA patients healthy controls, correlate them with...

Aim of Study:Gonadal hormones exert a profound influence on the development, structure and function sexual organs.The testosterone is one androgens that plays an essential role in development organs male mammals.Therefore, present study was undertaken to evaluate levels developmental pattern penile spines seminiferous tubules during early postnatal life Wistar rats.Methods Materials: At 7,14,21,28,35,42,49, 56, 63 70 days after birth, testicular tissues rats were dissected out fixed for...

Objectives: Systemic lupus erythematosus (SLE) is characterized by the production of multiple autoantibodies and also T-cell dysfunction. CD43 expressed most immune cells, involved in lymphocyte adhesion activation, interacts with galectin-1 (Gal-1). The aim this work was to evaluate plasma levels against Gal-1 as well soluble SLE Mexican mestizo patients, establishing a correlation between these parameters clinical profile.Methods: Serum immunoglobulin (Ig)G were measured enzyme-linked...

The CD43 coreceptor molecule has been shown to participate in lymphocyte adhesion and activation. Leukocyte homotypic aggregation results from a cascade of intracellular signals delivered the cells upon engagement different cell-surface molecules with their natural ligands. This phenomenon requires an active metabolism, reorganization cytoskeleton, relocalization molecules. aim this study was identify some key members signaling leading T following engagement. CD43-mediated lymphocytes...

Abstract The aim of this paper is to evaluate the relationship salivary ammonium levels and presence bacteria with rheumatoid arthritis (RA) clinical disease activity in a cross‐sectional study Mexican patients. From periodontal standpoint, 132 consecutive RA patients fulfilling criteria were evaluated. Ammonia (including peptidyl arginine deiminase activity) evaluated by colorimetric assay Porphyromonas gingivalis , Tannerella forsythia Prevotella intermedia was polymerase chain reaction...

Stage-specific embryonic antigen-4 (SSEA-4) is a marker for the identification of multipotent cells. It also positive in neuroepithelial cells, precursor neural cells (NPC), and human dental pulp The aim this study was to evaluate potential morphodifferentiation histodifferentiation NPC SSEA-4 stem from exfoliated deciduous teeth (SHED).A SHED population culture, SSEA-4, obtained by magnetic cell separation. were characterized immunohistochemistry flow cytometry. Subsequently, neurosphere...

The aim of this study was to determine the levels leptin (Lep) and adiponectin (AdipoQ) in patients with gout its relationship joint inflammatory data and/or metabolic syndrome (MetS) variables, during 1 year follow-up. Forty-one (40 males) diagnosis, attending for first time a rheumatology department, were included. Evaluations performed baseline, at 6 12 months. Variables included following: demographic, clinical laboratory related associated diseases. Lep AdipoQ determinations by ELISA...